Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14415 and AMB_RS14420 overlap by 1 nucleotides AMB_RS14420 and AMB_RS14425 overlap by 4 nucleotides AMB_RS14425 and AMB_RS14430 are separated by 159 nucleotides AMB_RS14430 and AMB_RS14435 are separated by 1 nucleotides
AMB_RS14415: AMB_RS14415 - indole-3-glycerol phosphate synthase TrpC, at 3,063,963 to 3,064,778
_RS14415
AMB_RS14420: AMB_RS14420 - cyclic pyranopterin monophosphate synthase MoaC, at 3,064,778 to 3,065,275
_RS14420
AMB_RS14425: AMB_RS14425 - molybdopterin molybdenumtransferase MoeA, at 3,065,272 to 3,066,474
_RS14425
AMB_RS14430: AMB_RS14430 - LexA repressor, at 3,066,634 to 3,067,341
_RS14430
AMB_RS14435: AMB_RS14435 - hypothetical protein, at 3,067,343 to 3,067,549
_RS14435
Position (kb)
3065
3066
3067 Strain fitness (log2 ratio)
-1
0
1 at 3065.259 kb on - strand at 3066.022 kb on + strand, within AMB_RS14425 at 3066.023 kb on - strand, within AMB_RS14425 at 3066.073 kb on + strand, within AMB_RS14425 at 3066.074 kb on - strand, within AMB_RS14425 at 3066.313 kb on - strand, within AMB_RS14425 at 3066.473 kb on - strand at 3066.473 kb on - strand at 3066.473 kb on - strand at 3067.076 kb on - strand, within AMB_RS14430 at 3067.076 kb on - strand, within AMB_RS14430 at 3067.267 kb on + strand, within AMB_RS14430 at 3067.336 kb on + strand at 3067.427 kb on + strand, within AMB_RS14435 at 3067.428 kb on - strand, within AMB_RS14435
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 3,065,259 - -0.4 3,066,022 + AMB_RS14425 0.62 +0.1 3,066,023 - AMB_RS14425 0.62 -0.9 3,066,073 + AMB_RS14425 0.67 -0.9 3,066,074 - AMB_RS14425 0.67 +0.4 3,066,313 - AMB_RS14425 0.87 -0.5 3,066,473 - +1.0 3,066,473 - -0.3 3,066,473 - -1.6 3,067,076 - AMB_RS14430 0.62 +0.1 3,067,076 - AMB_RS14430 0.62 -0.8 3,067,267 + AMB_RS14430 0.89 -0.2 3,067,336 + -0.4 3,067,427 + AMB_RS14435 0.41 +0.4 3,067,428 - AMB_RS14435 0.41 -1.1
Or see this region's nucleotide sequence