Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18040 and AMB_RS18045 are separated by 16 nucleotides AMB_RS18045 and AMB_RS18050 are separated by 90 nucleotides AMB_RS18050 and AMB_RS18055 are separated by 188 nucleotides
AMB_RS18040: AMB_RS18040 - sulfate ABC transporter permease subunit CysW, at 3,919,793 to 3,920,653
_RS18040
AMB_RS18045: AMB_RS18045 - sulfate ABC transporter permease subunit CysT, at 3,920,670 to 3,921,495
_RS18045
AMB_RS18050: AMB_RS18050 - sulfate ABC transporter substrate-binding protein, at 3,921,586 to 3,922,602
_RS18050
AMB_RS18055: AMB_RS18055 - GGDEF domain-containing protein, at 3,922,791 to 3,923,837
_RS18055
Position (kb)
3921
3922
3923 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3920.687 kb on - strand at 3920.687 kb on - strand at 3920.785 kb on + strand, within AMB_RS18045 at 3920.785 kb on + strand, within AMB_RS18045 at 3920.786 kb on - strand, within AMB_RS18045 at 3920.786 kb on - strand, within AMB_RS18045 at 3921.242 kb on + strand, within AMB_RS18045 at 3921.326 kb on + strand, within AMB_RS18045 at 3921.437 kb on + strand at 3921.438 kb on - strand at 3921.438 kb on - strand at 3921.450 kb on + strand at 3921.451 kb on - strand at 3921.594 kb on + strand at 3921.594 kb on + strand at 3921.678 kb on - strand at 3921.678 kb on - strand at 3921.678 kb on - strand at 3921.706 kb on - strand, within AMB_RS18050 at 3921.779 kb on + strand, within AMB_RS18050 at 3921.780 kb on - strand, within AMB_RS18050 at 3921.780 kb on - strand, within AMB_RS18050 at 3921.780 kb on - strand, within AMB_RS18050 at 3921.780 kb on - strand, within AMB_RS18050 at 3921.794 kb on + strand, within AMB_RS18050 at 3921.794 kb on + strand, within AMB_RS18050 at 3921.795 kb on - strand, within AMB_RS18050 at 3922.097 kb on + strand, within AMB_RS18050 at 3922.097 kb on + strand, within AMB_RS18050 at 3922.098 kb on - strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.109 kb on + strand, within AMB_RS18050 at 3922.110 kb on - strand, within AMB_RS18050 at 3922.110 kb on - strand, within AMB_RS18050 at 3922.110 kb on - strand, within AMB_RS18050 at 3922.110 kb on - strand, within AMB_RS18050 at 3922.115 kb on + strand, within AMB_RS18050 at 3922.115 kb on + strand, within AMB_RS18050 at 3922.116 kb on - strand, within AMB_RS18050 at 3922.238 kb on + strand, within AMB_RS18050 at 3922.238 kb on + strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.239 kb on - strand, within AMB_RS18050 at 3922.241 kb on + strand, within AMB_RS18050 at 3922.496 kb on + strand, within AMB_RS18050 at 3922.496 kb on + strand, within AMB_RS18050 at 3922.497 kb on - strand, within AMB_RS18050 at 3922.497 kb on - strand, within AMB_RS18050 at 3922.643 kb on - strand at 3922.759 kb on + strand at 3922.759 kb on + strand at 3922.759 kb on + strand at 