Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10280 and AMB_RS10285 are separated by 138 nucleotides AMB_RS10285 and AMB_RS10290 are separated by 568 nucleotides AMB_RS10290 and AMB_RS10295 overlap by 4 nucleotides AMB_RS10295 and AMB_RS10300 are separated by 11 nucleotides
AMB_RS10280: AMB_RS10280 - hypothetical protein, at 2,214,256 to 2,214,564
_RS10280
AMB_RS10285: AMB_RS10285 - hypothetical protein, at 2,214,703 to 2,214,957
_RS10285
AMB_RS10290: AMB_RS10290 - DUF1819 domain-containing protein, at 2,215,526 to 2,216,125
_RS10290
AMB_RS10295: AMB_RS10295 - DUF1788 domain-containing protein, at 2,216,122 to 2,216,694
_RS10295
AMB_RS10300: AMB_RS10300 - hypothetical protein, at 2,216,706 to 2,219,075
_RS10300
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2214.696 kb on - strand at 2214.836 kb on - strand, within AMB_RS10285 at 2214.861 kb on + strand, within AMB_RS10285 at 2215.135 kb on - strand at 2215.166 kb on + strand at 2215.450 kb on + strand at 2215.451 kb on - strand at 2215.541 kb on + strand at 2215.541 kb on + strand at 2215.542 kb on - strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.553 kb on - strand at 2215.753 kb on - strand, within AMB_RS10290 at 2215.753 kb on - strand, within AMB_RS10290 at 2215.901 kb on + strand, within AMB_RS10290 at 2215.902 kb on - strand, within AMB_RS10290 at 2215.902 kb on - strand, within AMB_RS10290 at 2215.982 kb on + strand, within AMB_RS10290 at 2216.006 kb on + strand, within AMB_RS10290 at 2216.007 kb on - strand, within AMB_RS10290 at 2216.007 kb on - strand, within AMB_RS10290 at 2216.027 kb on + strand, within AMB_RS10290 at 2216.069 kb on + strand at 2216.069 kb on + strand at 2216.069 kb on + strand at 2216.070 kb on - strand at 2216.070 kb on - strand at 2216.070 kb on - strand at 2216.070 kb on - strand at 2216.084 kb on - strand at 2216.093 kb on + strand at 2216.093 kb on + strand at 2216.097 kb on + strand at 2216.097 kb on + strand at 2216.098 kb on - strand at 2216.098 kb on - strand at 2216.122 kb on - strand at 2216.145 kb on - strand at 2216.250 kb on + strand, within AMB_RS10295 at 2216.251 kb on - strand, within AMB_RS10295 at 2216.251 kb on - strand, within AMB_RS10295 at 2216.252 kb on - strand, within AMB_RS10295 at 2216.337 kb on + strand, within AMB_RS10295 at 2216.338 kb on - strand, within AMB_RS10295 at 2216.510 kb on + strand, within AMB_RS10295 at 2216.550 kb on + strand, within AMB_RS10295 at 2216.550 kb on + strand, within AMB_RS10295 at 2216.551 kb on - strand, within AMB_RS10295 at 2216.705 kb on + strand at 2216.705 kb on + strand at 2216.705 kb on + strand at 2216.706 kb on - strand at 2216.706 kb on - strand at 2216.706 kb on - strand at 2216.811 kb on + strand at 2216.812 kb on - strand at 2216.973 kb on + strand, within AMB_RS10300 at 2216.988 kb on + strand, within AMB_RS10300
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 2,214,696 - +3.0 2,214,836 - AMB_RS10285 0.52 -0.2 2,214,861 + AMB_RS10285 0.62 +0.3 2,215,135 - -1.0 2,215,166 + +0.1 2,215,450 + -0.3 2,215,451 - +0.6 2,215,541 + -0.5 2,215,541 + +0.1 2,215,542 - -0.4 2,215,552 + -0.0 2,215,552 + -0.7 2,215,552 + +0.0 2,215,552 + +0.0 2,215,552 + -1.4 2,215,553 - -1.0 2,215,753 - AMB_RS10290 0.38 -0.0 2,215,753 - AMB_RS10290 0.38 +0.7 2,215,901 + AMB_RS10290 0.62 -0.4 2,215,902 - AMB_RS10290 0.63 +0.3 2,215,902 - AMB_RS10290 0.63 +0.0 2,215,982 + AMB_RS10290 0.76 -1.2 2,216,006 + AMB_RS10290 0.80 -0.5 2,216,007 - AMB_RS10290 0.80 +1.5 2,216,007 - AMB_RS10290 0.80 -0.6 2,216,027 + AMB_RS10290 0.83 +0.5 2,216,069 + -0.4 2,216,069 + +0.0 2,216,069 + -0.6 2,216,070 - +1.3 2,216,070 - +0.2 2,216,070 - -0.2 2,216,070 - -0.4 2,216,084 - +0.2 2,216,093 + -0.8 2,216,093 + -0.3 2,216,097 + -1.0 2,216,097 + +0.4 2,216,098 - -0.9 2,216,098 - -0.6 2,216,122 - -1.6 2,216,145 - -0.4 2,216,250 + AMB_RS10295 0.22 +0.4 2,216,251 - AMB_RS10295 0.23 -0.3 2,216,251 - AMB_RS10295 0.23 -0.3 2,216,252 - AMB_RS10295 0.23 -0.1 2,216,337 + AMB_RS10295 0.38 -0.0 2,216,338 - AMB_RS10295 0.38 +0.2 2,216,510 + AMB_RS10295 0.68 +1.2 2,216,550 + AMB_RS10295 0.75 -0.1 2,216,550 + AMB_RS10295 0.75 +0.4 2,216,551 - AMB_RS10295 0.75 -0.3 2,216,705 + -0.8 2,216,705 + -0.4 2,216,705 + -1.0 2,216,706 - +1.6 2,216,706 - -0.8 2,216,706 - +0.4 2,216,811 + -1.6 2,216,812 - -1.1 2,216,973 + AMB_RS10300 0.11 +0.0 2,216,988 + AMB_RS10300 0.12 +0.6
Or see this region's nucleotide sequence