Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2248 and EX28DRAFT_2249 are separated by 161 nucleotides EX28DRAFT_2249 and EX28DRAFT_2250 are separated by 9 nucleotides EX28DRAFT_2250 and EX28DRAFT_2251 are separated by 35 nucleotides
EX28DRAFT_2248: EX28DRAFT_2248 - tRNA, at 2,269,951 to 2,270,026
_2248
EX28DRAFT_2249: EX28DRAFT_2249 - tol-pal system protein YbgF, at 2,270,188 to 2,270,982
_2249
EX28DRAFT_2250: EX28DRAFT_2250 - peptidoglycan-associated lipoprotein, at 2,270,992 to 2,271,513
_2250
EX28DRAFT_2251: EX28DRAFT_2251 - tol-pal system beta propeller repeat protein TolB, at 2,271,549 to 2,272,841
_2251
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2270.128 kb on + strand at 2270.128 kb on + strand at 2270.137 kb on - strand at 2270.255 kb on - strand at 2270.315 kb on - strand, within EX28DRAFT_2249 at 2270.315 kb on - strand, within EX28DRAFT_2249 at 2270.401 kb on - strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.432 kb on + strand, within EX28DRAFT_2249 at 2270.450 kb on + strand, within EX28DRAFT_2249 at 2270.466 kb on - strand, within EX28DRAFT_2249 at 2270.546 kb on + strand, within EX28DRAFT_2249 at 2270.573 kb on - strand, within EX28DRAFT_2249 at 2270.686 kb on - strand, within EX28DRAFT_2249 at 2270.689 kb on + strand, within EX28DRAFT_2249 at 2270.689 kb on + strand, within EX28DRAFT_2249 at 2270.690 kb on - strand, within EX28DRAFT_2249 at 2270.690 kb on - strand, within EX28DRAFT_2249 at 2270.816 kb on - strand, within EX28DRAFT_2249 at 2270.889 kb on + strand, within EX28DRAFT_2249 at 2270.889 kb on + strand, within EX28DRAFT_2249 at 2270.973 kb on + strand at 2270.974 kb on - strand at 2270.993 kb on + strand at 2270.993 kb on + strand at 2270.996 kb on + strand at 2271.049 kb on + strand, within EX28DRAFT_2250 at 2271.120 kb on - strand, within EX28DRAFT_2250 at 2271.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,270,128 + -0.3 2,270,128 + +0.3 2,270,137 - +0.0 2,270,255 - +0.1 2,270,315 - EX28DRAFT_2249 0.16 +0.3 2,270,315 - EX28DRAFT_2249 0.16 -0.1 2,270,401 - EX28DRAFT_2249 0.27 -0.0 2,270,411 + EX28DRAFT_2249 0.28 +1.8 2,270,411 + EX28DRAFT_2249 0.28 +0.8 2,270,411 + EX28DRAFT_2249 0.28 +0.2 2,270,412 - EX28DRAFT_2249 0.28 -0.1 2,270,412 - EX28DRAFT_2249 0.28 +0.1 2,270,412 - EX28DRAFT_2249 0.28 -0.0 2,270,432 + EX28DRAFT_2249 0.31 -0.4 2,270,450 + EX28DRAFT_2249 0.33 +0.3 2,270,466 - EX28DRAFT_2249 0.35 +0.2 2,270,546 + EX28DRAFT_2249 0.45 -0.1 2,270,573 - EX28DRAFT_2249 0.48 -0.5 2,270,686 - EX28DRAFT_2249 0.63 -0.6 2,270,689 + EX28DRAFT_2249 0.63 +0.1 2,270,689 + EX28DRAFT_2249 0.63 +0.7 2,270,690 - EX28DRAFT_2249 0.63 +0.2 2,270,690 - EX28DRAFT_2249 0.63 +0.0 2,270,816 - EX28DRAFT_2249 0.79 +0.8 2,270,889 + EX28DRAFT_2249 0.88 +0.7 2,270,889 + EX28DRAFT_2249 0.88 +0.5 2,270,973 + -0.2 2,270,974 - -0.3 2,270,993 + -0.3 2,270,993 + +0.4 2,270,996 + -1.1 2,271,049 + EX28DRAFT_2250 0.11 -1.5 2,271,120 - EX28DRAFT_2250 0.25 -2.2 2,271,544 - +0.2
Or see this region's nucleotide sequence