Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0710 and EX28DRAFT_0711 are separated by 24 nucleotides EX28DRAFT_0711 and EX28DRAFT_0712 are separated by 110 nucleotides EX28DRAFT_0712 and EX28DRAFT_0713 are separated by 136 nucleotides EX28DRAFT_0713 and EX28DRAFT_0714 are separated by 1 nucleotides
EX28DRAFT_0710: EX28DRAFT_0710 - DNA polymerase III, epsilon subunit and related 3'-5' exonucleases, at 710,280 to 710,942
_0710
EX28DRAFT_0711: EX28DRAFT_0711 - Predicted amidohydrolase, at 710,967 to 711,617
_0711
EX28DRAFT_0712: EX28DRAFT_0712 - DNA polymerase III, theta subunit, at 711,728 to 711,958
_0712
EX28DRAFT_0713: EX28DRAFT_0713 - Uncharacterized protein, homolog of Cu resistance protein CopC, at 712,095 to 712,466
_0713
EX28DRAFT_0714: EX28DRAFT_0714 - Putative copper export protein, at 712,468 to 713,337
_0714
Position (kb) 711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 710.728 kb on + strand, within EX28DRAFT_0710 at 710.728 kb on + strand, within EX28DRAFT_0710 at 710.728 kb on + strand, within EX28DRAFT_0710 at 710.729 kb on - strand, within EX28DRAFT_0710 at 710.731 kb on + strand, within EX28DRAFT_0710 at 710.732 kb on - strand, within EX28DRAFT_0710 at 710.732 kb on - strand, within EX28DRAFT_0710 at 710.895 kb on + strand at 710.974 kb on - strand at 711.121 kb on + strand, within EX28DRAFT_0711 at 711.121 kb on + strand, within EX28DRAFT_0711 at 711.122 kb on - strand, within EX28DRAFT_0711 at 711.122 kb on - strand, within EX28DRAFT_0711 at 711.122 kb on - strand, within EX28DRAFT_0711 at 711.125 kb on + strand, within EX28DRAFT_0711 at 711.126 kb on - strand, within EX28DRAFT_0711 at 711.252 kb on - strand, within EX28DRAFT_0711 at 711.252 kb on - strand, within EX28DRAFT_0711 at 711.399 kb on + strand, within EX28DRAFT_0711 at 711.453 kb on + strand, within EX28DRAFT_0711 at 711.552 kb on - strand, within EX28DRAFT_0711 at 711.552 kb on - strand, within EX28DRAFT_0711 at 711.565 kb on - strand at 711.565 kb on - strand at 711.587 kb on - strand at 711.587 kb on - strand at 711.601 kb on - strand at 711.629 kb on + strand at 711.631 kb on + strand at 711.631 kb on + strand at 711.631 kb on + strand at 711.652 kb on - strand at 711.657 kb on + strand at 711.658 kb on - strand at 711.729 kb on + strand at 711.730 kb on - strand at 711.747 kb on + strand at 711.867 kb on + strand, within EX28DRAFT_0712 at 711.868 kb on - strand, within EX28DRAFT_0712 at 711.960 kb on - strand at 712.018 kb on - strand at 712.042 kb on + strand at 712.068 kb on - strand at 712.121 kb on - strand at 712.121 kb on - strand at 712.121 kb on - strand at 712.236 kb on - strand, within EX28DRAFT_0713 at 712.236 kb on - strand, within EX28DRAFT_0713 at 712.236 kb on - strand, within EX28DRAFT_0713 at 712.430 kb on + strand at 712.431 kb on - strand at 712.447 kb on - strand at 712.464 kb on + strand at 712.464 kb on + strand at 712.465 kb on - strand at 712.483 kb on + strand at 712.501 kb on - strand at 712.911 kb on + strand, within EX28DRAFT_0714 at 712.911 kb on + strand, within EX28DRAFT_0714 at 712.912 kb on - strand, within EX28DRAFT_0714 at 712.916 kb on - strand, within EX28DRAFT_0714
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 710,728 + EX28DRAFT_0710 0.68 +0.4 710,728 + EX28DRAFT_0710 0.68 -0.2 710,728 + EX28DRAFT_0710 0.68 -0.2 710,729 - EX28DRAFT_0710 0.68 -0.6 710,731 + EX28DRAFT_0710 0.68 -0.2 710,732 - EX28DRAFT_0710 0.68 +2.1 710,732 - EX28DRAFT_0710 0.68 +2.3 710,895 + -0.2 710,974 - -0.3 711,121 + EX28DRAFT_0711 0.24 +0.8 711,121 + EX28DRAFT_0711 0.24 -0.5 711,122 - EX28DRAFT_0711 0.24 -1.3 711,122 - EX28DRAFT_0711 0.24 -0.3 711,122 - EX28DRAFT_0711 0.24 -0.1 711,125 + EX28DRAFT_0711 0.24 +0.0 711,126 - EX28DRAFT_0711 0.24 +0.1 711,252 - EX28DRAFT_0711 0.44 -0.4 711,252 - EX28DRAFT_0711 0.44 -0.4 711,399 + EX28DRAFT_0711 0.66 +0.5 711,453 + EX28DRAFT_0711 0.75 +0.1 711,552 - EX28DRAFT_0711 0.90 -2.2 711,552 - EX28DRAFT_0711 0.90 -0.8 711,565 - +0.2 711,565 - +0.0 711,587 - +0.8 711,587 - +0.1 711,601 - +0.6 711,629 + +0.2 711,631 + -0.6 711,631 + -0.1 711,631 + -0.2 711,652 - -0.5 711,657 + +0.1 711,658 - -0.4 711,729 + -0.2 711,730 - +2.4 711,747 + +0.0 711,867 + EX28DRAFT_0712 0.60 -2.2 711,868 - EX28DRAFT_0712 0.61 -0.7 711,960 - -0.5 712,018 - -1.6 712,042 + +0.2 712,068 - -0.3 712,121 - -1.8 712,121 - -1.2 712,121 - +0.0 712,236 - EX28DRAFT_0713 0.38 +0.0 712,236 - EX28DRAFT_0713 0.38 -0.3 712,236 - EX28DRAFT_0713 0.38 -1.2 712,430 + +1.2 712,431 - +0.2 712,447 - +0.7 712,464 + -0.1 712,464 + +0.1 712,465 - -3.0 712,483 + +0.3 712,501 - -2.2 712,911 + EX28DRAFT_0714 0.51 +0.4 712,911 + EX28DRAFT_0714 0.51 -0.2 712,912 - EX28DRAFT_0714 0.51 -1.6 712,916 - EX28DRAFT_0714 0.51 +0.3
Or see this region's nucleotide sequence