Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2222

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2220 and EX28DRAFT_2221 are separated by 127 nucleotidesEX28DRAFT_2221 and EX28DRAFT_2222 are separated by 19 nucleotidesEX28DRAFT_2222 and EX28DRAFT_2223 overlap by 4 nucleotides EX28DRAFT_2220: EX28DRAFT_2220 - urocanate hydratase (EC 4.2.1.49), at 2,243,724 to 2,245,409 _2220 EX28DRAFT_2221: EX28DRAFT_2221 - histidine utilization repressor, proteobacterial, at 2,245,537 to 2,246,271 _2221 EX28DRAFT_2222: EX28DRAFT_2222 - formiminoglutamase (EC 3.5.3.8), at 2,246,291 to 2,247,229 _2222 EX28DRAFT_2223: EX28DRAFT_2223 - imidazolonepropionase (EC 3.5.2.7), at 2,247,226 to 2,248,449 _2223 Position (kb) 2246 2247 2248Strain fitness (log2 ratio) -2 -1 0 1 2at 2245.397 kb on + strandat 2245.449 kb on + strandat 2245.449 kb on + strandat 2245.450 kb on - strandat 2245.450 kb on - strandat 2245.450 kb on - strandat 2245.508 kb on + strandat 2245.532 kb on - strandat 2245.538 kb on + strandat 2245.660 kb on - strand, within EX28DRAFT_2221at 2245.748 kb on + strand, within EX28DRAFT_2221at 2245.749 kb on - strand, within EX28DRAFT_2221at 2245.749 kb on - strand, within EX28DRAFT_2221at 2245.772 kb on + strand, within EX28DRAFT_2221at 2245.772 kb on + strand, within EX28DRAFT_2221at 2246.396 kb on + strand, within EX28DRAFT_2222at 2246.408 kb on - strand, within EX28DRAFT_2222at 2246.523 kb on + strand, within EX28DRAFT_2222at 2246.527 kb on + strand, within EX28DRAFT_2222at 2246.527 kb on + strand, within EX28DRAFT_2222at 2246.704 kb on - strand, within EX28DRAFT_2222at 2246.949 kb on + strand, within EX28DRAFT_2222at 2246.998 kb on + strand, within EX28DRAFT_2222at 2246.998 kb on + strand, within EX28DRAFT_2222at 2247.090 kb on - strand, within EX28DRAFT_2222at 2247.090 kb on - strand, within EX28DRAFT_2222at 2247.230 kb on - strandat 2247.230 kb on - strandat 2247.249 kb on + strandat 2247.258 kb on - strandat 2247.281 kb on + strandat 2247.641 kb on + strand, within EX28DRAFT_2223at 2247.648 kb on + strand, within EX28DRAFT_2223at 2247.649 kb on - strand, within EX28DRAFT_2223at 2247.672 kb on - strand, within EX28DRAFT_2223at 2247.822 kb on - strand, within EX28DRAFT_2223at 2247.823 kb on + strand, within EX28DRAFT_2223at 2247.823 kb on + strand, within EX28DRAFT_2223at 2247.851 kb on - strand, within EX28DRAFT_2223at 2247.876 kb on - strand, within EX28DRAFT_2223at 2248.054 kb on - strand, within EX28DRAFT_2223

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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2,245,397 + +0.1
2,245,449 + +0.1
2,245,449 + +2.7
2,245,450 - +0.1
2,245,450 - -0.2
2,245,450 - +0.4
2,245,508 + -0.3
2,245,532 - -0.6
2,245,538 + +0.1
2,245,660 - EX28DRAFT_2221 0.17 -0.5
2,245,748 + EX28DRAFT_2221 0.29 -0.7
2,245,749 - EX28DRAFT_2221 0.29 +0.3
2,245,749 - EX28DRAFT_2221 0.29 +0.8
2,245,772 + EX28DRAFT_2221 0.32 -0.1
2,245,772 + EX28DRAFT_2221 0.32 -0.1
2,246,396 + EX28DRAFT_2222 0.11 -1.0
2,246,408 - EX28DRAFT_2222 0.12 -0.7
2,246,523 + EX28DRAFT_2222 0.25 -0.7
2,246,527 + EX28DRAFT_2222 0.25 -0.8
2,246,527 + EX28DRAFT_2222 0.25 -1.9
2,246,704 - EX28DRAFT_2222 0.44 -0.2
2,246,949 + EX28DRAFT_2222 0.70 -0.7
2,246,998 + EX28DRAFT_2222 0.75 -0.5
2,246,998 + EX28DRAFT_2222 0.75 -1.0
2,247,090 - EX28DRAFT_2222 0.85 +0.1
2,247,090 - EX28DRAFT_2222 0.85 -0.4
2,247,230 - -0.3
2,247,230 - -0.4
2,247,249 + -0.7
2,247,258 - +0.7
2,247,281 + -0.8
2,247,641 + EX28DRAFT_2223 0.34 -1.3
2,247,648 + EX28DRAFT_2223 0.34 +0.4
2,247,649 - EX28DRAFT_2223 0.35 -0.0
2,247,672 - EX28DRAFT_2223 0.36 -1.2
2,247,822 - EX28DRAFT_2223 0.49 -0.3
2,247,823 + EX28DRAFT_2223 0.49 -0.7
2,247,823 + EX28DRAFT_2223 0.49 +0.4
2,247,851 - EX28DRAFT_2223 0.51 -0.2
2,247,876 - EX28DRAFT_2223 0.53 +1.2
2,248,054 - EX28DRAFT_2223 0.68 +0.3

Or see this region's nucleotide sequence