Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0290 and EX28DRAFT_0291 are separated by 229 nucleotides EX28DRAFT_0291 and EX28DRAFT_0292 are separated by 33 nucleotides EX28DRAFT_0292 and EX28DRAFT_0293 are separated by 89 nucleotides EX28DRAFT_0293 and EX28DRAFT_0294 are separated by 291 nucleotides
EX28DRAFT_0290: EX28DRAFT_0290 - Uncharacterized protein conserved in bacteria, at 252,869 to 253,561
_0290
EX28DRAFT_0291: EX28DRAFT_0291 - Uncharacterized membrane protein, required for colicin V production, at 253,791 to 254,279
_0291
EX28DRAFT_0292: EX28DRAFT_0292 - amidophosphoribosyltransferase (EC 2.4.2.14), at 254,313 to 255,830
_0292
EX28DRAFT_0293: EX28DRAFT_0293 - polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases, at 255,920 to 256,489
_0293
EX28DRAFT_0294: EX28DRAFT_0294 - lysine-arginine-ornithine-binding periplasmic protein, at 256,781 to 257,563
_0294
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 253.328 kb on + strand, within EX28DRAFT_0290 at 253.329 kb on - strand, within EX28DRAFT_0290 at 253.363 kb on + strand, within EX28DRAFT_0290 at 253.413 kb on - strand, within EX28DRAFT_0290 at 253.490 kb on - strand, within EX28DRAFT_0290 at 253.570 kb on + strand at 253.697 kb on + strand at 253.742 kb on + strand at 253.924 kb on - strand, within EX28DRAFT_0291 at 253.929 kb on + strand, within EX28DRAFT_0291 at 253.930 kb on - strand, within EX28DRAFT_0291 at 254.034 kb on + strand, within EX28DRAFT_0291 at 254.219 kb on + strand, within EX28DRAFT_0291 at 254.468 kb on - strand, within EX28DRAFT_0292 at 254.926 kb on - strand, within EX28DRAFT_0292 at 254.927 kb on + strand, within EX28DRAFT_0292 at 255.088 kb on - strand, within EX28DRAFT_0292 at 255.486 kb on + strand, within EX28DRAFT_0292 at 255.809 kb on - strand at 256.545 kb on - strand at 256.545 kb on - strand at 256.560 kb on + strand at 256.574 kb on - strand at 256.664 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.747 kb on - strand at 256.747 kb on - strand at 256.750 kb on + strand at 256.754 kb on - strand at 256.764 kb on + strand at 256.764 kb on + strand at 256.821 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 253,328 + EX28DRAFT_0290 0.66 +0.4 253,329 - EX28DRAFT_0290 0.66 +0.0 253,363 + EX28DRAFT_0290 0.71 +0.7 253,413 - EX28DRAFT_0290 0.78 +0.3 253,490 - EX28DRAFT_0290 0.90 +0.4 253,570 + -2.2 253,697 + +0.0 253,742 + -1.3 253,924 - EX28DRAFT_0291 0.27 -2.3 253,929 + EX28DRAFT_0291 0.28 -0.6 253,930 - EX28DRAFT_0291 0.28 -2.8 254,034 + EX28DRAFT_0291 0.50 -0.9 254,219 + EX28DRAFT_0291 0.88 -0.6 254,468 - EX28DRAFT_0292 0.10 -0.9 254,926 - EX28DRAFT_0292 0.40 -1.1 254,927 + EX28DRAFT_0292 0.40 -2.7 255,088 - EX28DRAFT_0292 0.51 -3.1 255,486 + EX28DRAFT_0292 0.77 -2.2 255,809 - +0.1 256,545 - -0.1 256,545 - -1.1 256,560 + -1.2 256,574 - +0.0 256,664 + -0.3 256,746 + +0.0 256,746 + -0.7 256,746 + -0.6 256,746 + -0.3 256,746 + -1.5 256,747 - +0.0 256,747 - -0.3 256,750 + +0.0 256,754 - +0.4 256,764 + +0.3 256,764 + +0.1 256,821 + -0.2
Or see this region's nucleotide sequence