Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_1252

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_1250 and EX28DRAFT_1251 are separated by 9 nucleotidesEX28DRAFT_1251 and EX28DRAFT_1252 are separated by 132 nucleotidesEX28DRAFT_1252 and EX28DRAFT_1253 are separated by 64 nucleotides EX28DRAFT_1250: EX28DRAFT_1250 - Protein of unknown function (DUF421), at 1,267,400 to 1,267,846 _1250 EX28DRAFT_1251: EX28DRAFT_1251 - hypothetical protein, at 1,267,856 to 1,268,365 _1251 EX28DRAFT_1252: EX28DRAFT_1252 - TIGR00156 family protein, at 1,268,498 to 1,268,887 _1252 EX28DRAFT_1253: EX28DRAFT_1253 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,268,952 to 1,269,836 _1253 Position (kb) 1268 1269Strain fitness (log2 ratio) -1 0 1at 1267.815 kb on + strandat 1267.820 kb on - strandat 1267.867 kb on + strandat 1267.990 kb on - strand, within EX28DRAFT_1251at 1268.023 kb on + strand, within EX28DRAFT_1251at 1268.024 kb on - strand, within EX28DRAFT_1251at 1268.024 kb on - strand, within EX28DRAFT_1251at 1268.094 kb on + strand, within EX28DRAFT_1251at 1268.094 kb on + strand, within EX28DRAFT_1251at 1268.094 kb on + strand, within EX28DRAFT_1251at 1268.094 kb on + strand, within EX28DRAFT_1251at 1268.095 kb on - strand, within EX28DRAFT_1251at 1268.095 kb on - strand, within EX28DRAFT_1251at 1268.095 kb on - strand, within EX28DRAFT_1251at 1268.095 kb on - strand, within EX28DRAFT_1251at 1268.166 kb on + strand, within EX28DRAFT_1251at 1268.167 kb on - strand, within EX28DRAFT_1251at 1268.171 kb on + strand, within EX28DRAFT_1251at 1268.172 kb on - strand, within EX28DRAFT_1251at 1268.272 kb on + strand, within EX28DRAFT_1251at 1268.442 kb on + strandat 1268.466 kb on + strandat 1268.781 kb on + strand, within EX28DRAFT_1252at 1268.951 kb on + strandat 1268.951 kb on + strandat 1268.964 kb on - strandat 1268.973 kb on - strandat 1268.973 kb on - strandat 1269.109 kb on + strand, within EX28DRAFT_1253at 1269.110 kb on - strand, within EX28DRAFT_1253at 1269.156 kb on + strand, within EX28DRAFT_1253at 1269.185 kb on + strand, within EX28DRAFT_1253at 1269.186 kb on - strand, within EX28DRAFT_1253at 1269.190 kb on - strand, within EX28DRAFT_1253at 1269.498 kb on - strand, within EX28DRAFT_1253at 1269.500 kb on + strand, within EX28DRAFT_1253at 1269.500 kb on + strand, within EX28DRAFT_1253at 1269.500 kb on + strand, within EX28DRAFT_1253at 1269.501 kb on - strand, within EX28DRAFT_1253at 1269.501 kb on - strand, within EX28DRAFT_1253at 1269.789 kb on - strandat 1269.835 kb on - strandat 1269.869 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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1,267,815 + +0.7
1,267,820 - -0.5
1,267,867 + -1.7
1,267,990 - EX28DRAFT_1251 0.26 -0.5
1,268,023 + EX28DRAFT_1251 0.33 +0.4
1,268,024 - EX28DRAFT_1251 0.33 -0.8
1,268,024 - EX28DRAFT_1251 0.33 -0.1
1,268,094 + EX28DRAFT_1251 0.47 -0.4
1,268,094 + EX28DRAFT_1251 0.47 +0.2
1,268,094 + EX28DRAFT_1251 0.47 +0.4
1,268,094 + EX28DRAFT_1251 0.47 -0.1
1,268,095 - EX28DRAFT_1251 0.47 +1.4
1,268,095 - EX28DRAFT_1251 0.47 +0.3
1,268,095 - EX28DRAFT_1251 0.47 -0.9
1,268,095 - EX28DRAFT_1251 0.47 -0.3
1,268,166 + EX28DRAFT_1251 0.61 +0.5
1,268,167 - EX28DRAFT_1251 0.61 +0.9
1,268,171 + EX28DRAFT_1251 0.62 -0.1
1,268,172 - EX28DRAFT_1251 0.62 +0.1
1,268,272 + EX28DRAFT_1251 0.82 -0.3
1,268,442 + -0.7
1,268,466 + +0.1
1,268,781 + EX28DRAFT_1252 0.73 +0.7
1,268,951 + +0.4
1,268,951 + +0.4
1,268,964 - +0.2
1,268,973 - -0.4
1,268,973 - +0.1
1,269,109 + EX28DRAFT_1253 0.18 -0.1
1,269,110 - EX28DRAFT_1253 0.18 +0.5
1,269,156 + EX28DRAFT_1253 0.23 +0.4
1,269,185 + EX28DRAFT_1253 0.26 -0.5
1,269,186 - EX28DRAFT_1253 0.26 +0.7
1,269,190 - EX28DRAFT_1253 0.27 +1.3
1,269,498 - EX28DRAFT_1253 0.62 -0.5
1,269,500 + EX28DRAFT_1253 0.62 -0.7
1,269,500 + EX28DRAFT_1253 0.62 +0.3
1,269,500 + EX28DRAFT_1253 0.62 +0.5
1,269,501 - EX28DRAFT_1253 0.62 -0.9
1,269,501 - EX28DRAFT_1253 0.62 +0.8
1,269,789 - +0.6
1,269,835 - -1.1
1,269,869 + +0.9

Or see this region's nucleotide sequence