Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12275 and AMB_RS12280 overlap by 1 nucleotides AMB_RS12280 and AMB_RS12285 are separated by 442 nucleotides AMB_RS12285 and AMB_RS12290 overlap by 11 nucleotides AMB_RS12290 and AMB_RS12295 overlap by 4 nucleotides
AMB_RS12270: AMB_RS12270 - hypothetical protein, at 2,619,971 to 2,620,276
_RS12270
AMB_RS12275: AMB_RS12275 - hypothetical protein, at 2,620,260 to 2,620,463
_RS12275
AMB_RS12280: AMB_RS12280 - DUF736 domain-containing protein, at 2,620,463 to 2,620,828
_RS12280
AMB_RS12285: AMB_RS12285 - Na+/H+ antiporter NhaA, at 2,621,271 to 2,622,422
_RS12285
AMB_RS12290: AMB_RS12290 - MBL fold metallo-hydrolase, at 2,622,412 to 2,623,185
_RS12290
AMB_RS12295: AMB_RS12295 - TatD family deoxyribonuclease, at 2,623,182 to 2,623,961
_RS12295
Position (kb)
2621
2622
2623 Strain fitness (log2 ratio)
-2
-1
0
1 at 2621.201 kb on + strand at 2621.201 kb on + strand at 2621.202 kb on - strand at 2621.267 kb on + strand at 2621.268 kb on - strand at 2621.268 kb on - strand at 2621.272 kb on + strand at 2621.272 kb on + strand at 2622.716 kb on + strand, within AMB_RS12290 at 2622.716 kb on + strand, within AMB_RS12290 at 2622.803 kb on + strand, within AMB_RS12290 at 2622.803 kb on + strand, within AMB_RS12290 at 2622.971 kb on + strand, within AMB_RS12290 at 2622.971 kb on + strand, within AMB_RS12290 at 2623.298 kb on - strand, within AMB_RS12295 at 2623.298 kb on - strand, within AMB_RS12295
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 2,621,201 + -0.4 2,621,201 + -0.5 2,621,202 - -1.9 2,621,267 + -1.0 2,621,268 - -0.6 2,621,268 - -1.6 2,621,272 + -1.9 2,621,272 + -0.0 2,622,716 + AMB_RS12290 0.39 +0.7 2,622,716 + AMB_RS12290 0.39 +0.0 2,622,803 + AMB_RS12290 0.51 -0.5 2,622,803 + AMB_RS12290 0.51 -1.2 2,622,971 + AMB_RS12290 0.72 -1.9 2,622,971 + AMB_RS12290 0.72 -1.1 2,623,298 - AMB_RS12295 0.15 -0.6 2,623,298 - AMB_RS12295 0.15 -0.8
Or see this region's nucleotide sequence