Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07665 and AMB_RS24265 are separated by 11 nucleotides AMB_RS24265 and AMB_RS24270 overlap by 4 nucleotides AMB_RS24270 and AMB_RS07680 overlap by 391 nucleotides
AMB_RS07665: AMB_RS07665 - bacteriophage tail assembly protein, at 1,650,869 to 1,652,590
_RS07665
AMB_RS24265: AMB_RS24265 - hypothetical protein, at 1,652,602 to 1,652,805
_RS24265
AMB_RS24270: AMB_RS24270 - phage portal protein, at 1,652,802 to 1,654,313
_RS24270
AMB_RS07680: AMB_RS07680 - hypothetical protein, at 1,653,923 to 1,655,836
_RS07680
Position (kb)
1652
1653
1654
1655 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1651.828 kb on - strand, within AMB_RS07665 at 1651.917 kb on - strand, within AMB_RS07665 at 1652.094 kb on - strand, within AMB_RS07665 at 1652.162 kb on + strand, within AMB_RS07665 at 1652.163 kb on - strand, within AMB_RS07665 at 1652.187 kb on - strand, within AMB_RS07665 at 1652.420 kb on + strand at 1652.420 kb on + strand at 1652.420 kb on + strand at 1652.420 kb on + strand at 1652.421 kb on - strand at 1652.421 kb on - strand at 1652.574 kb on + strand at 1652.574 kb on + strand at 1652.574 kb on + strand at 1652.576 kb on + strand at 1652.576 kb on + strand at 1652.577 kb on - strand at 1652.577 kb on - strand at 1652.692 kb on + strand, within AMB_RS24265 at 1652.693 kb on - strand, within AMB_RS24265 at 1652.744 kb on + strand, within AMB_RS24265 at 1652.745 kb on - strand, within AMB_RS24265 at 1652.895 kb on + strand at 1652.904 kb on + strand at 1652.904 kb on + strand at 1652.904 kb on + strand at 1652.905 kb on - strand at 1652.991 kb on - strand, within AMB_RS24270 at 1653.023 kb on + strand, within AMB_RS24270 at 1653.065 kb on + strand, within AMB_RS24270 at 1653.065 kb on + strand, within AMB_RS24270 at 1653.128 kb on + strand, within AMB_RS24270 at 1653.312 kb on + strand, within AMB_RS24270 at 1653.313 kb on - strand, within AMB_RS24270 at 1653.321 kb on - strand, within AMB_RS24270 at 1653.359 kb on + strand, within AMB_RS24270 at 1653.375 kb on + strand, within AMB_RS24270 at 1653.473 kb on + strand, within AMB_RS24270 at 1653.474 kb on - strand, within AMB_RS24270 at 1653.579 kb on + strand, within AMB_RS24270 at 1653.580 kb on - strand, within AMB_RS24270 at 1654.049 kb on + strand at 1654.050 kb on - strand at 1654.050 kb on - strand at 1654.050 kb on - strand at 1654.053 kb on - strand at 1654.053 kb on - strand at 1654.161 kb on - strand at 1654.282 kb on + strand at 1654.370 kb on + strand, within AMB_RS07680 at 1654.370 kb on + strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.371 kb on - strand, within AMB_RS07680 at 1654.403 kb on + strand, within AMB_RS07680 at 1654.403 kb on + strand, within AMB_RS07680 at 1654.403 kb on + strand, within AMB_RS07680 at 1654.403 kb on + strand, within AMB_RS07680 at 1654.404 kb on - strand, within AMB_RS07680 at 1654.404 kb on - strand, within AMB_RS07680 at 1654.408 kb on + strand, within AMB_RS07680 at 1654.531 kb on + strand, within AMB_RS07680 at 1654.556 kb on + strand, within AMB_RS07680 at 1654.557 kb on - strand, within AMB_RS07680 at 1654.557 kb on - strand, within AMB_RS07680 at 1654.557 kb on - strand, within AMB_RS07680 at 1654.557 kb on - strand, within AMB_RS07680 at 1654.557 kb on - strand, within AMB_RS07680 at 1654.577 kb on + strand, within AMB_RS07680 at 1654.577 kb on + strand, within AMB_RS07680 at 1654.577 kb on + strand, within AMB_RS07680 at 1654.578 kb on - strand, within AMB_RS07680 at 1654.682 kb on + strand, within AMB_RS07680 at 1654.682 kb on + strand, within AMB_RS07680 at 1654.787 kb on + strand, within AMB_RS07680 at 1654.787 kb on + strand, within AMB_RS07680 at 1654.787 kb on + strand, within AMB_RS07680 at 1654.788 kb on - strand, within AMB_RS07680 at 1654.788 kb on - strand, within AMB_RS07680 at 1654.788 kb