Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05145 and AMB_RS05150 are separated by 58 nucleotides AMB_RS05150 and AMB_RS05155 are separated by 47 nucleotides AMB_RS05155 and AMB_RS05160 overlap by 4 nucleotides AMB_RS05160 and AMB_RS05165 overlap by 4 nucleotides
AMB_RS05145: AMB_RS05145 - hypothetical protein, at 1,065,444 to 1,066,400
_RS05145
AMB_RS05150: AMB_RS05150 - trypsin-like serine protease, at 1,066,459 to 1,067,316
_RS05150
AMB_RS05155: AMB_RS05155 - LemA family protein, at 1,067,364 to 1,068,182
_RS05155
AMB_RS05160: AMB_RS05160 - DNA-binding protein, at 1,068,179 to 1,068,433
_RS05160
AMB_RS05165: AMB_RS05165 - cation transporter, at 1,068,430 to 1,069,320
_RS05165
Position (kb)
1067
1068
1069 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1067.314 kb on + strand at 1067.315 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.321 kb on - strand at 1067.341 kb on - strand at 1067.341 kb on - strand at 1067.341 kb on - strand at 1067.352 kb on - strand at 1067.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 1,067,314 + +0.1 1,067,315 - +0.2 1,067,321 - +0.9 1,067,321 - -0.3 1,067,321 - +0.4 1,067,321 - -2.2 1,067,321 - -0.0 1,067,321 - +0.6 1,067,321 - -1.2 1,067,341 - -0.4 1,067,341 - +0.1 1,067,341 - -1.9 1,067,352 - -1.8 1,067,352 - -3.2
Or see this region's nucleotide sequence