Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09315 and AMB_RS09320 overlap by 4 nucleotides AMB_RS09320 and AMB_RS09325 are separated by 20 nucleotides
AMB_RS09315: AMB_RS09315 - PAS domain S-box protein, at 1,978,629 to 1,983,479
_RS09315
AMB_RS09320: AMB_RS09320 - glycoside hydrolase family 3 protein, at 1,983,476 to 1,984,657
_RS09320
AMB_RS09325: AMB_RS09325 - cell envelope integrity protein CreD, at 1,984,678 to 1,986,042
_RS09325
Position (kb)
1983
1984
1985 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1982.708 kb on - strand, within AMB_RS09315 at 1982.779 kb on + strand, within AMB_RS09315 at 1982.779 kb on + strand, within AMB_RS09315 at 1982.779 kb on + strand, within AMB_RS09315 at 1982.779 kb on + strand, within AMB_RS09315 at 1982.779 kb on + strand, within AMB_RS09315 at 1982.780 kb on - strand, within AMB_RS09315 at 1982.858 kb on - strand, within AMB_RS09315 at 1983.222 kb on + strand at 1983.222 kb on + strand at 1983.247 kb on + strand at 1983.416 kb on + strand at 1983.417 kb on - strand at 1983.427 kb on + strand at 1983.427 kb on + strand at 1983.427 kb on + strand at 1983.427 kb on + strand at 1983.427 kb on + strand at 1983.427 kb on + strand at 1983.428 kb on - strand at 1983.428 kb on - strand at 1983.437 kb on + strand at 1983.437 kb on + strand at 1983.438 kb on - strand at 1983.438 kb on - strand at 1983.438 kb on - strand at 1983.511 kb on - strand at 1983.512 kb on + strand at 1983.512 kb on + strand at 1983.512 kb on + strand at 1983.512 kb on + strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.513 kb on - strand at 1983.597 kb on + strand, within AMB_RS09320 at 1983.597 kb on + strand, within AMB_RS09320 at 1983.597 kb on + strand, within AMB_RS09320 at 1983.598 kb on - strand, within AMB_RS09320 at 1983.919 kb on - strand, within AMB_RS09320 at 1983.919 kb on - strand, within AMB_RS09320 at 1984.089 kb on + strand, within AMB_RS09320 at 1984.090 kb on - strand, within AMB_RS09320 at 1984.600 kb on - strand at 1984.793 kb on + strand at 1984.793 kb on + strand at 1984.794 kb on - strand at 1984.794 kb on - strand at 1984.794 kb on - strand at 1984.794 kb on - strand at 1984.794 kb on - strand at 1984.794 kb on - strand at 1984.817 kb on + strand, within AMB_RS09325 at 1984.817 kb on + strand, within AMB_RS09325 at 1984.818 kb on - strand, within AMB_RS09325 at 1984.818 kb on - strand, within AMB_RS09325 at 1984.818 kb on - strand, within AMB_RS09325 at 1984.818 kb on - strand, within AMB_RS09325 at 1984.893 kb on + strand, within AMB_RS09325 at 1984.893 kb on + strand, within AMB_RS09325 at 1984.895 kb on + strand, within AMB_RS09325 at 1984.895 kb on + strand, within AMB_RS09325 at 1984.896 kb on - strand, within AMB_RS09325 at 1984.896 kb on - strand, within AMB_RS09325 at 1984.896 kb on - strand, within AMB_RS09325 at 1984.949 kb on + strand, within AMB_RS09325 at 1985.066 kb on + strand, within AMB_RS09325 at 1985.066 kb on + strand, within AMB_RS09325 at 1985.067 kb on - strand, within AMB_RS09325 at 1985.067 kb on - strand, within AMB_RS09325 at 1985.067 kb on - strand, within AMB_RS09325 at 1985.102 kb on + strand, within AMB_RS09325 at 1985.102 kb on + strand, within AMB_RS09325 at 1985.229 kb on - strand, within AMB_RS09325
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 1,982,708 - AMB_RS09315 0.84 -0.6 1,982,779 + AMB_RS09315 0.86 -0.4 1,982,779 + AMB_RS09315 0.86 +0.4 1,982,779 + AMB_RS09315 0.86 -0.3 1,982,779 + AMB_RS09315 0.86 -0.2 1,982,779 + AMB_RS09315 0.86 +0.6 1,982,780 - AMB_RS09315 0.86 +1.0 1,982,858 - AMB_RS09315 0.87 -0.5 1,983,222 + +0.6 1,983,222 + -0.3 1,983,247 + +0.9 1,983,416 + +0.1 1,983,417 - +0.4 1,983,427 + -2.4 1,983,427 + -0.4 1,983,427 + +1.0 1,983,427 + -1.0 1,983,427 + -0.4 1,983,427 + +0.9 1,983,428 - -0.0 1,983,428 - -0.5 1,983,437 + +1.4 1,983,437 + +0.5 1,983,438 - +1.1 1,983,438 - -0.2 1,983,438 - +0.4 1,983,511 - -0.1 1,983,512 + -0.1 1,983,512 + -0.1 1,983,512 + -0.4 1,983,512 + +0.4 1,983,513 - -0.4 1,983,513 - -0.4 1,983,513 - -0.1 1,983,513 - -0.5 1,983,513 - -0.0 1,983,513 - +0.3 1,983,513 - +0.4 1,983,597 + AMB_RS09320 0.10 -0.3 1,983,597 + AMB_RS09320 0.10 -0.6 1,983,597 + AMB_RS09320 0.10 -2.1 1,983,598 - AMB_RS09320 0.10 -0.9 1,983,919 - AMB_RS09320 0.37 -2.2 1,983,919 - AMB_RS09320 0.37 +0.4 1,984,089 + AMB_RS09320 0.52 -0.0 1,984,090 - AMB_RS09320 0.52 -0.6 1,984,600 - -0.8 1,984,793 + -0.5 1,984,793 + -1.8 1,984,794 - -1.0 1,984,794 - -1.1 1,984,794 - -0.3 1,984,794 - -0.5 1,984,794 - -0.1 1,984,794 - -0.4 1,984,817 + AMB_RS09325 0.10 +0.3 1,984,817 + AMB_RS09325 0.10 -0.8 1,984,818 - AMB_RS09325 0.10 -2.8 1,984,818 - AMB_RS09325 0.10 -0.8 1,984,818 - AMB_RS09325 0.10 -0.6 1,984,818 - AMB_RS09325 0.10 -0.6 1,984,893 + AMB_RS09325 0.16 -0.4 1,984,893 + AMB_RS09325 0.16 -1.8 1,984,895 + AMB_RS09325 0.16 -0.6 1,984,895 + AMB_RS09325 0.16 +0.4 1,984,896 - AMB_RS09325 0.16 -1.1 1,984,896 - AMB_RS09325 0.16 +0.6 1,984,896 - AMB_RS09325 0.16 -0.3 1,984,949 + AMB_RS09325 0.20 +1.1 1,985,066 + AMB_RS09325 0.28 +1.6 1,985,066 + AMB_RS09325 0.28 +0.9 1,985,067 - AMB_RS09325 0.28 -0.9 1,985,067 - AMB_RS09325 0.28 +0.8 1,985,067 - AMB_RS09325 0.28 -1.0 1,985,102 + AMB_RS09325 0.31 +0.1 1,985,102 + AMB_RS09325 0.31 +0.9 1,985,229 - AMB_RS09325 0.40 -0.2
Or see this region's nucleotide sequence