Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0924 and EX28DRAFT_0925 are separated by 56 nucleotides EX28DRAFT_0925 and EX28DRAFT_0926 are separated by 25 nucleotides EX28DRAFT_0926 and EX28DRAFT_0927 are separated by 282 nucleotides EX28DRAFT_0927 and EX28DRAFT_0928 are separated by 46 nucleotides
EX28DRAFT_0924: EX28DRAFT_0924 - intracellular septation protein A, at 924,656 to 925,195
_0924
EX28DRAFT_0925: EX28DRAFT_0925 - Uncharacterised protein family (UPF0259), at 925,252 to 925,995
_0925
EX28DRAFT_0926: EX28DRAFT_0926 - Predicted Fe-S-cluster oxidoreductase, at 926,021 to 926,425
_0926
EX28DRAFT_0927: EX28DRAFT_0927 - Outer membrane protein W, at 926,708 to 927,340
_0927
EX28DRAFT_0928: EX28DRAFT_0928 - hypothetical protein, at 927,387 to 927,728
_0928
Position (kb) 926
927 Strain fitness (log2 ratio) -1
0
1 at 925.215 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.271 kb on + strand at 925.271 kb on + strand at 925.272 kb on - strand at 925.272 kb on - strand at 925.540 kb on + strand, within EX28DRAFT_0925 at 925.541 kb on - strand, within EX28DRAFT_0925 at 925.541 kb on - strand, within EX28DRAFT_0925 at 925.564 kb on - strand, within EX28DRAFT_0925 at 925.615 kb on - strand, within EX28DRAFT_0925 at 925.909 kb on + strand, within EX28DRAFT_0925 at 925.910 kb on - strand, within EX28DRAFT_0925 at 925.910 kb on - strand, within EX28DRAFT_0925 at 925.910 kb on - strand, within EX28DRAFT_0925 at 925.910 kb on - strand, within EX28DRAFT_0925 at 925.910 kb on - strand, within EX28DRAFT_0925 at 925.990 kb on - strand at 926.090 kb on - strand, within EX28DRAFT_0926 at 926.417 kb on - strand at 926.479 kb on - strand at 926.650 kb on - strand at 926.650 kb on - strand at 926.653 kb on - strand at 926.666 kb on + strand at 926.736 kb on + strand at 926.736 kb on + strand at 926.739 kb on + strand at 926.788 kb on + strand, within EX28DRAFT_0927 at 926.807 kb on - strand, within EX28DRAFT_0927 at 926.857 kb on + strand, within EX28DRAFT_0927 at 926.857 kb on + strand, within EX28DRAFT_0927 at 926.857 kb on + strand, within EX28DRAFT_0927 at 926.880 kb on + strand, within EX28DRAFT_0927 at 926.897 kb on + strand, within EX28DRAFT_0927 at 926.897 kb on + strand, within EX28DRAFT_0927 at 926.897 kb on + strand, within EX28DRAFT_0927 at 926.897 kb on + strand, within EX28DRAFT_0927 at 926.898 kb on - strand, within EX28DRAFT_0927 at 926.898 kb on - strand, within EX28DRAFT_0927 at 926.900 kb on - strand, within EX28DRAFT_0927 at 927.059 kb on + strand, within EX28DRAFT_0927 at 927.059 kb on + strand, within EX28DRAFT_0927 at 927.061 kb on + strand, within EX28DRAFT_0927 at 927.231 kb on - strand, within EX28DRAFT_0927 at 927.252 kb on + strand, within EX28DRAFT_0927 at 927.252 kb on + strand, within EX28DRAFT_0927 at 927.289 kb on + strand at 927.290 kb on - strand at 927.309 kb on + strand at 927.309 kb on + strand at 927.313 kb on + strand at 927.313 kb on + strand at 927.314 kb on - strand at 927.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 925,215 - +0.3 925,265 - -0.0 925,265 - +0.2 925,265 - +0.7 925,270 - -0.8 925,270 - +0.2 925,270 - +0.1 925,270 - -1.1 925,270 - +0.5 925,271 + -1.2 925,271 + -0.1 925,272 - +0.2 925,272 - +1.0 925,540 + EX28DRAFT_0925 0.39 -1.3 925,541 - EX28DRAFT_0925 0.39 +0.5 925,541 - EX28DRAFT_0925 0.39 +0.1 925,564 - EX28DRAFT_0925 0.42 -0.1 925,615 - EX28DRAFT_0925 0.49 +0.1 925,909 + EX28DRAFT_0925 0.88 -0.2 925,910 - EX28DRAFT_0925 0.88 +0.6 925,910 - EX28DRAFT_0925 0.88 +0.0 925,910 - EX28DRAFT_0925 0.88 -0.5 925,910 - EX28DRAFT_0925 0.88 +0.1 925,910 - EX28DRAFT_0925 0.88 +0.8 925,990 - +0.3 926,090 - EX28DRAFT_0926 0.17 -1.1 926,417 - -0.2 926,479 - -0.2 926,650 - -0.5 926,650 - -0.6 926,653 - +0.8 926,666 + -0.3 926,736 + +0.1 926,736 + -0.4 926,739 + -0.7 926,788 + EX28DRAFT_0927 0.13 +1.0 926,807 - EX28DRAFT_0927 0.16 +0.9 926,857 + EX28DRAFT_0927 0.24 +0.1 926,857 + EX28DRAFT_0927 0.24 -0.2 926,857 + EX28DRAFT_0927 0.24 -0.1 926,880 + EX28DRAFT_0927 0.27 +0.0 926,897 + EX28DRAFT_0927 0.30 -0.3 926,897 + EX28DRAFT_0927 0.30 -0.1 926,897 + EX28DRAFT_0927 0.30 -0.5 926,897 + EX28DRAFT_0927 0.30 -0.1 926,898 - EX28DRAFT_0927 0.30 -0.5 926,898 - EX28DRAFT_0927 0.30 -0.5 926,900 - EX28DRAFT_0927 0.30 -0.8 927,059 + EX28DRAFT_0927 0.55 -0.1 927,059 + EX28DRAFT_0927 0.55 +0.2 927,061 + EX28DRAFT_0927 0.56 +1.1 927,231 - EX28DRAFT_0927 0.83 -0.3 927,252 + EX28DRAFT_0927 0.86 -0.5 927,252 + EX28DRAFT_0927 0.86 +0.1 927,289 + +0.3 927,290 - -0.5 927,309 + +0.0 927,309 + -0.7 927,313 + +0.1 927,313 + -0.3 927,314 - +1.7 927,314 - -0.4
Or see this region's nucleotide sequence