Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17590 and AMB_RS17595 are separated by 77 nucleotides AMB_RS17595 and AMB_RS17600 overlap by 8 nucleotides AMB_RS17600 and AMB_RS17605 overlap by 28 nucleotides
AMB_RS17590: AMB_RS17590 - HlyD family type I secretion periplasmic adaptor subunit, at 3,820,468 to 3,822,237
_RS17590
AMB_RS17595: AMB_RS17595 - carboxymuconolactone decarboxylase family protein, at 3,822,315 to 3,822,755
_RS17595
AMB_RS17600: AMB_RS17600 - DNA-directed RNA polymerase specialized sigma subunit, at 3,822,748 to 3,823,614
_RS17600
AMB_RS17605: AMB_RS17605 - phosphoserine phosphatase SerB, at 3,823,587 to 3,824,483
_RS17605
Position (kb)
3822
3823 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3821.449 kb on + strand, within AMB_RS17590 at 3821.449 kb on + strand, within AMB_RS17590 at 3821.449 kb on + strand, within AMB_RS17590 at 3821.450 kb on - strand, within AMB_RS17590 at 3821.874 kb on - strand, within AMB_RS17590 at 3821.874 kb on - strand, within AMB_RS17590 at 3821.929 kb on + strand, within AMB_RS17590 at 3821.929 kb on + strand, within AMB_RS17590 at 3821.929 kb on + strand, within AMB_RS17590 at 3821.930 kb on - strand, within AMB_RS17590 at 3821.930 kb on - strand, within AMB_RS17590 at 3821.977 kb on + strand, within AMB_RS17590 at 3821.987 kb on - strand, within AMB_RS17590 at 3822.061 kb on + strand at 3822.061 kb on + strand at 3822.062 kb on - strand at 3822.062 kb on - strand at 3822.094 kb on + strand at 3822.094 kb on + strand at 3822.095 kb on - strand at 3822.097 kb on - strand at 3822.239 kb on - strand at 3822.239 kb on - strand at 3822.291 kb on - strand at 3822.291 kb on - strand at 3822.291 kb on - strand at 3822.291 kb on - strand at 3822.320 kb on + strand at 3822.321 kb on - strand at 3822.357 kb on + strand at 3822.357 kb on + strand at 3822.358 kb on - strand at 3822.370 kb on - strand, within AMB_RS17595 at 3822.370 kb on - strand, within AMB_RS17595 at 3822.666 kb on + strand, within AMB_RS17595 at 3822.667 kb on - strand, within AMB_RS17595 at 3822.669 kb on - strand, within AMB_RS17595 at 3822.732 kb on + strand at 3822.732 kb on + strand at 3822.818 kb on - strand at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.125 kb on - strand, within AMB_RS17600 at 3823.292 kb on - strand, within AMB_RS17600 at 3823.555 kb on + strand at 3823.556 kb on - strand at 3823.589 kb on - strand at 3823.589 kb on - strand at 3823.612 kb on + strand at 3823.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 3,821,449 + AMB_RS17590 0.55 +2.5 3,821,449 + AMB_RS17590 0.55 -0.1 3,821,449 + AMB_RS17590 0.55 -0.6 3,821,450 - AMB_RS17590 0.55 +1.0 3,821,874 - AMB_RS17590 0.79 -0.1 3,821,874 - AMB_RS17590 0.79 +0.6 3,821,929 + AMB_RS17590 0.83 +0.5 3,821,929 + AMB_RS17590 0.83 -0.6 3,821,929 + AMB_RS17590 0.83 +0.7 3,821,930 - AMB_RS17590 0.83 +0.0 3,821,930 - AMB_RS17590 0.83 -0.6 3,821,977 + AMB_RS17590 0.85 +0.8 3,821,987 - AMB_RS17590 0.86 -1.0 3,822,061 + +0.3 3,822,061 + +0.5 3,822,062 - -0.3 3,822,062 - +1.1 3,822,094 + -0.2 3,822,094 + +0.8 3,822,095 - +1.0 3,822,097 - +0.4 3,822,239 - +0.4 3,822,239 - -0.5 3,822,291 - -0.6 3,822,291 - +0.4 3,822,291 - -2.5 3,822,291 - -1.6 3,822,320 + -1.0 3,822,321 - -0.1 3,822,357 + -0.3 3,822,357 + -1.4 3,822,358 - -0.4 3,822,370 - AMB_RS17595 0.12 -1.5 3,822,370 - AMB_RS17595 0.12 +1.0 3,822,666 + AMB_RS17595 0.80 -0.6 3,822,667 - AMB_RS17595 0.80 -0.1 3,822,669 - AMB_RS17595 0.80 +0.6 3,822,732 + -1.1 3,822,732 + +0.2 3,822,818 - +0.8 3,823,099 + AMB_RS17600 0.40 -1.8 3,823,099 + AMB_RS17600 0.40 -0.5 3,823,099 + AMB_RS17600 0.40 -0.5 3,823,099 + AMB_RS17600 0.40 -1.6 3,823,099 + AMB_RS17600 0.40 -0.5 3,823,100 - AMB_RS17600 0.41 +2.7 3,823,100 - AMB_RS17600 0.41 -3.1 3,823,100 - AMB_RS17600 0.41 -0.2 3,823,124 + AMB_RS17600 0.43 +0.7 3,823,124 + AMB_RS17600 0.43 +0.0 3,823,124 + AMB_RS17600 0.43 -0.1 3,823,125 - AMB_RS17600 0.43 -0.2 3,823,292 - AMB_RS17600 0.63 +0.4 3,823,555 + +0.7 3,823,556 - -0.1 3,823,589 - -0.4 3,823,589 - -0.4 3,823,612 + +0.6 3,823,624 + -1.3
Or see this region's nucleotide sequence