Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0271 and EX28DRAFT_0272 are separated by 224 nucleotides EX28DRAFT_0272 and EX28DRAFT_0273 are separated by 46 nucleotides EX28DRAFT_0273 and EX28DRAFT_0274 are separated by 145 nucleotides
EX28DRAFT_0271: EX28DRAFT_0271 - fatty oxidation complex, alpha subunit FadJ, at 232,605 to 234,752
_0271
EX28DRAFT_0272: EX28DRAFT_0272 - phosphohistidine phosphatase, SixA, at 234,977 to 235,447
_0272
EX28DRAFT_0273: EX28DRAFT_0273 - Uncharacterized protein conserved in bacteria, at 235,494 to 236,045
_0273
EX28DRAFT_0274: EX28DRAFT_0274 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 236,191 to 237,123
_0274
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1
2 at 233.982 kb on - strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.084 kb on + strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.109 kb on - strand, within EX28DRAFT_0271 at 234.246 kb on - strand, within EX28DRAFT_0271 at 234.406 kb on + strand, within EX28DRAFT_0271 at 234.407 kb on - strand, within EX28DRAFT_0271 at 234.409 kb on - strand, within EX28DRAFT_0271 at 234.507 kb on - strand, within EX28DRAFT_0271 at 234.576 kb on + strand at 234.632 kb on + strand at 234.671 kb on + strand at 234.688 kb on + strand at 234.805 kb on - strand at 234.819 kb on + strand at 234.819 kb on + strand at 234.821 kb on + strand at 234.825 kb on + strand at 234.905 kb on + strand at 234.973 kb on + strand at 235.021 kb on - strand at 235.159 kb on + strand, within EX28DRAFT_0272 at 235.233 kb on - strand, within EX28DRAFT_0272 at 235.562 kb on - strand, within EX28DRAFT_0273 at 235.631 kb on + strand, within EX28DRAFT_0273 at 235.637 kb on + strand, within EX28DRAFT_0273 at 235.821 kb on + strand, within EX28DRAFT_0273 at 235.822 kb on - strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 236.152 kb on - strand at 236.197 kb on - strand at 236.201 kb on + strand at 236.233 kb on - strand at 236.237 kb on + strand at 236.238 kb on - strand at 236.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 233,982 - EX28DRAFT_0271 0.64 +1.8 234,084 + EX28DRAFT_0271 0.69 -0.1 234,084 + EX28DRAFT_0271 0.69 +0.1 234,084 + EX28DRAFT_0271 0.69 -0.2 234,109 - EX28DRAFT_0271 0.70 -0.3 234,109 - EX28DRAFT_0271 0.70 +0.1 234,109 - EX28DRAFT_0271 0.70 -0.3 234,246 - EX28DRAFT_0271 0.76 -0.1 234,406 + EX28DRAFT_0271 0.84 +0.6 234,407 - EX28DRAFT_0271 0.84 +1.7 234,409 - EX28DRAFT_0271 0.84 +1.2 234,507 - EX28DRAFT_0271 0.89 -0.6 234,576 + -0.0 234,632 + -0.1 234,671 + +0.3 234,688 + -0.1 234,805 - +0.4 234,819 + -0.3 234,819 + +0.0 234,821 + +0.1 234,825 + -0.1 234,905 + -0.2 234,973 + -1.5 235,021 - -1.0 235,159 + EX28DRAFT_0272 0.39 +0.1 235,233 - EX28DRAFT_0272 0.54 -1.9 235,562 - EX28DRAFT_0273 0.12 -1.2 235,631 + EX28DRAFT_0273 0.25 +0.5 235,637 + EX28DRAFT_0273 0.26 -0.4 235,821 + EX28DRAFT_0273 0.59 -0.4 235,822 - EX28DRAFT_0273 0.59 +0.4 235,825 + EX28DRAFT_0273 0.60 +0.4 235,825 + EX28DRAFT_0273 0.60 +0.3 235,825 + EX28DRAFT_0273 0.60 +0.0 235,826 - EX28DRAFT_0273 0.60 +0.2 235,826 - EX28DRAFT_0273 0.60 -0.9 235,920 + EX28DRAFT_0273 0.77 -0.1 235,920 + EX28DRAFT_0273 0.77 -0.2 236,152 - -0.4 236,197 - -0.9 236,201 + -0.1 236,233 - -0.5 236,237 + -0.4 236,238 - -2.5 236,284 - -0.3
Or see this region's nucleotide sequence