3922.759 kb on + strand at 3922.759 kb on + strand at 3922.760 kb on - strand at 3922.760 kb on - strand at 3922.771 kb on + strand at 3922.771 kb on + strand at 3922.771 kb on + strand at 3923.223 kb on + strand, within AMB_RS18055 at 3923.223 kb on + strand, within AMB_RS18055 at 3923.223 kb on + strand, within AMB_RS18055 at 3923.223 kb on + strand, within AMB_RS18055 at 3923.223 kb on + strand, within AMB_RS18055 at 3923.224 kb on - strand, within AMB_RS18055 at 3923.232 kb on + strand, within AMB_RS18055 at 3923.517 kb on - strand, within AMB_RS18055
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 3,920,687 - +0.4 3,920,687 - +1.4 3,920,785 + AMB_RS18045 0.14 -0.1 3,920,785 + AMB_RS18045 0.14 +0.6 3,920,786 - AMB_RS18045 0.14 +0.2 3,920,786 - AMB_RS18045 0.14 -0.3 3,921,242 + AMB_RS18045 0.69 -0.9 3,921,326 + AMB_RS18045 0.79 +0.6 3,921,437 + -0.4 3,921,438 - +0.9 3,921,438 - -0.5 3,921,450 + -0.1 3,921,451 - +0.3 3,921,594 + -1.9 3,921,594 + -0.4 3,921,678 - -1.1 3,921,678 - +1.0 3,921,678 - +0.4 3,921,706 - AMB_RS18050 0.12 -0.4 3,921,779 + AMB_RS18050 0.19 +0.4 3,921,780 - AMB_RS18050 0.19 -1.2 3,921,780 - AMB_RS18050 0.19 -0.2 3,921,780 - AMB_RS18050 0.19 +1.6 3,921,780 - AMB_RS18050 0.19 -1.8 3,921,794 + AMB_RS18050 0.20 +0.8 3,921,794 + AMB_RS18050 0.20 +0.4 3,921,795 - AMB_RS18050 0.21 -0.2 3,922,097 + AMB_RS18050 0.50 -0.8 3,922,097 + AMB_RS18050 0.50 -0.3 3,922,098 - AMB_RS18050 0.50 -0.0 3,922,109 + AMB_RS18050 0.51 -0.3 3,922,109 + AMB_RS18050 0.51 +1.2 3,922,109 + AMB_RS18050 0.51 +0.4 3,922,109 + AMB_RS18050 0.51 +0.3 3,922,109 + AMB_RS18050 0.51 +0.3 3,922,109 + AMB_RS18050 0.51 -0.3 3,922,110 - AMB_RS18050 0.52 -0.8 3,922,110 - AMB_RS18050 0.52 -0.2 3,922,110 - AMB_RS18050 0.52 -0.0 3,922,110 - AMB_RS18050 0.52 -0.9 3,922,115 + AMB_RS18050 0.52 -1.3 3,922,115 + AMB_RS18050 0.52 -0.6 3,922,116 - AMB_RS18050 0.52 +0.4 3,922,238 + AMB_RS18050 0.64 -0.1 3,922,238 + AMB_RS18050 0.64 -0.8 3,922,239 - AMB_RS18050 0.64 +0.0 3,922,239 - AMB_RS18050 0.64 +0.3 3,922,239 - AMB_RS18050 0.64 -1.4 3,922,239 - AMB_RS18050 0.64 +0.0 3,922,239 - AMB_RS18050 0.64 -0.0 3,922,239 - AMB_RS18050 0.64 +0.1 3,922,241 + AMB_RS18050 0.64 -0.2 3,922,496 + AMB_RS18050 0.89 -2.9 3,922,496 + AMB_RS18050 0.89 +0.4 3,922,497 - AMB_RS18050 0.90 +0.6 3,922,497 - AMB_RS18050 0.90 -0.0 3,922,643 - -0.6 3,922,759 + -0.7 3,922,759 + -0.6 3,922,759 + +0.1 3,922,759 + -1.5 3,922,759 + -0.1 3,922,760 - -2.6 3,922,760 - -0.2 3,922,771 + -0.3 3,922,771 + +0.5 3,922,771 + -0.6 3,923,223 + AMB_RS18055 0.41 -0.5 3,923,223 + AMB_RS18055 0.41 -0.5 3,923,223 + AMB_RS18055 0.41 -0.1 3,923,223 + AMB_RS18055 0.41 -0.1 3,923,223 + AMB_RS18055 0.41 -0.4 3,923,224 - AMB_RS18055 0.41 -0.6 3,923,232 + AMB_RS18055 0.42 -0.4 3,923,517 - AMB_RS18055 0.69 -0.2
Or see this region's nucleotide sequence