Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1150 and EX28DRAFT_1151 overlap by 10 nucleotides EX28DRAFT_1151 and EX28DRAFT_1152 overlap by 53 nucleotides EX28DRAFT_1152 and EX28DRAFT_1153 are separated by 141 nucleotides EX28DRAFT_1153 and EX28DRAFT_1154 are separated by 77 nucleotides
EX28DRAFT_1150: EX28DRAFT_1150 - Transcriptional regulator, at 1,168,382 to 1,169,266
_1150
EX28DRAFT_1151: EX28DRAFT_1151 - Transcriptional regulator, at 1,169,257 to 1,170,222
_1151
EX28DRAFT_1152: EX28DRAFT_1152 - Metallo-beta-lactamase superfamily, at 1,170,170 to 1,171,156
_1152
EX28DRAFT_1153: EX28DRAFT_1153 - 5'-nucleotidase, lipoprotein e(P4) family, at 1,171,298 to 1,172,056
_1153
EX28DRAFT_1154: EX28DRAFT_1154 - Acetyltransferases, at 1,172,134 to 1,172,661
_1154
Position (kb)
1170
1171
1172 Strain fitness (log2 ratio)
-1
0
1 at 1169.340 kb on - strand at 1169.474 kb on + strand, within EX28DRAFT_1151 at 1169.474 kb on + strand, within EX28DRAFT_1151 at 1169.475 kb on - strand, within EX28DRAFT_1151 at 1169.714 kb on + strand, within EX28DRAFT_1151 at 1169.714 kb on + strand, within EX28DRAFT_1151 at 1169.715 kb on - strand, within EX28DRAFT_1151 at 1169.718 kb on + strand, within EX28DRAFT_1151 at 1170.142 kb on + strand at 1170.142 kb on + strand at 1170.143 kb on - strand at 1170.143 kb on - strand at 1170.143 kb on - strand at 1170.143 kb on - strand at 1170.143 kb on - strand at 1170.171 kb on + strand at 1170.172 kb on - strand at 1170.199 kb on + strand at 1170.200 kb on - strand at 1170.202 kb on - strand at 1170.202 kb on - strand at 1170.202 kb on - strand at 1170.202 kb on - strand at 1170.250 kb on - strand at 1170.250 kb on - strand at 1170.360 kb on + strand, within EX28DRAFT_1152 at 1170.559 kb on - strand, within EX28DRAFT_1152 at 1170.591 kb on + strand, within EX28DRAFT_1152 at 1170.751 kb on + strand, within EX28DRAFT_1152 at 1171.151 kb on - strand at 1171.156 kb on + strand at 1171.157 kb on - strand at 1171.184 kb on + strand at 1171.194 kb on + strand at 1171.592 kb on + strand, within EX28DRAFT_1153 at 1171.616 kb on - strand, within EX28DRAFT_1153 at 1171.644 kb on + strand, within EX28DRAFT_1153 at 1171.644 kb on + strand, within EX28DRAFT_1153 at 1171.645 kb on - strand, within EX28DRAFT_1153 at 1171.750 kb on + strand, within EX28DRAFT_1153 at 1171.750 kb on + strand, within EX28DRAFT_1153 at 1171.750 kb on + strand, within EX28DRAFT_1153 at 1171.751 kb on - strand, within EX28DRAFT_1153 at 1172.022 kb on - strand at 1172.147 kb on + strand at 1172.147 kb on + strand at 1172.147 kb on + strand at 1172.148 kb on - strand at 1172.148 kb on - strand at 1172.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,169,340 - +1.2 1,169,474 + EX28DRAFT_1151 0.22 -0.3 1,169,474 + EX28DRAFT_1151 0.22 +0.1 1,169,475 - EX28DRAFT_1151 0.23 -0.4 1,169,714 + EX28DRAFT_1151 0.47 -0.3 1,169,714 + EX28DRAFT_1151 0.47 +0.4 1,169,715 - EX28DRAFT_1151 0.47 +0.3 1,169,718 + EX28DRAFT_1151 0.48 -0.5 1,170,142 + -0.7 1,170,142 + +1.0 1,170,143 - +0.2 1,170,143 - +0.6 1,170,143 - -1.1 1,170,143 - -0.7 1,170,143 - -1.7 1,170,171 + -0.5 1,170,172 - +0.3 1,170,199 + -1.0 1,170,200 - +0.6 1,170,202 - -0.7 1,170,202 - -0.1 1,170,202 - -0.3 1,170,202 - +0.6 1,170,250 - +0.3 1,170,250 - +0.1 1,170,360 + EX28DRAFT_1152 0.19 -1.4 1,170,559 - EX28DRAFT_1152 0.39 -0.4 1,170,591 + EX28DRAFT_1152 0.43 -1.4 1,170,751 + EX28DRAFT_1152 0.59 -0.3 1,171,151 - +0.9 1,171,156 + +1.4 1,171,157 - -0.2 1,171,184 + -1.3 1,171,194 + -0.3 1,171,592 + EX28DRAFT_1153 0.39 -0.7 1,171,616 - EX28DRAFT_1153 0.42 +1.2 1,171,644 + EX28DRAFT_1153 0.46 -0.2 1,171,644 + EX28DRAFT_1153 0.46 +1.0 1,171,645 - EX28DRAFT_1153 0.46 +0.3 1,171,750 + EX28DRAFT_1153 0.60 +0.6 1,171,750 + EX28DRAFT_1153 0.60 +0.9 1,171,750 + EX28DRAFT_1153 0.60 -0.3 1,171,751 - EX28DRAFT_1153 0.60 -0.1 1,172,022 - -1.1 1,172,147 + -0.2 1,172,147 + -0.4 1,172,147 + -0.3 1,172,148 - +0.2 1,172,148 - +0.2 1,172,148 - +0.2
Or see this region's nucleotide sequence