Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0173 and EX28DRAFT_0174 overlap by 1 nucleotides EX28DRAFT_0174 and EX28DRAFT_0175 are separated by 3 nucleotides EX28DRAFT_0175 and EX28DRAFT_0176 are separated by 146 nucleotides EX28DRAFT_0176 and EX28DRAFT_0177 overlap by 4 nucleotides
EX28DRAFT_0173: EX28DRAFT_0173 - Leucyl aminopeptidase, at 122,496 to 123,914
_0173
EX28DRAFT_0174: EX28DRAFT_0174 - Asparaginase, at 123,914 to 124,870
_0174
EX28DRAFT_0175: EX28DRAFT_0175 - phosphotransferase system, cellobiose specific, IIC component, at 124,874 to 126,193
_0175
EX28DRAFT_0176: EX28DRAFT_0176 - Homeodomain-like domain, at 126,340 to 127,164
_0176
EX28DRAFT_0177: EX28DRAFT_0177 - arsenate reductase (glutaredoxin), at 127,161 to 127,517
_0177
Position (kb) 124
125
126
127 Strain fitness (log2 ratio) -1
0
1
2 at 123.896 kb on + strand at 123.896 kb on + strand at 123.897 kb on - strand at 123.905 kb on - strand at 123.905 kb on - strand at 123.908 kb on + strand at 123.908 kb on + strand at 123.908 kb on + strand at 123.951 kb on + strand at 123.952 kb on - strand at 123.985 kb on - strand at 124.113 kb on - strand, within EX28DRAFT_0174 at 124.202 kb on - strand, within EX28DRAFT_0174 at 124.302 kb on + strand, within EX28DRAFT_0174 at 124.510 kb on + strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.511 kb on - strand, within EX28DRAFT_0174 at 124.578 kb on + strand, within EX28DRAFT_0174 at 124.660 kb on + strand, within EX28DRAFT_0174 at 124.735 kb on + strand, within EX28DRAFT_0174 at 124.875 kb on + strand at 124.876 kb on - strand at 124.952 kb on - strand at 124.957 kb on + strand at 124.958 kb on - strand at 124.958 kb on - strand at 124.996 kb on + strand at 125.072 kb on - strand, within EX28DRAFT_0175 at 125.119 kb on - strand, within EX28DRAFT_0175 at 125.218 kb on + strand, within EX28DRAFT_0175 at 125.356 kb on + strand, within EX28DRAFT_0175 at 125.515 kb on - strand, within EX28DRAFT_0175 at 125.557 kb on - strand, within EX28DRAFT_0175 at 125.557 kb on - strand, within EX28DRAFT_0175 at 125.580 kb on + strand, within EX28DRAFT_0175 at 125.580 kb on + strand, within EX28DRAFT_0175 at 125.581 kb on - strand, within EX28DRAFT_0175 at 126.016 kb on + strand, within EX28DRAFT_0175 at 126.016 kb on + strand, within EX28DRAFT_0175 at 126.028 kb on - strand, within EX28DRAFT_0175 at 126.099 kb on + strand at 126.232 kb on + strand at 126.499 kb on - strand, within EX28DRAFT_0176 at 126.499 kb on - strand, within EX28DRAFT_0176 at 126.743 kb on + strand, within EX28DRAFT_0176 at 126.743 kb on + strand, within EX28DRAFT_0176 at 126.744 kb on - strand, within EX28DRAFT_0176 at 126.768 kb on + strand, within EX28DRAFT_0176 at 126.769 kb on - strand, within EX28DRAFT_0176 at 126.769 kb on - strand, within EX28DRAFT_0176 at 127.162 kb on + strand at 127.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 123,896 + +0.8 123,896 + -1.5 123,897 - -0.5 123,905 - +0.2 123,905 - +0.8 123,908 + -0.2 123,908 + -0.2 123,908 + +0.5 123,951 + +0.9 123,952 - -0.9 123,985 - -0.7 124,113 - EX28DRAFT_0174 0.21 +0.2 124,202 - EX28DRAFT_0174 0.30 -0.3 124,302 + EX28DRAFT_0174 0.41 -0.0 124,510 + EX28DRAFT_0174 0.62 -0.4 124,511 - EX28DRAFT_0174 0.62 -0.7 124,511 - EX28DRAFT_0174 0.62 -0.2 124,511 - EX28DRAFT_0174 0.62 -0.0 124,511 - EX28DRAFT_0174 0.62 -0.0 124,578 + EX28DRAFT_0174 0.69 +1.9 124,660 + EX28DRAFT_0174 0.78 -0.9 124,735 + EX28DRAFT_0174 0.86 +0.2 124,875 + +0.3 124,876 - -0.3 124,952 - +0.2 124,957 + +0.1 124,958 - -1.0 124,958 - +0.1 124,996 + -0.1 125,072 - EX28DRAFT_0175 0.15 +0.4 125,119 - EX28DRAFT_0175 0.19 +0.1 125,218 + EX28DRAFT_0175 0.26 +1.0 125,356 + EX28DRAFT_0175 0.37 +0.1 125,515 - EX28DRAFT_0175 0.49 -0.2 125,557 - EX28DRAFT_0175 0.52 +0.5 125,557 - EX28DRAFT_0175 0.52 +0.2 125,580 + EX28DRAFT_0175 0.53 -0.1 125,580 + EX28DRAFT_0175 0.53 -0.3 125,581 - EX28DRAFT_0175 0.54 +0.1 126,016 + EX28DRAFT_0175 0.87 +0.4 126,016 + EX28DRAFT_0175 0.87 +0.6 126,028 - EX28DRAFT_0175 0.87 +0.2 126,099 + +1.5 126,232 + +0.4 126,499 - EX28DRAFT_0176 0.19 +2.6 126,499 - EX28DRAFT_0176 0.19 +0.0 126,743 + EX28DRAFT_0176 0.49 +0.1 126,743 + EX28DRAFT_0176 0.49 +0.6 126,744 - EX28DRAFT_0176 0.49 +0.1 126,768 + EX28DRAFT_0176 0.52 -0.9 126,769 - EX28DRAFT_0176 0.52 -0.0 126,769 - EX28DRAFT_0176 0.52 +0.8 127,162 + +0.0 127,162 + +2.1
Or see this region's nucleotide sequence