Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0931 and EX28DRAFT_0932 are separated by 133 nucleotides EX28DRAFT_0932 and EX28DRAFT_0933 overlap by 1 nucleotides
EX28DRAFT_0931: EX28DRAFT_0931 - Response regulator, at 929,913 to 930,548
_0931
EX28DRAFT_0932: EX28DRAFT_0932 - tryptophan synthase, alpha chain (EC 4.2.1.20), at 930,682 to 931,491
_0932
EX28DRAFT_0933: EX28DRAFT_0933 - tryptophan synthase, beta subunit, at 931,491 to 932,684
_0933
Position (kb) 930
931
932 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 929.709 kb on - strand at 929.709 kb on - strand at 929.811 kb on + strand at 929.827 kb on + strand at 929.878 kb on + strand at 929.883 kb on - strand at 929.955 kb on - strand at 929.962 kb on - strand at 930.061 kb on + strand, within EX28DRAFT_0931 at 930.062 kb on - strand, within EX28DRAFT_0931 at 930.062 kb on - strand, within EX28DRAFT_0931 at 930.064 kb on + strand, within EX28DRAFT_0931 at 930.107 kb on - strand, within EX28DRAFT_0931 at 930.181 kb on + strand, within EX28DRAFT_0931 at 930.242 kb on + strand, within EX28DRAFT_0931 at 930.455 kb on + strand, within EX28DRAFT_0931 at 930.505 kb on + strand at 930.546 kb on + strand at 930.546 kb on + strand at 930.556 kb on + strand at 930.598 kb on + strand at 930.598 kb on + strand at 930.603 kb on - strand at 930.603 kb on - strand at 930.684 kb on - strand at 930.968 kb on + strand, within EX28DRAFT_0932 at 930.969 kb on - strand, within EX28DRAFT_0932 at 930.972 kb on + strand, within EX28DRAFT_0932 at 930.973 kb on - strand, within EX28DRAFT_0932 at 930.975 kb on - strand, within EX28DRAFT_0932 at 931.149 kb on - strand, within EX28DRAFT_0932 at 931.149 kb on - strand, within EX28DRAFT_0932 at 931.161 kb on + strand, within EX28DRAFT_0932 at 931.471 kb on + strand at 931.472 kb on - strand at 931.472 kb on - strand at 931.535 kb on + strand at 931.536 kb on - strand at 931.714 kb on + strand, within EX28DRAFT_0933 at 931.715 kb on - strand, within EX28DRAFT_0933 at 931.792 kb on + strand, within EX28DRAFT_0933 at 931.792 kb on + strand, within EX28DRAFT_0933 at 931.793 kb on - strand, within EX28DRAFT_0933 at 931.849 kb on + strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.143 kb on + strand, within EX28DRAFT_0933 at 932.143 kb on + strand, within EX28DRAFT_0933 at 932.144 kb on - strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.288 kb on - strand, within EX28DRAFT_0933 at 932.427 kb on - strand, within EX28DRAFT_0933 at 932.464 kb on + strand, within EX28DRAFT_0933
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 929,709 - +0.3 929,709 - +0.3 929,811 + -0.2 929,827 + +0.0 929,878 + +1.5 929,883 - -0.0 929,955 - -0.5 929,962 - -1.1 930,061 + EX28DRAFT_0931 0.23 +0.4 930,062 - EX28DRAFT_0931 0.23 +0.1 930,062 - EX28DRAFT_0931 0.23 +0.1 930,064 + EX28DRAFT_0931 0.24 +2.0 930,107 - EX28DRAFT_0931 0.31 -0.2 930,181 + EX28DRAFT_0931 0.42 -0.2 930,242 + EX28DRAFT_0931 0.52 +2.3 930,455 + EX28DRAFT_0931 0.85 -1.0 930,505 + +0.6 930,546 + -0.9 930,546 + -0.3 930,556 + -0.3 930,598 + +0.5 930,598 + -0.0 930,603 - -0.1 930,603 - -0.4 930,684 - +0.3 930,968 + EX28DRAFT_0932 0.35 -3.5 930,969 - EX28DRAFT_0932 0.35 -2.3 930,972 + EX28DRAFT_0932 0.36 -2.6 930,973 - EX28DRAFT_0932 0.36 -4.4 930,975 - EX28DRAFT_0932 0.36 -2.9 931,149 - EX28DRAFT_0932 0.58 -2.5 931,149 - EX28DRAFT_0932 0.58 -3.1 931,161 + EX28DRAFT_0932 0.59 -2.0 931,471 + -3.5 931,472 - -2.6 931,472 - -2.2 931,535 + -1.3 931,536 - -2.8 931,714 + EX28DRAFT_0933 0.19 -4.2 931,715 - EX28DRAFT_0933 0.19 -2.2 931,792 + EX28DRAFT_0933 0.25 -2.9 931,792 + EX28DRAFT_0933 0.25 -3.6 931,793 - EX28DRAFT_0933 0.25 -4.6 931,849 + EX28DRAFT_0933 0.30 -4.6 932,127 - EX28DRAFT_0933 0.53 -1.3 932,127 - EX28DRAFT_0933 0.53 -1.3 932,127 - EX28DRAFT_0933 0.53 -4.1 932,128 + EX28DRAFT_0933 0.53 -1.4 932,128 + EX28DRAFT_0933 0.53 -4.3 932,128 + EX28DRAFT_0933 0.53 -4.0 932,129 - EX28DRAFT_0933 0.53 -2.3 932,129 - EX28DRAFT_0933 0.53 -3.2 932,129 - EX28DRAFT_0933 0.53 -4.3 932,129 - EX28DRAFT_0933 0.53 -4.6 932,129 - EX28DRAFT_0933 0.53 -3.2 932,129 - EX28DRAFT_0933 0.53 +0.7 932,143 + EX28DRAFT_0933 0.55 -3.1 932,143 + EX28DRAFT_0933 0.55 -5.4 932,144 - EX28DRAFT_0933 0.55 -3.4 932,287 + EX28DRAFT_0933 0.67 -3.5 932,287 + EX28DRAFT_0933 0.67 -3.4 932,287 + EX28DRAFT_0933 0.67 -4.1 932,288 - EX28DRAFT_0933 0.67 -4.0 932,427 - EX28DRAFT_0933 0.78 -2.9 932,464 + EX28DRAFT_0933 0.81 -2.7
Or see this region's nucleotide sequence