Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0171

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0169 and EX28DRAFT_0170 are separated by 227 nucleotidesEX28DRAFT_0170 and EX28DRAFT_0171 are separated by 83 nucleotidesEX28DRAFT_0171 and EX28DRAFT_0172 are separated by 81 nucleotidesEX28DRAFT_0172 and EX28DRAFT_0173 are separated by 41 nucleotides EX28DRAFT_0169: EX28DRAFT_0169 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 117,999 to 119,429 _0169 EX28DRAFT_0170: EX28DRAFT_0170 - uracil phosphoribosyltransferase (EC 2.4.2.9), at 119,657 to 120,283 _0170 EX28DRAFT_0171: EX28DRAFT_0171 - uracil-xanthine permease, at 120,367 to 121,656 _0171 EX28DRAFT_0172: EX28DRAFT_0172 - regulatory inactivation of DnaA Hda protein, at 121,738 to 122,454 _0172 EX28DRAFT_0173: EX28DRAFT_0173 - Leucyl aminopeptidase, at 122,496 to 123,914 _0173 Position (kb) 120 121 122Strain fitness (log2 ratio) -1 0 1 2at 119.393 kb on + strandat 119.394 kb on - strandat 119.394 kb on - strandat 119.394 kb on - strandat 119.427 kb on + strandat 119.465 kb on + strandat 119.466 kb on - strandat 119.466 kb on - strandat 119.466 kb on - strandat 119.471 kb on + strandat 119.471 kb on + strandat 119.481 kb on + strandat 119.482 kb on - strandat 119.567 kb on + strandat 119.568 kb on - strandat 119.568 kb on - strandat 119.568 kb on - strandat 119.568 kb on - strandat 119.592 kb on + strandat 119.960 kb on + strand, within EX28DRAFT_0170at 119.993 kb on + strand, within EX28DRAFT_0170at 119.994 kb on - strand, within EX28DRAFT_0170at 119.994 kb on - strand, within EX28DRAFT_0170at 120.110 kb on - strand, within EX28DRAFT_0170at 120.118 kb on + strand, within EX28DRAFT_0170at 120.119 kb on - strand, within EX28DRAFT_0170at 120.191 kb on + strand, within EX28DRAFT_0170at 120.192 kb on - strand, within EX28DRAFT_0170at 120.192 kb on - strand, within EX28DRAFT_0170at 120.192 kb on - strand, within EX28DRAFT_0170at 120.230 kb on + strandat 120.296 kb on - strandat 120.296 kb on - strandat 120.346 kb on + strandat 120.346 kb on + strandat 120.347 kb on - strandat 120.347 kb on - strandat 120.349 kb on + strandat 120.350 kb on - strandat 120.366 kb on + strandat 120.367 kb on - strandat 120.367 kb on - strandat 120.426 kb on + strandat 120.535 kb on + strand, within EX28DRAFT_0171at 120.591 kb on + strand, within EX28DRAFT_0171at 120.648 kb on + strand, within EX28DRAFT_0171at 120.649 kb on - strand, within EX28DRAFT_0171at 120.649 kb on - strand, within EX28DRAFT_0171at 120.792 kb on + strand, within EX28DRAFT_0171at 120.803 kb on + strand, within EX28DRAFT_0171at 120.804 kb on - strand, within EX28DRAFT_0171at 120.844 kb on - strand, within EX28DRAFT_0171at 121.021 kb on + strand, within EX28DRAFT_0171at 121.022 kb on - strand, within EX28DRAFT_0171at 121.023 kb on + strand, within EX28DRAFT_0171at 121.047 kb on + strand, within EX28DRAFT_0171at 121.125 kb on + strand, within EX28DRAFT_0171at 121.229 kb on - strand, within EX28DRAFT_0171at 121.229 kb on - strand, within EX28DRAFT_0171at 121.257 kb on + strand, within EX28DRAFT_0171at 121.268 kb on - strand, within EX28DRAFT_0171at 121.272 kb on + strand, within EX28DRAFT_0171at 121.273 kb on - strand, within EX28DRAFT_0171at 121.391 kb on - strand, within EX28DRAFT_0171at 121.445 kb on - strand, within EX28DRAFT_0171at 121.730 kb on + strandat 122.452 kb on + strandat 122.453 kb on - strandat 122.453 kb on - strandat 122.497 kb on + strandat 122.556 kb on - strandat 122.588 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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119,393 + +0.5
119,394 - +0.8
119,394 - +0.2
119,394 - +0.3
119,427 + +0.1
119,465 + +0.1
119,466 - +0.2
119,466 - -0.3
119,466 - -0.1
119,471 + -0.1
119,471 + +0.8
119,481 + +0.0
119,482 - +0.4
119,567 + +0.2
119,568 - -0.0
119,568 - +2.7
119,568 - +0.8
119,568 - +0.2
119,592 + +0.0
119,960 + EX28DRAFT_0170 0.48 +0.4
119,993 + EX28DRAFT_0170 0.54 -0.3
119,994 - EX28DRAFT_0170 0.54 -0.4
119,994 - EX28DRAFT_0170 0.54 +0.1
120,110 - EX28DRAFT_0170 0.72 +0.6
120,118 + EX28DRAFT_0170 0.74 -0.7
120,119 - EX28DRAFT_0170 0.74 -0.9
120,191 + EX28DRAFT_0170 0.85 -0.9
120,192 - EX28DRAFT_0170 0.85 -0.1
120,192 - EX28DRAFT_0170 0.85 +0.3
120,192 - EX28DRAFT_0170 0.85 +1.6
120,230 + -0.3
120,296 - -1.0
120,296 - +0.9
120,346 + +0.4
120,346 + -0.1
120,347 - -0.7
120,347 - +0.3
120,349 + +0.2
120,350 - +0.1
120,366 + -0.3
120,367 - +0.2
120,367 - +0.1
120,426 + -0.3
120,535 + EX28DRAFT_0171 0.13 +0.6
120,591 + EX28DRAFT_0171 0.17 +0.3
120,648 + EX28DRAFT_0171 0.22 +1.1
120,649 - EX28DRAFT_0171 0.22 +0.6
120,649 - EX28DRAFT_0171 0.22 +0.1
120,792 + EX28DRAFT_0171 0.33 +0.1
120,803 + EX28DRAFT_0171 0.34 -0.4
120,804 - EX28DRAFT_0171 0.34 -0.2
120,844 - EX28DRAFT_0171 0.37 +0.1
121,021 + EX28DRAFT_0171 0.51 -0.1
121,022 - EX28DRAFT_0171 0.51 -0.0
121,023 + EX28DRAFT_0171 0.51 -0.2
121,047 + EX28DRAFT_0171 0.53 +0.6
121,125 + EX28DRAFT_0171 0.59 -0.5
121,229 - EX28DRAFT_0171 0.67 +0.4
121,229 - EX28DRAFT_0171 0.67 +1.3
121,257 + EX28DRAFT_0171 0.69 -0.8
121,268 - EX28DRAFT_0171 0.70 +0.2
121,272 + EX28DRAFT_0171 0.70 +0.0
121,273 - EX28DRAFT_0171 0.70 +0.3
121,391 - EX28DRAFT_0171 0.79 +1.8
121,445 - EX28DRAFT_0171 0.84 +0.0
121,730 + +0.1
122,452 + +1.2
122,453 - +0.2
122,453 - -1.0
122,497 + +0.3
122,556 - +0.2
122,588 + -0.0

Or see this region's nucleotide sequence