Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20570 and AMB_RS20575 are separated by 185 nucleotides AMB_RS20575 and AMB_RS20580 are separated by 36 nucleotides AMB_RS20580 and AMB_RS20585 are separated by 27 nucleotides AMB_RS20585 and AMB_RS20590 are separated by 10 nucleotides
AMB_RS20570: AMB_RS20570 - 3-isopropylmalate dehydrogenase, at 4,469,458 to 4,470,567
_RS20570
AMB_RS20575: AMB_RS20575 - ribosome-binding factor A, at 4,470,753 to 4,471,181
_RS20575
AMB_RS20580: AMB_RS20580 - translation initiation factor IF-2, at 4,471,218 to 4,473,836
_RS20580
AMB_RS20585: AMB_RS20585 - DNA-binding protein, at 4,473,864 to 4,474,460
_RS20585
AMB_RS20590: AMB_RS20590 - transcription termination/antitermination protein NusA, at 4,474,471 to 4,475,982
_RS20590
Position (kb) 4471
4472
4473
4474 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4470.754 kb on + strand at 4470.754 kb on + strand at 4471.219 kb on + strand at 4474.270 kb on - strand, within AMB_RS20585 at 4474.270 kb on - strand, within AMB_RS20585 at 4474.340 kb on + strand, within AMB_RS20585 at 4474.341 kb on - strand, within AMB_RS20585 at 4474.473 kb on - strand at 4474.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 4,470,754 + -1.8 4,470,754 + -0.9 4,471,219 + -2.8 4,474,270 - AMB_RS20585 0.68 -0.6 4,474,270 - AMB_RS20585 0.68 -1.2 4,474,340 + AMB_RS20585 0.80 -1.4 4,474,341 - AMB_RS20585 0.80 -1.3 4,474,473 - -2.5 4,474,473 - +0.4
Or see this region's nucleotide sequence