Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14245 and AMB_RS14250 are separated by 215 nucleotides AMB_RS14250 and AMB_RS14255 overlap by 4 nucleotides AMB_RS14255 and AMB_RS14260 are separated by 27 nucleotides
AMB_RS14245: AMB_RS14245 - DUF1849 domain-containing protein, at 3,028,655 to 3,029,461
_RS14245
AMB_RS14250: AMB_RS14250 - hypothetical protein, at 3,029,677 to 3,029,943
_RS14250
AMB_RS14255: AMB_RS14255 - hypothetical protein, at 3,029,940 to 3,030,440
_RS14255
AMB_RS14260: AMB_RS14260 - hypothetical protein, at 3,030,468 to 3,031,862
_RS14260
Position (kb)
3029
3030
3031 Strain fitness (log2 ratio)
-2
-1
0
1 at 3029.227 kb on - strand, within AMB_RS14245 at 3029.453 kb on - strand at 3029.453 kb on - strand at 3029.494 kb on - strand at 3029.495 kb on + strand at 3029.676 kb on + strand at 3029.676 kb on + strand at 3029.676 kb on + strand at 3029.677 kb on - strand at 3029.946 kb on - strand at 3030.177 kb on + strand, within AMB_RS14255 at 3030.177 kb on + strand, within AMB_RS14255 at 3030.177 kb on + strand, within AMB_RS14255 at 3030.178 kb on - strand, within AMB_RS14255 at 3030.178 kb on - strand, within AMB_RS14255 at 3030.178 kb on - strand, within AMB_RS14255 at 3030.178 kb on - strand, within AMB_RS14255 at 3030.178 kb on - strand, within AMB_RS14255 at 3030.349 kb on - strand, within AMB_RS14255 at 3030.438 kb on + strand at 3030.469 kb on + strand at 3030.469 kb on + strand at 3030.470 kb on - strand at 3030.478 kb on + strand at 3030.478 kb on + strand at 3030.479 kb on - strand at 3030.572 kb on - strand at 3030.572 kb on - strand at 3030.643 kb on + strand, within AMB_RS14260 at 3030.643 kb on + strand, within AMB_RS14260 at 3030.643 kb on + strand, within AMB_RS14260 at 3030.643 kb on + strand, within AMB_RS14260 at 3030.644 kb on - strand, within AMB_RS14260 at 3030.644 kb on - strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.646 kb on + strand, within AMB_RS14260 at 3030.647 kb on - strand, within AMB_RS14260 at 3030.647 kb on - strand, within AMB_RS14260 at 3030.647 kb on - strand, within AMB_RS14260 at 3030.647 kb on - strand, within AMB_RS14260 at 3030.923 kb on - strand, within AMB_RS14260 at 3030.923 kb on - strand, within AMB_RS14260 at 3030.935 kb on - strand, within AMB_RS14260 at 3031.120 kb on + strand, within AMB_RS14260 at 3031.120 kb on + strand, within AMB_RS14260 at 3031.120 kb on + strand, within AMB_RS14260 at 3031.120 kb on + strand, within AMB_RS14260 at 3031.121 kb on - strand, within AMB_RS14260 at 3031.121 kb on - strand, within AMB_RS14260 at 3031.121 kb on - strand, within AMB_RS14260 at 3031.121 kb on - strand, within AMB_RS14260 at 3031.121 kb on - strand, within AMB_RS14260 at 3031.127 kb on - strand, within AMB_RS14260 at 3031.127 kb on - strand, within AMB_RS14260 at 3031.177 kb on + strand, within AMB_RS14260
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 3,029,227 - AMB_RS14245 0.71 -0.5 3,029,453 - -0.6 3,029,453 - +0.1 3,029,494 - -1.4 3,029,495 + -0.9 3,029,676 + -1.0 3,029,676 + -0.6 3,029,676 + +1.0 3,029,677 - -0.8 3,029,946 - -1.8 3,030,177 + AMB_RS14255 0.47 -0.1 3,030,177 + AMB_RS14255 0.47 +0.1 3,030,177 + AMB_RS14255 0.47 +0.5 3,030,178 - AMB_RS14255 0.48 -1.6 3,030,178 - AMB_RS14255 0.48 +1.4 3,030,178 - AMB_RS14255 0.48 -0.4 3,030,178 - AMB_RS14255 0.48 -0.4 3,030,178 - AMB_RS14255 0.48 -0.4 3,030,349 - AMB_RS14255 0.82 -1.6 3,030,438 + -0.2 3,030,469 + -0.1 3,030,469 + -0.1 3,030,470 - -0.1 3,030,478 + +0.8 3,030,478 + -0.6 3,030,479 - -0.4 3,030,572 - -1.6 3,030,572 - -0.5 3,030,643 + AMB_RS14260 0.13 +0.1 3,030,643 + AMB_RS14260 0.13 +0.1 3,030,643 + AMB_RS14260 0.13 -1.6 3,030,643 + AMB_RS14260 0.13 -1.2 3,030,644 - AMB_RS14260 0.13 +0.4 3,030,644 - AMB_RS14260 0.13 -0.7 3,030,646 + AMB_RS14260 0.13 +0.1 3,030,646 + AMB_RS14260 0.13 +0.1 3,030,646 + AMB_RS14260 0.13 -0.5 3,030,646 + AMB_RS14260 0.13 -0.2 3,030,646 + AMB_RS14260 0.13 -0.3 3,030,646 + AMB_RS14260 0.13 -0.9 3,030,646 + AMB_RS14260 0.13 +0.5 3,030,647 - AMB_RS14260 0.13 +0.2 3,030,647 - AMB_RS14260 0.13 -0.6 3,030,647 - AMB_RS14260 0.13 +0.2 3,030,647 - AMB_RS14260 0.13 +0.6 3,030,923 - AMB_RS14260 0.33 -1.0 3,030,923 - AMB_RS14260 0.33 -0.1 3,030,935 - AMB_RS14260 0.33 +0.3 3,031,120 + AMB_RS14260 0.47 +0.3 3,031,120 + AMB_RS14260 0.47 -0.3 3,031,120 + AMB_RS14260 0.47 -0.4 3,031,120 + AMB_RS14260 0.47 -0.3 3,031,121 - AMB_RS14260 0.47 +0.4 3,031,121 - AMB_RS14260 0.47 -1.2 3,031,121 - AMB_RS14260 0.47 -0.6 3,031,121 - AMB_RS14260 0.47 +0.5 3,031,121 - AMB_RS14260 0.47 -0.3 3,031,127 - AMB_RS14260 0.47 -0.3 3,031,127 - AMB_RS14260 0.47 -0.5 3,031,177 + AMB_RS14260 0.51 +0.2
Or see this region's nucleotide sequence