Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0332 and EX28DRAFT_0333 are separated by 76 nucleotides EX28DRAFT_0333 and EX28DRAFT_0334 are separated by 6 nucleotides
EX28DRAFT_0332: EX28DRAFT_0332 - proton-translocating NADH-quinone oxidoreductase, chain L, at 292,647 to 294,488
_0332
EX28DRAFT_0333: EX28DRAFT_0333 - proton-translocating NADH-quinone oxidoreductase, chain M, at 294,565 to 296,094
_0333
EX28DRAFT_0334: EX28DRAFT_0334 - proton-translocating NADH-quinone oxidoreductase, chain N, at 296,101 to 297,558
_0334
Position (kb) 294
295
296
297 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 293.670 kb on - strand, within EX28DRAFT_0332 at 293.773 kb on - strand, within EX28DRAFT_0332 at 293.931 kb on - strand, within EX28DRAFT_0332 at 293.931 kb on - strand, within EX28DRAFT_0332 at 293.953 kb on + strand, within EX28DRAFT_0332 at 294.195 kb on - strand, within EX28DRAFT_0332 at 294.426 kb on + strand at 294.587 kb on + strand at 294.587 kb on + strand at 294.925 kb on - strand, within EX28DRAFT_0333 at 295.065 kb on + strand, within EX28DRAFT_0333 at 295.093 kb on + strand, within EX28DRAFT_0333 at 295.094 kb on - strand, within EX28DRAFT_0333 at 295.551 kb on + strand, within EX28DRAFT_0333 at 295.552 kb on - strand, within EX28DRAFT_0333 at 295.556 kb on - strand, within EX28DRAFT_0333 at 295.681 kb on - strand, within EX28DRAFT_0333 at 295.867 kb on + strand, within EX28DRAFT_0333 at 295.868 kb on - strand, within EX28DRAFT_0333 at 296.079 kb on + strand at 296.092 kb on + strand at 296.108 kb on + strand at 296.576 kb on - strand, within EX28DRAFT_0334 at 296.576 kb on - strand, within EX28DRAFT_0334 at 296.632 kb on + strand, within EX28DRAFT_0334
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 293,670 - EX28DRAFT_0332 0.56 -1.5 293,773 - EX28DRAFT_0332 0.61 -2.3 293,931 - EX28DRAFT_0332 0.70 -1.2 293,931 - EX28DRAFT_0332 0.70 -0.3 293,953 + EX28DRAFT_0332 0.71 -1.9 294,195 - EX28DRAFT_0332 0.84 +0.3 294,426 + -1.7 294,587 + -2.1 294,587 + -0.6 294,925 - EX28DRAFT_0333 0.24 +0.1 295,065 + EX28DRAFT_0333 0.33 -2.9 295,093 + EX28DRAFT_0333 0.35 -1.2 295,094 - EX28DRAFT_0333 0.35 -1.9 295,551 + EX28DRAFT_0333 0.64 -2.2 295,552 - EX28DRAFT_0333 0.65 -2.2 295,556 - EX28DRAFT_0333 0.65 -2.3 295,681 - EX28DRAFT_0333 0.73 -1.6 295,867 + EX28DRAFT_0333 0.85 -1.8 295,868 - EX28DRAFT_0333 0.85 -2.8 296,079 + -1.7 296,092 + -0.7 296,108 + -1.7 296,576 - EX28DRAFT_0334 0.33 -1.7 296,576 - EX28DRAFT_0334 0.33 -3.3 296,632 + EX28DRAFT_0334 0.36 -1.8
Or see this region's nucleotide sequence