on - strand, within AMB_RS07680 at 1654.854 kb on - strand, within AMB_RS07680 at 1654.854 kb on - strand, within AMB_RS07680 at 1654.854 kb on - strand, within AMB_RS07680 at 1655.057 kb on + strand, within AMB_RS07680 at 1655.057 kb on + strand, within AMB_RS07680 at 1655.057 kb on + strand, within AMB_RS07680 at 1655.058 kb on - strand, within AMB_RS07680 at 1655.138 kb on + strand, within AMB_RS07680 at 1655.138 kb on + strand, within AMB_RS07680 at 1655.183 kb on + strand, within AMB_RS07680 at 1655.183 kb on + strand, within AMB_RS07680 at 1655.184 kb on - strand, within AMB_RS07680 at 1655.306 kb on + strand, within AMB_RS07680 at 1655.307 kb on - strand, within AMB_RS07680 at 1655.307 kb on - strand, within AMB_RS07680 at 1655.307 kb on - strand, within AMB_RS07680
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 1,651,828 - AMB_RS07665 0.56 +0.9 1,651,917 - AMB_RS07665 0.61 -0.4 1,652,094 - AMB_RS07665 0.71 -0.8 1,652,162 + AMB_RS07665 0.75 -0.2 1,652,163 - AMB_RS07665 0.75 -0.6 1,652,187 - AMB_RS07665 0.77 -1.8 1,652,420 + -0.6 1,652,420 + +0.5 1,652,420 + +0.3 1,652,420 + -1.7 1,652,421 - -0.1 1,652,421 - +0.9 1,652,574 + -3.8 1,652,574 + -0.7 1,652,574 + -0.8 1,652,576 + +0.3 1,652,576 + +0.9 1,652,577 - -0.6 1,652,577 - -1.2 1,652,692 + AMB_RS24265 0.44 +0.4 1,652,693 - AMB_RS24265 0.45 -1.5 1,652,744 + AMB_RS24265 0.70 -0.2 1,652,745 - AMB_RS24265 0.70 -1.7 1,652,895 + -1.3 1,652,904 + -0.6 1,652,904 + +0.4 1,652,904 + -2.7 1,652,905 - +0.1 1,652,991 - AMB_RS24270 0.12 +0.5 1,653,023 + AMB_RS24270 0.15 -0.3 1,653,065 + AMB_RS24270 0.17 +1.6 1,653,065 + AMB_RS24270 0.17 -1.1 1,653,128 + AMB_RS24270 0.22 +0.8 1,653,312 + AMB_RS24270 0.34 -0.4 1,653,313 - AMB_RS24270 0.34 -1.0 1,653,321 - AMB_RS24270 0.34 -0.5 1,653,359 + AMB_RS24270 0.37 +0.3 1,653,375 + AMB_RS24270 0.38 +1.2 1,653,473 + AMB_RS24270 0.44 -0.2 1,653,474 - AMB_RS24270 0.44 -1.1 1,653,579 + AMB_RS24270 0.51 -1.4 1,653,580 - AMB_RS24270 0.51 -0.8 1,654,049 + -0.1 1,654,050 - -0.1 1,654,050 - +0.7 1,654,050 - +0.7 1,654,053 - -1.7 1,654,053 - -1.6 1,654,161 - -0.4 1,654,282 + -0.1 1,654,370 + AMB_RS07680 0.23 +0.2 1,654,370 + AMB_RS07680 0.23 -1.0 1,654,371 - AMB_RS07680 0.23 +0.2 1,654,371 - AMB_RS07680 0.23 -1.7 1,654,371 - AMB_RS07680 0.23 -0.4 1,654,371 - AMB_RS07680 0.23 -0.5 1,654,371 - AMB_RS07680 0.23 -0.0 1,654,371 - AMB_RS07680 0.23 -0.6 1,654,403 + AMB_RS07680 0.25 +0.7 1,654,403 + AMB_RS07680 0.25 -0.2 1,654,403 + AMB_RS07680 0.25 +0.3 1,654,403 + AMB_RS07680 0.25 -1.8 1,654,404 - AMB_RS07680 0.25 -0.3 1,654,404 - AMB_RS07680 0.25 +1.5 1,654,408 + AMB_RS07680 0.25 -0.5 1,654,531 + AMB_RS07680 0.32 -0.7 1,654,556 + AMB_RS07680 0.33 +0.0 1,654,557 - AMB_RS07680 0.33 -0.4 1,654,557 - AMB_RS07680 0.33 -0.8 1,654,557 - AMB_RS07680 0.33 +0.1 1,654,557 - AMB_RS07680 0.33 -2.7 1,654,557 - AMB_RS07680 0.33 -1.7 1,654,577 + AMB_RS07680 0.34 -0.3 1,654,577 + AMB_RS07680 0.34 +0.3 1,654,577 + AMB_RS07680 0.34 +1.5 1,654,578 - AMB_RS07680 0.34 +0.3 1,654,682 + AMB_RS07680 0.40 +0.6 1,654,682 + AMB_RS07680 0.40 -0.0 1,654,787 + AMB_RS07680 0.45 +0.2 1,654,787 + AMB_RS07680 0.45 -1.1 1,654,787 + AMB_RS07680 0.45 +0.4 1,654,788 - AMB_RS07680 0.45 +0.1 1,654,788 - AMB_RS07680 0.45 -0.4 1,654,788 - AMB_RS07680 0.45 -0.6 1,654,854 - AMB_RS07680 0.49 +0.0 1,654,854 - AMB_RS07680 0.49 +1.5 1,654,854 - AMB_RS07680 0.49 +0.2 1,655,057 + AMB_RS07680 0.59 +0.3 1,655,057 + AMB_RS07680 0.59 +0.3 1,655,057 + AMB_RS07680 0.59 -1.6 1,655,058 - AMB_RS07680 0.59 -0.4 1,655,138 + AMB_RS07680 0.63 -0.3 1,655,138 + AMB_RS07680 0.63 -0.9 1,655,183 + AMB_RS07680 0.66 +0.1 1,655,183 + AMB_RS07680 0.66 -0.2 1,655,184 - AMB_RS07680 0.66 +0.2 1,655,306 + AMB_RS07680 0.72 -0.6 1,655,307 - AMB_RS07680 0.72 +0.5 1,655,307 - AMB_RS07680 0.72 -0.3 1,655,307 - AMB_RS07680 0.72 +0.7
Or see this region's nucleotide sequence