Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0729 and EX28DRAFT_0730 are separated by 68 nucleotides EX28DRAFT_0730 and EX28DRAFT_0731 are separated by 161 nucleotides EX28DRAFT_0731 and EX28DRAFT_0732 are separated by 74 nucleotides EX28DRAFT_0732 and EX28DRAFT_0733 are separated by 23 nucleotides
EX28DRAFT_0729: EX28DRAFT_0729 - transcriptional regulator, IclR family, at 728,900 to 729,691
_0729
EX28DRAFT_0730: EX28DRAFT_0730 - YobH-like protein, at 729,760 to 729,999
_0730
EX28DRAFT_0731: EX28DRAFT_0731 - MgrB protein, at 730,161 to 730,304
_0731
EX28DRAFT_0732: EX28DRAFT_0732 - YebO-like protein, at 730,379 to 730,669
_0732
EX28DRAFT_0733: EX28DRAFT_0733 - Predicted Zn-dependent hydrolases of the beta-lactamase fold, at 730,693 to 731,682
_0733
Position (kb) 729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 728.763 kb on + strand at 728.788 kb on + strand at 728.849 kb on - strand at 728.875 kb on + strand at 728.875 kb on + strand at 728.897 kb on + strand at 728.967 kb on + strand at 728.994 kb on + strand, within EX28DRAFT_0729 at 728.998 kb on + strand, within EX28DRAFT_0729 at 729.032 kb on - strand, within EX28DRAFT_0729 at 729.042 kb on + strand, within EX28DRAFT_0729 at 729.043 kb on - strand, within EX28DRAFT_0729 at 729.054 kb on + strand, within EX28DRAFT_0729 at 729.077 kb on + strand, within EX28DRAFT_0729 at 729.257 kb on + strand, within EX28DRAFT_0729 at 729.257 kb on + strand, within EX28DRAFT_0729 at 729.281 kb on + strand, within EX28DRAFT_0729 at 729.281 kb on + strand, within EX28DRAFT_0729 at 729.282 kb on - strand, within EX28DRAFT_0729 at 729.297 kb on - strand, within EX28DRAFT_0729 at 729.556 kb on - strand, within EX28DRAFT_0729 at 729.570 kb on + strand, within EX28DRAFT_0729 at 729.571 kb on - strand, within EX28DRAFT_0729 at 729.575 kb on - strand, within EX28DRAFT_0729 at 729.592 kb on - strand, within EX28DRAFT_0729 at 729.620 kb on + strand at 729.620 kb on + strand at 729.621 kb on - strand at 729.650 kb on - strand at 729.704 kb on + strand at 729.872 kb on - strand, within EX28DRAFT_0730 at 729.875 kb on + strand, within EX28DRAFT_0730 at 729.975 kb on + strand, within EX28DRAFT_0730 at 730.022 kb on + strand at 730.034 kb on - strand at 730.034 kb on - strand at 730.100 kb on - strand at 730.133 kb on + strand at 730.305 kb on + strand at 730.305 kb on + strand at 730.625 kb on - strand, within EX28DRAFT_0732 at 730.625 kb on - strand, within EX28DRAFT_0732 at 730.960 kb on - strand, within EX28DRAFT_0733 at 730.968 kb on + strand, within EX28DRAFT_0733
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 728,763 + -0.2 728,788 + +1.2 728,849 - +0.2 728,875 + +0.1 728,875 + +0.2 728,897 + -1.3 728,967 + -0.0 728,994 + EX28DRAFT_0729 0.12 -0.1 728,998 + EX28DRAFT_0729 0.12 -0.4 729,032 - EX28DRAFT_0729 0.17 -0.3 729,042 + EX28DRAFT_0729 0.18 -0.2 729,043 - EX28DRAFT_0729 0.18 -3.7 729,054 + EX28DRAFT_0729 0.19 -0.6 729,077 + EX28DRAFT_0729 0.22 +2.1 729,257 + EX28DRAFT_0729 0.45 -1.3 729,257 + EX28DRAFT_0729 0.45 -0.4 729,281 + EX28DRAFT_0729 0.48 +0.3 729,281 + EX28DRAFT_0729 0.48 +0.2 729,282 - EX28DRAFT_0729 0.48 -0.7 729,297 - EX28DRAFT_0729 0.50 +0.1 729,556 - EX28DRAFT_0729 0.83 -0.0 729,570 + EX28DRAFT_0729 0.85 -0.1 729,571 - EX28DRAFT_0729 0.85 +0.3 729,575 - EX28DRAFT_0729 0.85 -0.3 729,592 - EX28DRAFT_0729 0.87 -0.2 729,620 + -0.7 729,620 + -0.8 729,621 - -0.2 729,650 - -0.8 729,704 + +0.1 729,872 - EX28DRAFT_0730 0.47 -0.7 729,875 + EX28DRAFT_0730 0.48 -0.5 729,975 + EX28DRAFT_0730 0.90 -1.8 730,022 + +0.0 730,034 - -0.0 730,034 - +1.2 730,100 - -0.1 730,133 + -0.1 730,305 + +0.2 730,305 + +0.4 730,625 - EX28DRAFT_0732 0.85 +0.0 730,625 - EX28DRAFT_0732 0.85 +0.2 730,960 - EX28DRAFT_0733 0.27 -0.7 730,968 + EX28DRAFT_0733 0.28 +1.2
Or see this region's nucleotide sequence