Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10680 and AMB_RS10685 are separated by 273 nucleotides AMB_RS10685 and AMB_RS10690 are separated by 256 nucleotides AMB_RS10690 and AMB_RS10695 are separated by 205 nucleotides
AMB_RS10680: AMB_RS10680 - monothiol glutaredoxin, Grx4 family, at 2,291,809 to 2,292,138
_RS10680
AMB_RS10685: AMB_RS10685 - hypothetical protein, at 2,292,412 to 2,292,855
_RS10685
AMB_RS10690: AMB_RS10690 - hypothetical protein, at 2,293,112 to 2,293,384
_RS10690
AMB_RS10695: AMB_RS10695 - hypothetical protein, at 2,293,590 to 2,294,468
_RS10695
Position (kb)
2293
2294 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2292.497 kb on - strand, within AMB_RS10685 at 2292.689 kb on + strand, within AMB_RS10685 at 2292.689 kb on + strand, within AMB_RS10685 at 2292.690 kb on - strand, within AMB_RS10685 at 2292.690 kb on - strand, within AMB_RS10685 at 2292.892 kb on + strand at 2292.893 kb on - strand at 2292.970 kb on - strand at 2292.970 kb on - strand at 2292.976 kb on + strand at 2292.976 kb on + strand at 2292.977 kb on - strand at 2292.977 kb on - strand at 2292.979 kb on + strand at 2293.055 kb on + strand at 2293.055 kb on + strand at 2293.055 kb on + strand at 2293.055 kb on + strand at 2293.056 kb on - strand at 2293.056 kb on - strand at 2293.076 kb on + strand at 2293.076 kb on + strand at 2293.076 kb on + strand at 2293.076 kb on + strand at 2293.077 kb on - strand at 2293.077 kb on - strand at 2293.077 kb on - strand at 2293.077 kb on - strand at 2293.077 kb on - strand at 2293.080 kb on + strand at 2293.080 kb on + strand at 2293.080 kb on + strand at 2293.080 kb on + strand at 2293.081 kb on - strand at 2293.081 kb on - strand at 2293.081 kb on - strand at 2293.081 kb on - strand at 2293.160 kb on + strand, within AMB_RS10690 at 2293.204 kb on + strand, within AMB_RS10690 at 2293.204 kb on + strand, within AMB_RS10690 at 2293.205 kb on - strand, within AMB_RS10690 at 2293.284 kb on + strand, within AMB_RS10690 at 2293.284 kb on + strand, within AMB_RS10690 at 2293.284 kb on + strand, within AMB_RS10690 at 2293.284 kb on + strand, within AMB_RS10690 at 2293.285 kb on - strand, within AMB_RS10690 at 2293.285 kb on - strand, within AMB_RS10690 at 2293.362 kb on + strand at 2293.362 kb on + strand at 2293.362 kb on + strand at 2293.362 kb on + strand at 2293.418 kb on + strand at 2293.644 kb on - strand at 2293.704 kb on + strand, within AMB_RS10695 at 2293.704 kb on + strand, within AMB_RS10695 at 2293.704 kb on + strand, within AMB_RS10695 at 2293.704 kb on + strand, within AMB_RS10695 at 2293.704 kb on + strand, within AMB_RS10695 at 2293.705 kb on - strand, within AMB_RS10695 at 2293.705 kb on - strand, within AMB_RS10695 at 2293.705 kb on - strand, within AMB_RS10695 at 2293.705 kb on - strand, within AMB_RS10695 at 2293.741 kb on + strand, within AMB_RS10695 at 2293.742 kb on - strand, within AMB_RS10695 at 2293.742 kb on - strand, within AMB_RS10695 at 2293.742 kb on - strand, within AMB_RS10695 at 2293.742 kb on - strand, within AMB_RS10695 at 2293.923 kb on + strand, within AMB_RS10695 at 2293.924 kb on - strand, within AMB_RS10695 at 2293.924 kb on - strand, within AMB_RS10695 at 2294.044 kb on + strand, within AMB_RS10695 at 2294.045 kb on - strand, within AMB_RS10695 at 2294.045 kb on - strand, within AMB_RS10695 at 2294.124 kb on - strand, within AMB_RS10695 at 2294.156 kb on - strand, within AMB_RS10695 at 2294.202 kb on - strand, within AMB_RS10695 at 2294.202 kb on - strand, within AMB_RS10695 at 2294.224 kb on - strand, within AMB_RS10695 at 2294.292 kb on + strand, within AMB_RS10695 at 2294.292 kb on + strand, within AMB_RS10695 at 2294.293 kb on - strand, within AMB_RS10695 at 2294.293 kb on - strand, within AMB_RS10695 at 2294.361 kb on - strand, within AMB_RS10695 at 2294.370 kb on + strand, within AMB_RS10695 at 2294.370 kb on + strand, within AMB_RS10695
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 2,292,497 - AMB_RS10685 0.19 +0.4 2,292,689 + AMB_RS10685 0.62 -0.3 2,292,689 + AMB_RS10685 0.62 -0.1 2,292,690 - AMB_RS10685 0.63 -0.6 2,292,690 - AMB_RS10685 0.63 -1.7 2,292,892 + -0.4 2,292,893 - -0.2 2,292,970 - +0.8 2,292,970 - -0.4 2,292,976 + +0.1 2,292,976 + -1.6 2,292,977 - -0.5 2,292,977 - -1.4 2,292,979 + -0.1 2,293,055 + -1.1 2,293,055 + -0.6 2,293,055 + -0.5 2,293,055 + +0.7 2,293,056 - -0.5 2,293,056 - +0.2 2,293,076 + -0.6 2,293,076 + -0.3 2,293,076 + +0.3 2,293,076 + -0.3 2,293,077 - -0.2 2,293,077 - -0.3 2,293,077 - +0.4 2,293,077 - +0.2 2,293,077 - -0.9 2,293,080 + +0.4 2,293,080 + -0.2 2,293,080 + -0.2 2,293,080 + -1.1 2,293,081 - +0.1 2,293,081 - +0.4 2,293,081 - -1.3 2,293,081 - +1.4 2,293,160 + AMB_RS10690 0.18 -0.4 2,293,204 + AMB_RS10690 0.34 -1.8 2,293,204 + AMB_RS10690 0.34 -0.4 2,293,205 - AMB_RS10690 0.34 -0.7 2,293,284 + AMB_RS10690 0.63 -0.5 2,293,284 + AMB_RS10690 0.63 +0.7 2,293,284 + AMB_RS10690 0.63 -0.9 2,293,284 + AMB_RS10690 0.63 -0.6 2,293,285 - AMB_RS10690 0.63 -0.2 2,293,285 - AMB_RS10690 0.63 +0.4 2,293,362 + -0.5 2,293,362 + -0.3 2,293,362 + -1.2 2,293,362 + -0.6 2,293,418 + -0.4 2,293,644 - -1.4 2,293,704 + AMB_RS10695 0.13 -0.6 2,293,704 + AMB_RS10695 0.13 -0.0 2,293,704 + AMB_RS10695 0.13 -3.2 2,293,704 + AMB_RS10695 0.13 -0.3 2,293,704 + AMB_RS10695 0.13 -0.2 2,293,705 - AMB_RS10695 0.13 -0.2 2,293,705 - AMB_RS10695 0.13 +1.0 2,293,705 - AMB_RS10695 0.13 -0.4 2,293,705 - AMB_RS10695 0.13 -0.7 2,293,741 + AMB_RS10695 0.17 +0.9 2,293,742 - AMB_RS10695 0.17 -0.3 2,293,742 - AMB_RS10695 0.17 -0.7 2,293,742 - AMB_RS10695 0.17 +0.4 2,293,742 - AMB_RS10695 0.17 +0.8 2,293,923 + AMB_RS10695 0.38 +2.2 2,293,924 - AMB_RS10695 0.38 -1.1 2,293,924 - AMB_RS10695 0.38 -0.1 2,294,044 + AMB_RS10695 0.52 +0.4 2,294,045 - AMB_RS10695 0.52 +0.2 2,294,045 - AMB_RS10695 0.52 +0.3 2,294,124 - AMB_RS10695 0.61 -0.8 2,294,156 - AMB_RS10695 0.64 -1.2 2,294,202 - AMB_RS10695 0.70 -0.1 2,294,202 - AMB_RS10695 0.70 +0.1 2,294,224 - AMB_RS10695 0.72 +0.1 2,294,292 + AMB_RS10695 0.80 -0.2 2,294,292 + AMB_RS10695 0.80 -0.2 2,294,293 - AMB_RS10695 0.80 -0.9 2,294,293 - AMB_RS10695 0.80 -0.1 2,294,361 - AMB_RS10695 0.88 -1.6 2,294,370 + AMB_RS10695 0.89 -0.6 2,294,370 + AMB_RS10695 0.89 +0.9
Or see this region's nucleotide sequence