Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08180 and AMB_RS08185 are separated by 188 nucleotides AMB_RS08185 and AMB_RS08190 are separated by 12 nucleotides AMB_RS08190 and AMB_RS08195 are separated by 71 nucleotides
AMB_RS08180: AMB_RS08180 - peptide chain release factor N(5)-glutamine methyltransferase, at 1,748,838 to 1,749,689
_RS08180
AMB_RS08185: AMB_RS08185 - DUF4167 domain-containing protein, at 1,749,878 to 1,750,357
_RS08185
AMB_RS08190: AMB_RS08190 - hypothetical protein, at 1,750,370 to 1,750,747
_RS08190
AMB_RS08195: AMB_RS08195 - glycosyltransferase, at 1,750,819 to 1,752,393
_RS08195
Position (kb)
1750
1751 Strain fitness (log2 ratio)
-1
0
1
2 at 1749.699 kb on + strand at 1749.716 kb on + strand at 1749.861 kb on + strand at 1749.861 kb on + strand at 1750.408 kb on + strand, within AMB_RS08190 at 1750.408 kb on + strand, within AMB_RS08190 at 1750.488 kb on - strand, within AMB_RS08190 at 1750.772 kb on + strand at 1750.772 kb on + strand at 1751.113 kb on - strand, within AMB_RS08195 at 1751.306 kb on + strand, within AMB_RS08195 at 1751.306 kb on + strand, within AMB_RS08195 at 1751.306 kb on + strand, within AMB_RS08195 at 1751.306 kb on + strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.307 kb on - strand, within AMB_RS08195 at 1751.384 kb on + strand, within AMB_RS08195 at 1751.385 kb on - strand, within AMB_RS08195 at 1751.385 kb on - strand, within AMB_RS08195 at 1751.690 kb on + strand, within AMB_RS08195 at 1751.690 kb on + strand, within AMB_RS08195 at 1751.691 kb on - strand, within AMB_RS08195 at 1751.691 kb on - strand, within AMB_RS08195 at 1751.691 kb on - strand, within AMB_RS08195 at 1751.726 kb on + strand, within AMB_RS08195
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 1,749,699 + -0.5 1,749,716 + +2.3 1,749,861 + +0.2 1,749,861 + -0.0 1,750,408 + AMB_RS08190 0.10 -0.2 1,750,408 + AMB_RS08190 0.10 +0.4 1,750,488 - AMB_RS08190 0.31 -1.6 1,750,772 + -0.2 1,750,772 + -0.2 1,751,113 - AMB_RS08195 0.19 -0.1 1,751,306 + AMB_RS08195 0.31 -0.1 1,751,306 + AMB_RS08195 0.31 -0.3 1,751,306 + AMB_RS08195 0.31 -0.2 1,751,306 + AMB_RS08195 0.31 +0.1 1,751,307 - AMB_RS08195 0.31 -0.3 1,751,307 - AMB_RS08195 0.31 -0.9 1,751,307 - AMB_RS08195 0.31 +0.1 1,751,307 - AMB_RS08195 0.31 -0.0 1,751,307 - AMB_RS08195 0.31 -0.3 1,751,307 - AMB_RS08195 0.31 -1.5 1,751,307 - AMB_RS08195 0.31 -0.2 1,751,384 + AMB_RS08195 0.36 -0.1 1,751,385 - AMB_RS08195 0.36 +1.2 1,751,385 - AMB_RS08195 0.36 -0.3 1,751,690 + AMB_RS08195 0.55 +0.6 1,751,690 + AMB_RS08195 0.55 -0.6 1,751,691 - AMB_RS08195 0.55 -0.4 1,751,691 - AMB_RS08195 0.55 -0.9 1,751,691 - AMB_RS08195 0.55 -0.1 1,751,726 + AMB_RS08195 0.58 +0.7
Or see this region's nucleotide sequence