Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10270 and AMB_RS10275 are separated by 181 nucleotides AMB_RS10275 and AMB_RS10280 are separated by 19 nucleotides AMB_RS10280 and AMB_RS10285 are separated by 138 nucleotides AMB_RS10285 and AMB_RS10290 are separated by 568 nucleotides
AMB_RS10270: AMB_RS10270 - hypothetical protein, at 2,213,123 to 2,213,704
_RS10270
AMB_RS10275: AMB_RS10275 - hypothetical protein, at 2,213,886 to 2,214,236
_RS10275
AMB_RS10280: AMB_RS10280 - hypothetical protein, at 2,214,256 to 2,214,564
_RS10280
AMB_RS10285: AMB_RS10285 - hypothetical protein, at 2,214,703 to 2,214,957
_RS10285
AMB_RS10290: AMB_RS10290 - DUF1819 domain-containing protein, at 2,215,526 to 2,216,125
_RS10290
Position (kb)
2214
2215 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2213.839 kb on + strand at 2213.849 kb on + strand at 2213.849 kb on + strand at 2213.849 kb on + strand at 2213.849 kb on + strand at 2213.849 kb on + strand at 2213.849 kb on + strand at 2213.850 kb on - strand at 2213.850 kb on - strand at 2213.850 kb on - strand at 2213.850 kb on - strand at 2213.850 kb on - strand at 2213.850 kb on - strand at 2213.908 kb on + strand at 2213.909 kb on - strand at 2213.909 kb on - strand at 2213.909 kb on - strand at 2213.969 kb on + strand, within AMB_RS10275 at 2214.029 kb on + strand, within AMB_RS10275 at 2214.029 kb on + strand, within AMB_RS10275 at 2214.029 kb on + strand, within AMB_RS10275 at 2214.030 kb on - strand, within AMB_RS10275 at 2214.030 kb on - strand, within AMB_RS10275 at 2214.207 kb on + strand at 2214.265 kb on + strand at 2214.265 kb on + strand at 2214.266 kb on - strand at 2214.384 kb on - strand, within AMB_RS10280 at 2214.403 kb on + strand, within AMB_RS10280 at 2214.403 kb on + strand, within AMB_RS10280 at 2214.403 kb on + strand, within AMB_RS10280 at 2214.403 kb on + strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.404 kb on - strand, within AMB_RS10280 at 2214.696 kb on - strand at 2214.836 kb on - strand, within AMB_RS10285 at 2214.861 kb on + strand, within AMB_RS10285 at 2215.135 kb on - strand at 2215.166 kb on + strand at 2215.450 kb on + strand at 2215.451 kb on - strand at 2215.541 kb on + strand at 2215.541 kb on + strand at 2215.542 kb on - strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.552 kb on + strand at 2215.553 kb on - strand at 2215.753 kb on - strand, within AMB_RS10290 at 2215.753 kb on - strand, within AMB_RS10290 at 2215.901 kb on + strand, within AMB_RS10290 at 2215.902 kb on - strand, within AMB_RS10290 at 2215.902 kb on - strand, within AMB_RS10290
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 2,213,839 + -2.4 2,213,849 + +0.2 2,213,849 + -0.2 2,213,849 + -0.1 2,213,849 + -1.1 2,213,849 + -2.8 2,213,849 + -0.6 2,213,850 - -3.1 2,213,850 - -0.9 2,213,850 - +0.6 2,213,850 - +0.7 2,213,850 - -0.3 2,213,850 - -0.9 2,213,908 + -0.2 2,213,909 - -0.3 2,213,909 - -2.2 2,213,909 - -1.9 2,213,969 + AMB_RS10275 0.24 -2.2 2,214,029 + AMB_RS10275 0.41 +0.2 2,214,029 + AMB_RS10275 0.41 +0.1 2,214,029 + AMB_RS10275 0.41 -0.4 2,214,030 - AMB_RS10275 0.41 +0.0 2,214,030 - AMB_RS10275 0.41 -0.6 2,214,207 + -0.0 2,214,265 + -2.5 2,214,265 + -0.1 2,214,266 - -0.6 2,214,384 - AMB_RS10280 0.41 -0.3 2,214,403 + AMB_RS10280 0.48 -1.9 2,214,403 + AMB_RS10280 0.48 -1.2 2,214,403 + AMB_RS10280 0.48 -1.9 2,214,403 + AMB_RS10280 0.48 -0.2 2,214,404 - AMB_RS10280 0.48 +0.2 2,214,404 - AMB_RS10280 0.48 +2.2 2,214,404 - AMB_RS10280 0.48 +0.3 2,214,404 - AMB_RS10280 0.48 +0.1 2,214,404 - AMB_RS10280 0.48 -0.7 2,214,404 - AMB_RS10280 0.48 -1.2 2,214,404 - AMB_RS10280 0.48 -0.6 2,214,696 - +3.0 2,214,836 - AMB_RS10285 0.52 -0.2 2,214,861 + AMB_RS10285 0.62 +0.3 2,215,135 - -1.0 2,215,166 + +0.1 2,215,450 + -0.3 2,215,451 - +0.6 2,215,541 + -0.5 2,215,541 + +0.1 2,215,542 - -0.4 2,215,552 + -0.0 2,215,552 + -0.7 2,215,552 + +0.0 2,215,552 + +0.0 2,215,552 + -1.4 2,215,553 - -1.0 2,215,753 - AMB_RS10290 0.38 -0.0 2,215,753 - AMB_RS10290 0.38 +0.7 2,215,901 + AMB_RS10290 0.62 -0.4 2,215,902 - AMB_RS10290 0.63 +0.3 2,215,902 - AMB_RS10290 0.63 +0.0
Or see this region's nucleotide sequence