Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2220 and EX28DRAFT_2221 are separated by 127 nucleotides EX28DRAFT_2221 and EX28DRAFT_2222 are separated by 19 nucleotides EX28DRAFT_2222 and EX28DRAFT_2223 overlap by 4 nucleotides
EX28DRAFT_2220: EX28DRAFT_2220 - urocanate hydratase (EC 4.2.1.49), at 2,243,724 to 2,245,409
_2220
EX28DRAFT_2221: EX28DRAFT_2221 - histidine utilization repressor, proteobacterial, at 2,245,537 to 2,246,271
_2221
EX28DRAFT_2222: EX28DRAFT_2222 - formiminoglutamase (EC 3.5.3.8), at 2,246,291 to 2,247,229
_2222
EX28DRAFT_2223: EX28DRAFT_2223 - imidazolonepropionase (EC 3.5.2.7), at 2,247,226 to 2,248,449
_2223
Position (kb)
2246
2247
2248 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2245.397 kb on + strand at 2245.449 kb on + strand at 2245.449 kb on + strand at 2245.450 kb on - strand at 2245.450 kb on - strand at 2245.450 kb on - strand at 2245.508 kb on + strand at 2245.532 kb on - strand at 2245.538 kb on + strand at 2245.660 kb on - strand, within EX28DRAFT_2221 at 2245.748 kb on + strand, within EX28DRAFT_2221 at 2245.749 kb on - strand, within EX28DRAFT_2221 at 2245.749 kb on - strand, within EX28DRAFT_2221 at 2245.772 kb on + strand, within EX28DRAFT_2221 at 2245.772 kb on + strand, within EX28DRAFT_2221 at 2246.396 kb on + strand, within EX28DRAFT_2222 at 2246.408 kb on - strand, within EX28DRAFT_2222 at 2246.523 kb on + strand, within EX28DRAFT_2222 at 2246.527 kb on + strand, within EX28DRAFT_2222 at 2246.527 kb on + strand, within EX28DRAFT_2222 at 2246.704 kb on - strand, within EX28DRAFT_2222 at 2246.949 kb on + strand, within EX28DRAFT_2222 at 2246.998 kb on + strand, within EX28DRAFT_2222 at 2246.998 kb on + strand, within EX28DRAFT_2222 at 2247.090 kb on - strand, within EX28DRAFT_2222 at 2247.090 kb on - strand, within EX28DRAFT_2222 at 2247.230 kb on - strand at 2247.230 kb on - strand at 2247.249 kb on + strand at 2247.258 kb on - strand at 2247.281 kb on + strand at 2247.641 kb on + strand, within EX28DRAFT_2223 at 2247.648 kb on + strand, within EX28DRAFT_2223 at 2247.649 kb on - strand, within EX28DRAFT_2223 at 2247.672 kb on - strand, within EX28DRAFT_2223 at 2247.822 kb on - strand, within EX28DRAFT_2223 at 2247.823 kb on + strand, within EX28DRAFT_2223 at 2247.823 kb on + strand, within EX28DRAFT_2223 at 2247.851 kb on - strand, within EX28DRAFT_2223 at 2247.876 kb on - strand, within EX28DRAFT_2223 at 2248.054 kb on - strand, within EX28DRAFT_2223
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,245,397 + +0.1 2,245,449 + +0.1 2,245,449 + +2.7 2,245,450 - +0.1 2,245,450 - -0.2 2,245,450 - +0.4 2,245,508 + -0.3 2,245,532 - -0.6 2,245,538 + +0.1 2,245,660 - EX28DRAFT_2221 0.17 -0.5 2,245,748 + EX28DRAFT_2221 0.29 -0.7 2,245,749 - EX28DRAFT_2221 0.29 +0.3 2,245,749 - EX28DRAFT_2221 0.29 +0.8 2,245,772 + EX28DRAFT_2221 0.32 -0.1 2,245,772 + EX28DRAFT_2221 0.32 -0.1 2,246,396 + EX28DRAFT_2222 0.11 -1.0 2,246,408 - EX28DRAFT_2222 0.12 -0.7 2,246,523 + EX28DRAFT_2222 0.25 -0.7 2,246,527 + EX28DRAFT_2222 0.25 -0.8 2,246,527 + EX28DRAFT_2222 0.25 -1.9 2,246,704 - EX28DRAFT_2222 0.44 -0.2 2,246,949 + EX28DRAFT_2222 0.70 -0.7 2,246,998 + EX28DRAFT_2222 0.75 -0.5 2,246,998 + EX28DRAFT_2222 0.75 -1.0 2,247,090 - EX28DRAFT_2222 0.85 +0.1 2,247,090 - EX28DRAFT_2222 0.85 -0.4 2,247,230 - -0.3 2,247,230 - -0.4 2,247,249 + -0.7 2,247,258 - +0.7 2,247,281 + -0.8 2,247,641 + EX28DRAFT_2223 0.34 -1.3 2,247,648 + EX28DRAFT_2223 0.34 +0.4 2,247,649 - EX28DRAFT_2223 0.35 -0.0 2,247,672 - EX28DRAFT_2223 0.36 -1.2 2,247,822 - EX28DRAFT_2223 0.49 -0.3 2,247,823 + EX28DRAFT_2223 0.49 -0.7 2,247,823 + EX28DRAFT_2223 0.49 +0.4 2,247,851 - EX28DRAFT_2223 0.51 -0.2 2,247,876 - EX28DRAFT_2223 0.53 +1.2 2,248,054 - EX28DRAFT_2223 0.68 +0.3
Or see this region's nucleotide sequence