Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0092 and EX28DRAFT_0093 are separated by 291 nucleotides EX28DRAFT_0093 and EX28DRAFT_0094 are separated by 96 nucleotides EX28DRAFT_0094 and EX28DRAFT_0095 are separated by 1 nucleotides EX28DRAFT_0095 and EX28DRAFT_0096 overlap by 14 nucleotides
EX28DRAFT_0092: EX28DRAFT_0092 - phosphoribosylformylglycinamidine synthase, single chain form, at 30,392 to 34,279
_0092
EX28DRAFT_0093: EX28DRAFT_0093 - RNA, at 34,571 to 34,717
_0093
EX28DRAFT_0094: EX28DRAFT_0094 - Signal transduction histidine kinase, at 34,814 to 36,241
_0094
EX28DRAFT_0095: EX28DRAFT_0095 - YfhG lipoprotein, at 36,243 to 36,980
_0095
EX28DRAFT_0096: EX28DRAFT_0096 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 36,967 to 38,304
_0096
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 34.409 kb on + strand at 34.444 kb on + strand at 34.445 kb on - strand at 34.458 kb on - strand at 34.590 kb on + strand, within EX28DRAFT_0093 at 34.591 kb on - strand, within EX28DRAFT_0093 at 34.591 kb on - strand, within EX28DRAFT_0093 at 34.615 kb on - strand, within EX28DRAFT_0093 at 34.618 kb on + strand, within EX28DRAFT_0093 at 34.619 kb on - strand, within EX28DRAFT_0093 at 34.659 kb on + strand, within EX28DRAFT_0093 at 34.659 kb on + strand, within EX28DRAFT_0093 at 34.660 kb on - strand, within EX28DRAFT_0093 at 34.660 kb on - strand, within EX28DRAFT_0093 at 34.679 kb on + strand, within EX28DRAFT_0093 at 34.705 kb on + strand at 34.756 kb on + strand at 34.808 kb on - strand at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.027 kb on + strand, within EX28DRAFT_0094 at 35.027 kb on + strand, within EX28DRAFT_0094 at 35.028 kb on - strand, within EX28DRAFT_0094 at 35.028 kb on - strand, within EX28DRAFT_0094 at 35.054 kb on - strand, within EX28DRAFT_0094 at 35.072 kb on + strand, within EX28DRAFT_0094 at 35.073 kb on - strand, within EX28DRAFT_0094 at 35.086 kb on + strand, within EX28DRAFT_0094 at 35.431 kb on + strand, within EX28DRAFT_0094 at 35.432 kb on - strand, within EX28DRAFT_0094 at 35.508 kb on + strand, within EX28DRAFT_0094 at 35.509 kb on - strand, within EX28DRAFT_0094 at 35.509 kb on - strand, within EX28DRAFT_0094 at 35.522 kb on - strand, within EX28DRAFT_0094 at 35.596 kb on + strand, within EX28DRAFT_0094 at 35.745 kb on - strand, within EX28DRAFT_0094 at 35.746 kb on + strand, within EX28DRAFT_0094 at 35.828 kb on - strand, within EX28DRAFT_0094 at 35.845 kb on + strand, within EX28DRAFT_0094 at 35.845 kb on + strand, within EX28DRAFT_0094 at 35.845 kb on + strand, within EX28DRAFT_0094 at 35.899 kb on + strand, within EX28DRAFT_0094 at 35.899 kb on + strand, within EX28DRAFT_0094 at 35.931 kb on - strand, within EX28DRAFT_0094 at 35.972 kb on + strand, within EX28DRAFT_0094 at 35.972 kb on + strand, within EX28DRAFT_0094 at 35.973 kb on - strand, within EX28DRAFT_0094 at 35.986 kb on + strand, within EX28DRAFT_0094 at 35.986 kb on + strand, within EX28DRAFT_0094 at 36.007 kb on + strand, within EX28DRAFT_0094 at 36.008 kb on - strand, within EX28DRAFT_0094 at 36.168 kb on + strand at 36.168 kb on + strand at 36.250 kb on + strand at 36.251 kb