Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0321 and EX28DRAFT_0322 are separated by 15 nucleotides EX28DRAFT_0322 and EX28DRAFT_0323 are separated by 89 nucleotides
EX28DRAFT_0321: EX28DRAFT_0321 - NADH:ubiquinone oxidoreductase subunit 3 (chain A), at 282,618 to 283,058
_0321
EX28DRAFT_0322: EX28DRAFT_0322 - NADH-quinone oxidoreductase, B subunit, at 283,074 to 283,748
_0322
EX28DRAFT_0323: EX28DRAFT_0323 - NADH (or F420H2) dehydrogenase, subunit C, at 283,838 to 285,640
_0323
Position (kb) 283
284 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 282.209 kb on + strand at 282.525 kb on - strand at 282.535 kb on + strand at 282.535 kb on + strand at 282.596 kb on + strand at 282.624 kb on - strand at 282.678 kb on - strand, within EX28DRAFT_0321 at 283.037 kb on + strand at 283.082 kb on + strand at 283.186 kb on - strand, within EX28DRAFT_0322 at 283.236 kb on - strand, within EX28DRAFT_0322 at 283.487 kb on + strand, within EX28DRAFT_0322 at 283.491 kb on + strand, within EX28DRAFT_0322 at 283.500 kb on + strand, within EX28DRAFT_0322 at 283.501 kb on - strand, within EX28DRAFT_0322 at 283.529 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.545 kb on + strand, within EX28DRAFT_0322 at 283.579 kb on - strand, within EX28DRAFT_0322 at 283.709 kb on + strand at 283.710 kb on - strand at 283.760 kb on - strand at 283.782 kb on + strand at 284.151 kb on + strand, within EX28DRAFT_0323 at 284.247 kb on - strand, within EX28DRAFT_0323 at 284.639 kb on + strand, within EX28DRAFT_0323 at 284.640 kb on - strand, within EX28DRAFT_0323
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 282,209 + +0.1 282,525 - -2.6 282,535 + -1.0 282,535 + -1.3 282,596 + -0.6 282,624 - -0.9 282,678 - EX28DRAFT_0321 0.14 -1.0 283,037 + -1.1 283,082 + -2.5 283,186 - EX28DRAFT_0322 0.17 -2.2 283,236 - EX28DRAFT_0322 0.24 -3.3 283,487 + EX28DRAFT_0322 0.61 -2.1 283,491 + EX28DRAFT_0322 0.62 -1.4 283,500 + EX28DRAFT_0322 0.63 -1.9 283,501 - EX28DRAFT_0322 0.63 -1.5 283,529 + EX28DRAFT_0322 0.67 -2.5 283,545 + EX28DRAFT_0322 0.70 -2.5 283,545 + EX28DRAFT_0322 0.70 -1.9 283,545 + EX28DRAFT_0322 0.70 -1.8 283,545 + EX28DRAFT_0322 0.70 -4.8 283,579 - EX28DRAFT_0322 0.75 -1.7 283,709 + -1.7 283,710 - -1.7 283,760 - -1.7 283,782 + -1.1 284,151 + EX28DRAFT_0323 0.17 -1.1 284,247 - EX28DRAFT_0323 0.23 +0.1 284,639 + EX28DRAFT_0323 0.44 -2.0 284,640 - EX28DRAFT_0323 0.44 -1.3
Or see this region's nucleotide sequence