Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0309 and EX28DRAFT_0310 overlap by 4 nucleotides EX28DRAFT_0310 and EX28DRAFT_0311 overlap by 8 nucleotides EX28DRAFT_0311 and EX28DRAFT_0312 are separated by 72 nucleotides
EX28DRAFT_0309: EX28DRAFT_0309 - Uncharacterized protein conserved in bacteria, at 267,504 to 268,895
_0309
EX28DRAFT_0310: EX28DRAFT_0310 - Transketolase, N-terminal subunit, at 268,892 to 269,722
_0310
EX28DRAFT_0311: EX28DRAFT_0311 - Transketolase, C-terminal subunit, at 269,715 to 270,668
_0311
EX28DRAFT_0312: EX28DRAFT_0312 - phosphate acetyltransferase, at 270,741 to 272,882
_0312
Position (kb) 269
270
271 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 268.909 kb on - strand at 268.987 kb on + strand, within EX28DRAFT_0310 at 268.988 kb on - strand, within EX28DRAFT_0310 at 268.988 kb on - strand, within EX28DRAFT_0310 at 269.111 kb on + strand, within EX28DRAFT_0310 at 269.112 kb on - strand, within EX28DRAFT_0310 at 269.371 kb on + strand, within EX28DRAFT_0310 at 269.770 kb on - strand at 269.903 kb on - strand, within EX28DRAFT_0311 at 269.966 kb on + strand, within EX28DRAFT_0311 at 269.967 kb on - strand, within EX28DRAFT_0311 at 270.011 kb on + strand, within EX28DRAFT_0311 at 270.011 kb on + strand, within EX28DRAFT_0311 at 270.024 kb on + strand, within EX28DRAFT_0311 at 270.046 kb on + strand, within EX28DRAFT_0311 at 270.047 kb on - strand, within EX28DRAFT_0311 at 270.095 kb on + strand, within EX28DRAFT_0311 at 270.096 kb on - strand, within EX28DRAFT_0311 at 270.213 kb on + strand, within EX28DRAFT_0311 at 270.312 kb on - strand, within EX28DRAFT_0311 at 270.314 kb on - strand, within EX28DRAFT_0311 at 270.314 kb on - strand, within EX28DRAFT_0311 at 270.447 kb on + strand, within EX28DRAFT_0311 at 270.491 kb on + strand, within EX28DRAFT_0311 at 270.492 kb on - strand, within EX28DRAFT_0311 at 270.742 kb on + strand at 270.742 kb on + strand at 271.081 kb on - strand, within EX28DRAFT_0312 at 271.484 kb on - strand, within EX28DRAFT_0312 at 271.619 kb on + strand, within EX28DRAFT_0312
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 268,909 - +0.4 268,987 + EX28DRAFT_0310 0.11 +0.1 268,988 - EX28DRAFT_0310 0.12 +0.3 268,988 - EX28DRAFT_0310 0.12 -0.1 269,111 + EX28DRAFT_0310 0.26 -0.0 269,112 - EX28DRAFT_0310 0.26 +0.5 269,371 + EX28DRAFT_0310 0.58 +0.2 269,770 - -0.5 269,903 - EX28DRAFT_0311 0.20 -0.1 269,966 + EX28DRAFT_0311 0.26 -0.8 269,967 - EX28DRAFT_0311 0.26 -0.4 270,011 + EX28DRAFT_0311 0.31 -1.2 270,011 + EX28DRAFT_0311 0.31 -0.4 270,024 + EX28DRAFT_0311 0.32 -1.7 270,046 + EX28DRAFT_0311 0.35 +1.1 270,047 - EX28DRAFT_0311 0.35 -0.1 270,095 + EX28DRAFT_0311 0.40 -0.0 270,096 - EX28DRAFT_0311 0.40 -0.1 270,213 + EX28DRAFT_0311 0.52 +0.1 270,312 - EX28DRAFT_0311 0.63 +0.6 270,314 - EX28DRAFT_0311 0.63 -0.1 270,314 - EX28DRAFT_0311 0.63 -4.8 270,447 + EX28DRAFT_0311 0.77 +0.7 270,491 + EX28DRAFT_0311 0.81 -0.1 270,492 - EX28DRAFT_0311 0.81 -0.3 270,742 + -0.0 270,742 + -0.5 271,081 - EX28DRAFT_0312 0.16 +0.5 271,484 - EX28DRAFT_0312 0.35 -1.3 271,619 + EX28DRAFT_0312 0.41 -1.1
Or see this region's nucleotide sequence