on - strand at 36.263 kb on + strand at 36.264 kb on - strand at 36.398 kb on + strand, within EX28DRAFT_0095 at 36.398 kb on + strand, within EX28DRAFT_0095 at 36.399 kb on - strand, within EX28DRAFT_0095 at 36.399 kb on - strand, within EX28DRAFT_0095 at 36.666 kb on + strand, within EX28DRAFT_0095 at 36.666 kb on + strand, within EX28DRAFT_0095 at 36.666 kb on + strand, within EX28DRAFT_0095 at 36.666 kb on + strand, within EX28DRAFT_0095 at 36.666 kb on + strand, within EX28DRAFT_0095 at 36.801 kb on - strand, within EX28DRAFT_0095 at 36.801 kb on - strand, within EX28DRAFT_0095 at 36.826 kb on + strand, within EX28DRAFT_0095 at 36.826 kb on + strand, within EX28DRAFT_0095 at 36.831 kb on - strand, within EX28DRAFT_0095 at 36.831 kb on - strand, within EX28DRAFT_0095 at 37.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 34,409 + -2.2 34,444 + +0.1 34,445 - +0.3 34,458 - -3.1 34,590 + EX28DRAFT_0093 0.13 +0.3 34,591 - EX28DRAFT_0093 0.14 -0.5 34,591 - EX28DRAFT_0093 0.14 -1.5 34,615 - EX28DRAFT_0093 0.30 -0.8 34,618 + EX28DRAFT_0093 0.32 -0.0 34,619 - EX28DRAFT_0093 0.33 +0.3 34,659 + EX28DRAFT_0093 0.60 -0.1 34,659 + EX28DRAFT_0093 0.60 +0.1 34,660 - EX28DRAFT_0093 0.61 -0.8 34,660 - EX28DRAFT_0093 0.61 -0.5 34,679 + EX28DRAFT_0093 0.73 +0.6 34,705 + +0.6 34,756 + +0.3 34,808 - -0.2 35,023 + EX28DRAFT_0094 0.15 -0.2 35,023 + EX28DRAFT_0094 0.15 -0.2 35,023 + EX28DRAFT_0094 0.15 -0.2 35,023 + EX28DRAFT_0094 0.15 -0.6 35,027 + EX28DRAFT_0094 0.15 -1.2 35,027 + EX28DRAFT_0094 0.15 -0.2 35,028 - EX28DRAFT_0094 0.15 -0.1 35,028 - EX28DRAFT_0094 0.15 +0.6 35,054 - EX28DRAFT_0094 0.17 -0.3 35,072 + EX28DRAFT_0094 0.18 -0.4 35,073 - EX28DRAFT_0094 0.18 -1.0 35,086 + EX28DRAFT_0094 0.19 +0.8 35,431 + EX28DRAFT_0094 0.43 -1.1 35,432 - EX28DRAFT_0094 0.43 -1.0 35,508 + EX28DRAFT_0094 0.49 -0.7 35,509 - EX28DRAFT_0094 0.49 -0.2 35,509 - EX28DRAFT_0094 0.49 -0.1 35,522 - EX28DRAFT_0094 0.50 +0.3 35,596 + EX28DRAFT_0094 0.55 -0.5 35,745 - EX28DRAFT_0094 0.65 +0.1 35,746 + EX28DRAFT_0094 0.65 -0.0 35,828 - EX28DRAFT_0094 0.71 -0.5 35,845 + EX28DRAFT_0094 0.72 -0.1 35,845 + EX28DRAFT_0094 0.72 -0.3 35,845 + EX28DRAFT_0094 0.72 +0.3 35,899 + EX28DRAFT_0094 0.76 -0.1 35,899 + EX28DRAFT_0094 0.76 -2.3 35,931 - EX28DRAFT_0094 0.78 -0.4 35,972 + EX28DRAFT_0094 0.81 -0.1 35,972 + EX28DRAFT_0094 0.81 -0.0 35,973 - EX28DRAFT_0094 0.81 -0.7 35,986 + EX28DRAFT_0094 0.82 -0.1 35,986 + EX28DRAFT_0094 0.82 -0.0 36,007 + EX28DRAFT_0094 0.84 +0.2 36,008 - EX28DRAFT_0094 0.84 -0.4 36,168 + +0.3 36,168 + +0.1 36,250 + +0.5 36,251 - -2.3 36,263 + -0.8 36,264 - -0.2 36,398 + EX28DRAFT_0095 0.21 -0.2 36,398 + EX28DRAFT_0095 0.21 -0.2 36,399 - EX28DRAFT_0095 0.21 -0.8 36,399 - EX28DRAFT_0095 0.21 -0.6 36,666 + EX28DRAFT_0095 0.57 +0.5 36,666 + EX28DRAFT_0095 0.57 -0.0 36,666 + EX28DRAFT_0095 0.57 -0.1 36,666 + EX28DRAFT_0095 0.57 +0.1 36,666 + EX28DRAFT_0095 0.57 -0.8 36,801 - EX28DRAFT_0095 0.76 +0.1 36,801 - EX28DRAFT_0095 0.76 -0.2 36,826 + EX28DRAFT_0095 0.79 -0.4 36,826 + EX28DRAFT_0095 0.79 +0.1 36,831 - EX28DRAFT_0095 0.80 +0.1 36,831 - EX28DRAFT_0095 0.80 -0.4 37,023 - -0.6
Or see this region's nucleotide sequence