Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1373 and EX28DRAFT_1374 overlap by 4 nucleotides EX28DRAFT_1374 and EX28DRAFT_1375 overlap by 4 nucleotides EX28DRAFT_1375 and EX28DRAFT_1376 are separated by 256 nucleotides EX28DRAFT_1376 and EX28DRAFT_1377 are separated by 1 nucleotides
EX28DRAFT_1373: EX28DRAFT_1373 - Predicted NAD/FAD-binding protein, at 1,388,602 to 1,389,861
_1373
EX28DRAFT_1374: EX28DRAFT_1374 - Short-chain dehydrogenases of various substrate specificities, at 1,389,858 to 1,390,577
_1374
EX28DRAFT_1375: EX28DRAFT_1375 - SnoaL-like domain, at 1,390,574 to 1,391,008
_1375
EX28DRAFT_1376: EX28DRAFT_1376 - hypothetical protein, at 1,391,265 to 1,391,462
_1376
EX28DRAFT_1377: EX28DRAFT_1377 - hypothetical protein, at 1,391,464 to 1,392,252
_1377
Position (kb)
1390
1391
1392 Strain fitness (log2 ratio)
-1
0
1
2 at 1389.575 kb on + strand, within EX28DRAFT_1373 at 1389.714 kb on - strand, within EX28DRAFT_1373 at 1390.373 kb on - strand, within EX28DRAFT_1374 at 1390.635 kb on + strand, within EX28DRAFT_1375 at 1390.635 kb on + strand, within EX28DRAFT_1375 at 1390.636 kb on - strand, within EX28DRAFT_1375 at 1390.656 kb on + strand, within EX28DRAFT_1375 at 1390.656 kb on + strand, within EX28DRAFT_1375 at 1390.656 kb on + strand, within EX28DRAFT_1375 at 1390.656 kb on + strand, within EX28DRAFT_1375 at 1390.656 kb on + strand, within EX28DRAFT_1375 at 1390.659 kb on + strand, within EX28DRAFT_1375 at 1390.965 kb on + strand at 1390.966 kb on - strand at 1390.968 kb on + strand at 1390.968 kb on + strand at 1391.206 kb on + strand at 1391.308 kb on + strand, within EX28DRAFT_1376 at 1391.400 kb on + strand, within EX28DRAFT_1376 at 1391.463 kb on + strand at 1391.482 kb on - strand at 1391.482 kb on - strand at 1391.482 kb on - strand at 1391.509 kb on + strand at 1391.510 kb on - strand at 1391.510 kb on - strand at 1391.587 kb on + strand, within EX28DRAFT_1377 at 1391.587 kb on + strand, within EX28DRAFT_1377 at 1391.588 kb on - strand, within EX28DRAFT_1377 at 1391.695 kb on + strand, within EX28DRAFT_1377 at 1391.697 kb on + strand, within EX28DRAFT_1377 at 1391.718 kb on + strand, within EX28DRAFT_1377 at 1391.735 kb on + strand, within EX28DRAFT_1377 at 1391.799 kb on + strand, within EX28DRAFT_1377 at 1391.898 kb on + strand, within EX28DRAFT_1377 at 1391.898 kb on + strand, within EX28DRAFT_1377 at 1391.899 kb on - strand, within EX28DRAFT_1377 at 1391.925 kb on + strand, within EX28DRAFT_1377 at 1391.925 kb on + strand, within EX28DRAFT_1377 at 1391.948 kb on - strand, within EX28DRAFT_1377 at 1391.948 kb on - strand, within EX28DRAFT_1377
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,389,575 + EX28DRAFT_1373 0.77 +0.9 1,389,714 - EX28DRAFT_1373 0.88 +0.3 1,390,373 - EX28DRAFT_1374 0.72 +2.3 1,390,635 + EX28DRAFT_1375 0.14 -0.7 1,390,635 + EX28DRAFT_1375 0.14 +0.3 1,390,636 - EX28DRAFT_1375 0.14 -1.3 1,390,656 + EX28DRAFT_1375 0.19 +1.1 1,390,656 + EX28DRAFT_1375 0.19 +0.7 1,390,656 + EX28DRAFT_1375 0.19 +0.4 1,390,656 + EX28DRAFT_1375 0.19 +0.1 1,390,656 + EX28DRAFT_1375 0.19 -0.4 1,390,659 + EX28DRAFT_1375 0.20 -0.3 1,390,965 + +0.5 1,390,966 - -0.4 1,390,968 + +0.5 1,390,968 + -0.6 1,391,206 + +0.1 1,391,308 + EX28DRAFT_1376 0.22 +0.6 1,391,400 + EX28DRAFT_1376 0.68 -0.9 1,391,463 + -0.2 1,391,482 - +0.4 1,391,482 - -0.7 1,391,482 - +0.0 1,391,509 + -1.0 1,391,510 - +1.2 1,391,510 - +0.2 1,391,587 + EX28DRAFT_1377 0.16 -0.1 1,391,587 + EX28DRAFT_1377 0.16 -0.2 1,391,588 - EX28DRAFT_1377 0.16 -0.3 1,391,695 + EX28DRAFT_1377 0.29 +0.1 1,391,697 + EX28DRAFT_1377 0.30 +0.4 1,391,718 + EX28DRAFT_1377 0.32 +0.0 1,391,735 + EX28DRAFT_1377 0.34 +0.0 1,391,799 + EX28DRAFT_1377 0.42 +0.1 1,391,898 + EX28DRAFT_1377 0.55 +0.6 1,391,898 + EX28DRAFT_1377 0.55 +0.2 1,391,899 - EX28DRAFT_1377 0.55 +0.0 1,391,925 + EX28DRAFT_1377 0.58 -0.8 1,391,925 + EX28DRAFT_1377 0.58 +0.3 1,391,948 - EX28DRAFT_1377 0.61 +0.1 1,391,948 - EX28DRAFT_1377 0.61 -1.4
Or see this region's nucleotide sequence