Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0999 and EX28DRAFT_1000 are separated by 90 nucleotides EX28DRAFT_1000 and EX28DRAFT_1001 are separated by 432 nucleotides
EX28DRAFT_0999: EX28DRAFT_0999 - ABC-type oligopeptide transport system, periplasmic component, at 1,002,336 to 1,003,952
_0999
EX28DRAFT_1000: EX28DRAFT_1000 - intracellular protease, PfpI family, at 1,004,043 to 1,004,606
_1000
EX28DRAFT_1001: EX28DRAFT_1001 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 1,005,039 to 1,006,271
_1001
Position (kb)
1004
1005 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1003.100 kb on + strand, within EX28DRAFT_0999 at 1003.122 kb on - strand, within EX28DRAFT_0999 at 1003.155 kb on + strand, within EX28DRAFT_0999 at 1003.191 kb on + strand, within EX28DRAFT_0999 at 1003.193 kb on + strand, within EX28DRAFT_0999 at 1003.215 kb on + strand, within EX28DRAFT_0999 at 1003.215 kb on + strand, within EX28DRAFT_0999 at 1003.215 kb on + strand, within EX28DRAFT_0999 at 1003.215 kb on + strand, within EX28DRAFT_0999 at 1003.216 kb on - strand, within EX28DRAFT_0999 at 1003.216 kb on - strand, within EX28DRAFT_0999 at 1003.216 kb on - strand, within EX28DRAFT_0999 at 1003.218 kb on + strand, within EX28DRAFT_0999 at 1003.219 kb on - strand, within EX28DRAFT_0999 at 1003.219 kb on - strand, within EX28DRAFT_0999 at 1003.221 kb on + strand, within EX28DRAFT_0999 at 1003.221 kb on + strand, within EX28DRAFT_0999 at 1003.222 kb on - strand, within EX28DRAFT_0999 at 1003.222 kb on - strand, within EX28DRAFT_0999 at 1003.318 kb on + strand, within EX28DRAFT_0999 at 1003.318 kb on + strand, within EX28DRAFT_0999 at 1003.318 kb on + strand, within EX28DRAFT_0999 at 1003.319 kb on - strand, within EX28DRAFT_0999 at 1003.319 kb on - strand, within EX28DRAFT_0999 at 1003.319 kb on - strand, within EX28DRAFT_0999 at 1003.319 kb on - strand, within EX28DRAFT_0999 at 1003.322 kb on + strand, within EX28DRAFT_0999 at 1003.322 kb on + strand, within EX28DRAFT_0999 at 1003.492 kb on - strand, within EX28DRAFT_0999 at 1003.538 kb on + strand, within EX28DRAFT_0999 at 1003.539 kb on - strand, within EX28DRAFT_0999 at 1003.656 kb on + strand, within EX28DRAFT_0999 at 1003.657 kb on - strand, within EX28DRAFT_0999 at 1003.707 kb on - strand, within EX28DRAFT_0999 at 1003.709 kb on + strand, within EX28DRAFT_0999 at 1003.710 kb on - strand, within EX28DRAFT_0999 at 1003.845 kb on + strand at 1003.851 kb on + strand at 1003.851 kb on + strand at 1003.852 kb on - strand at 1003.935 kb on + strand at 1003.935 kb on + strand at 1003.935 kb on + strand at 1003.936 kb on - strand at 1003.936 kb on - strand at 1003.959 kb on - strand at 1003.995 kb on - strand at 1004.061 kb on - strand at 1004.474 kb on + strand, within EX28DRAFT_1000 at 1004.476 kb on + strand, within EX28DRAFT_1000 at 1004.477 kb on - strand, within EX28DRAFT_1000 at 1004.477 kb on - strand, within EX28DRAFT_1000 at 1004.572 kb on + strand at 1004.637 kb on - strand at 1004.641 kb on + strand at 1004.641 kb on + strand at 1004.641 kb on + strand at 1004.642 kb on - strand at 1004.642 kb on - strand at 1004.700 kb on + strand at 1004.700 kb on + strand at 1004.713 kb on - strand at 1004.781 kb on + strand at 1004.791 kb on - strand at 1004.936 kb on - strand at 1004.948 kb on - strand at 1004.974 kb on + strand at 1004.974 kb on + strand at 1005.011 kb on + strand at 1005.185 kb on + strand, within EX28DRAFT_1001 at 1005.186 kb on - strand, within EX28DRAFT_1001 at 1005.309 kb on - strand, within EX28DRAFT_1001 at 1005.375 kb on + strand, within EX28DRAFT_1001 at 1005.378 kb on - strand, within EX28DRAFT_1001 at 1005.378 kb on - strand, within EX28DRAFT_1001 at 1005.441 kb on - strand, within EX28DRAFT_1001 at 1005.511 kb on - strand, within EX28DRAFT_1001 at 1005.592 kb on + strand, within EX28DRAFT_1001 at 1005.593 kb on - strand, within EX28DRAFT_1001
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,003,100 + EX28DRAFT_0999 0.47 +0.9 1,003,122 - EX28DRAFT_0999 0.49 +0.1 1,003,155 + EX28DRAFT_0999 0.51 +0.9 1,003,191 + EX28DRAFT_0999 0.53 -1.5 1,003,193 + EX28DRAFT_0999 0.53 -2.3 1,003,215 + EX28DRAFT_0999 0.54 +0.8 1,003,215 + EX28DRAFT_0999 0.54 +0.2 1,003,215 + EX28DRAFT_0999 0.54 +0.1 1,003,215 + EX28DRAFT_0999 0.54 -0.1 1,003,216 - EX28DRAFT_0999 0.54 -0.1 1,003,216 - EX28DRAFT_0999 0.54 -0.7 1,003,216 - EX28DRAFT_0999 0.54 -0.3 1,003,218 + EX28DRAFT_0999 0.55 +0.0 1,003,219 - EX28DRAFT_0999 0.55 +0.0 1,003,219 - EX28DRAFT_0999 0.55 -2.6 1,003,221 + EX28DRAFT_0999 0.55 +0.1 1,003,221 + EX28DRAFT_0999 0.55 -0.7 1,003,222 - EX28DRAFT_0999 0.55 -2.3 1,003,222 - EX28DRAFT_0999 0.55 +0.5 1,003,318 + EX28DRAFT_0999 0.61 -0.2 1,003,318 + EX28DRAFT_0999 0.61 -0.3 1,003,318 + EX28DRAFT_0999 0.61 +2.0 1,003,319 - EX28DRAFT_0999 0.61 +0.2 1,003,319 - EX28DRAFT_0999 0.61 -0.2 1,003,319 - EX28DRAFT_0999 0.61 +0.5 1,003,319 - EX28DRAFT_0999 0.61 -1.2 1,003,322 + EX28DRAFT_0999 0.61 +0.5 1,003,322 + EX28DRAFT_0999 0.61 +1.0 1,003,492 - EX28DRAFT_0999 0.71 +0.8 1,003,538 + EX28DRAFT_0999 0.74 -1.9 1,003,539 - EX28DRAFT_0999 0.74 -0.1 1,003,656 + EX28DRAFT_0999 0.82 +0.3 1,003,657 - EX28DRAFT_0999 0.82 -0.3 1,003,707 - EX28DRAFT_0999 0.85 -0.3 1,003,709 + EX28DRAFT_0999 0.85 -0.0 1,003,710 - EX28DRAFT_0999 0.85 +0.5 1,003,845 + +0.0 1,003,851 + +0.0 1,003,851 + +0.7 1,003,852 - -1.6 1,003,935 + -0.4 1,003,935 + +0.3 1,003,935 + +0.2 1,003,936 - -0.6 1,003,936 - -0.2 1,003,959 - -0.4 1,003,995 - +0.2 1,004,061 - -1.3 1,004,474 + EX28DRAFT_1000 0.76 +0.3 1,004,476 + EX28DRAFT_1000 0.77 -1.9 1,004,477 - EX28DRAFT_1000 0.77 -2.0 1,004,477 - EX28DRAFT_1000 0.77 -0.2 1,004,572 + +0.3 1,004,637 - -0.5 1,004,641 + -0.2 1,004,641 + +0.2 1,004,641 + -1.0 1,004,642 - +0.3 1,004,642 - -0.4 1,004,700 + -0.5 1,004,700 + +1.0 1,004,713 - -0.3 1,004,781 + -0.2 1,004,791 - +0.9 1,004,936 - -0.7 1,004,948 - +0.1 1,004,974 + -0.2 1,004,974 + +0.6 1,005,011 + +1.0 1,005,185 + EX28DRAFT_1001 0.12 -0.6 1,005,186 - EX28DRAFT_1001 0.12 +1.9 1,005,309 - EX28DRAFT_1001 0.22 +0.4 1,005,375 + EX28DRAFT_1001 0.27 -0.5 1,005,378 - EX28DRAFT_1001 0.27 -0.3 1,005,378 - EX28DRAFT_1001 0.27 -0.5 1,005,441 - EX28DRAFT_1001 0.33 -0.2 1,005,511 - EX28DRAFT_1001 0.38 -0.0 1,005,592 + EX28DRAFT_1001 0.45 -1.3 1,005,593 - EX28DRAFT_1001 0.45 +1.7
Or see this region's nucleotide sequence