Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05160 and AMB_RS05165 overlap by 4 nucleotides AMB_RS05165 and AMB_RS05170 are separated by 59 nucleotides AMB_RS05170 and AMB_RS05175 are separated by 138 nucleotides AMB_RS05175 and AMB_RS05180 are separated by 105 nucleotides
AMB_RS05160: AMB_RS05160 - DNA-binding protein, at 1,068,179 to 1,068,433
_RS05160
AMB_RS05165: AMB_RS05165 - cation transporter, at 1,068,430 to 1,069,320
_RS05165
AMB_RS05170: AMB_RS05170 - hypothetical protein, at 1,069,380 to 1,069,628
_RS05170
AMB_RS05175: AMB_RS05175 - transcriptional repressor, at 1,069,767 to 1,070,192
_RS05175
AMB_RS05180: AMB_RS05180 - MerR family DNA-binding transcriptional regulator, at 1,070,298 to 1,070,723
_RS05180
Position (kb)
1069
1070 Strain fitness (log2 ratio)
-2
-1
0
1 at 1069.361 kb on + strand at 1069.361 kb on + strand at 1069.362 kb on - strand at 1069.362 kb on - strand at 1069.414 kb on + strand, within AMB_RS05170 at 1069.414 kb on + strand, within AMB_RS05170 at 1069.415 kb on - strand, within AMB_RS05170 at 1069.669 kb on - strand at 1069.704 kb on + strand at 1069.834 kb on + strand, within AMB_RS05175 at 1069.840 kb on + strand, within AMB_RS05175 at 1070.026 kb on + strand, within AMB_RS05175 at 1070.046 kb on - strand, within AMB_RS05175 at 1070.236 kb on - strand at 1070.301 kb on + strand at 1070.301 kb on + strand at 1070.301 kb on + strand at 1070.301 kb on + strand at 1070.301 kb on + strand at 1070.301 kb on + strand at 1070.302 kb on - strand at 1070.302 kb on - strand at 1070.302 kb on - strand at 1070.303 kb on + strand at 1070.303 kb on + strand at 1070.303 kb on + strand at 1070.303 kb on + strand at 1070.303 kb on + strand at 1070.303 kb on + strand at 1070.304 kb on - strand at 1070.304 kb on - strand at 1070.304 kb on - strand at 1070.304 kb on - strand at 1070.304 kb on - strand at 1070.593 kb on + strand, within AMB_RS05180 at 1070.593 kb on + strand, within AMB_RS05180 at 1070.593 kb on + strand, within AMB_RS05180
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 1,069,361 + +0.9 1,069,361 + -0.4 1,069,362 - +0.3 1,069,362 - +0.6 1,069,414 + AMB_RS05170 0.14 -0.0 1,069,414 + AMB_RS05170 0.14 -0.1 1,069,415 - AMB_RS05170 0.14 +0.4 1,069,669 - -0.1 1,069,704 + -0.3 1,069,834 + AMB_RS05175 0.16 -0.4 1,069,840 + AMB_RS05175 0.17 -0.5 1,070,026 + AMB_RS05175 0.61 -0.4 1,070,046 - AMB_RS05175 0.65 +0.9 1,070,236 - -0.6 1,070,301 + -1.0 1,070,301 + -2.3 1,070,301 + +0.4 1,070,301 + -0.4 1,070,301 + -0.3 1,070,301 + -0.5 1,070,302 - -0.4 1,070,302 - -0.2 1,070,302 - +0.4 1,070,303 + -1.9 1,070,303 + +0.4 1,070,303 + -0.7 1,070,303 + -2.4 1,070,303 + -1.3 1,070,303 + +0.4 1,070,304 - -0.6 1,070,304 - -1.2 1,070,304 - -0.5 1,070,304 - +0.4 1,070,304 - -2.6 1,070,593 + AMB_RS05180 0.69 -0.3 1,070,593 + AMB_RS05180 0.69 +0.5 1,070,593 + AMB_RS05180 0.69 +1.3
Or see this region's nucleotide sequence