Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04680 and AMB_RS04685 are separated by 169 nucleotides AMB_RS04685 and AMB_RS04690 are separated by 150 nucleotides
AMB_RS04680: AMB_RS04680 - transposase, at 979,020 to 979,286
_RS04680
AMB_RS04685: AMB_RS04685 - DNA-binding response regulator, at 979,456 to 980,070
_RS04685
AMB_RS04690: AMB_RS04690 - hypothetical protein, at 980,221 to 980,460
_RS04690
Position (kb) 980
981 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.315 kb on + strand at 979.316 kb on - strand at 979.330 kb on - strand at 979.376 kb on - strand at 979.445 kb on + strand at 979.445 kb on + strand at 979.457 kb on + strand at 979.483 kb on - strand at 979.504 kb on + strand at 979.505 kb on - strand at 979.507 kb on + strand at 979.508 kb on - strand at 979.558 kb on - strand, within AMB_RS04685 at 979.561 kb on + strand, within AMB_RS04685 at 979.561 kb on + strand, within AMB_RS04685 at 979.562 kb on - strand, within AMB_RS04685 at 979.588 kb on + strand, within AMB_RS04685 at 979.629 kb on + strand, within AMB_RS04685 at 979.629 kb on + strand, within AMB_RS04685 at 979.630 kb on - strand, within AMB_RS04685 at 979.697 kb on + strand, within AMB_RS04685 at 979.697 kb on + strand, within AMB_RS04685 at 979.724 kb on - strand, within AMB_RS04685 at 979.759 kb on - strand, within AMB_RS04685 at 979.788 kb on + strand, within AMB_RS04685 at 979.831 kb on + strand, within AMB_RS04685 at 980.003 kb on + strand, within AMB_RS04685 at 980.003 kb on + strand, within AMB_RS04685 at 980.058 kb on + strand at 980.058 kb on + strand at 980.059 kb on - strand at 980.108 kb on - strand at 980.191 kb on + strand at 980.191 kb on + strand at 980.191 kb on + strand at 980.193 kb on + strand at 980.307 kb on - strand, within AMB_RS04690 at 980.398 kb on - strand, within AMB_RS04690 at 980.406 kb on - strand, within AMB_RS04690 at 980.465 kb on + strand at 980.465 kb on + strand at 980.465 kb on + strand at 980.466 kb on - strand at 980.466 kb on - strand at 980.507 kb on + strand at 980.507 kb on + strand at 980.533 kb on + strand at 980.533 kb on + strand at 980.741 kb on + strand at 980.742 kb on - strand at 980.742 kb on - strand at 980.742 kb on - strand at 980.742 kb on - strand at 980.808 kb on + strand at 980.822 kb on + strand at 980.822 kb on + strand at 980.823 kb on - strand at 980.858 kb on + strand at 980.935 kb on + strand at 980.936 kb on - strand at 981.068 kb on - strand at 981.092 kb on + strand at 981.092 kb on + strand at 981.173 kb on - strand at 981.229 kb on + strand at 981.355 kb on - strand at 981.371 kb on + strand at 981.371 kb on + strand at 981.371 kb on + strand at 981.372 kb on - strand at 981.400 kb on + strand at 981.401 kb on - strand at 981.416 kb on - strand at 981.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 979,286 + +0.7 979,286 + -0.4 979,286 + -0.2 979,286 + -0.4 979,286 + -1.2 979,286 + -0.7 979,286 + -0.4 979,286 + -0.4 979,287 - -1.1 979,287 - -0.5 979,287 - +0.4 979,287 - -0.9 979,287 - -0.3 979,315 + +0.1 979,316 - -0.5 979,330 - +0.0 979,376 - -1.0 979,445 + -0.5 979,445 + +0.9 979,457 + -1.0 979,483 - +0.0 979,504 + -0.4 979,505 - +0.4 979,507 + +0.5 979,508 - -0.2 979,558 - AMB_RS04685 0.17 -2.1 979,561 + AMB_RS04685 0.17 -0.7 979,561 + AMB_RS04685 0.17 -0.4 979,562 - AMB_RS04685 0.17 -0.7 979,588 + AMB_RS04685 0.21 -0.5 979,629 + AMB_RS04685 0.28 -0.4 979,629 + AMB_RS04685 0.28 +0.4 979,630 - AMB_RS04685 0.28 -0.6 979,697 + AMB_RS04685 0.39 +0.4 979,697 + AMB_RS04685 0.39 -1.2 979,724 - AMB_RS04685 0.44 -0.6 979,759 - AMB_RS04685 0.49 -1.2 979,788 + AMB_RS04685 0.54 +0.0 979,831 + AMB_RS04685 0.61 +0.1 980,003 + AMB_RS04685 0.89 +1.8 980,003 + AMB_RS04685 0.89 -1.3 980,058 + -0.3 980,058 + -0.5 980,059 - +0.0 980,108 - -0.4 980,191 + +0.3 980,191 + +0.6 980,191 + -0.1 980,193 + -0.6 980,307 - AMB_RS04690 0.36 -0.0 980,398 - AMB_RS04690 0.74 -2.0 980,406 - AMB_RS04690 0.77 -0.9 980,465 + -0.4 980,465 + -0.8 980,465 + +0.0 980,466 - -0.3 980,466 - +0.2 980,507 + -0.4 980,507 + -0.1 980,533 + -0.3 980,533 + -1.7 980,741 + -1.2 980,742 - -1.1 980,742 - -0.4 980,742 - -0.3 980,742 - -0.1 980,808 + -1.8 980,822 + -2.9 980,822 + -0.4 980,823 - +1.6 980,858 + -1.9 980,935 + -0.4 980,936 - -0.1 981,068 - -0.6 981,092 + +1.0 981,092 + -0.8 981,173 - -0.1 981,229 + +0.1 981,355 - -0.4 981,371 + -1.2 981,371 + -0.0 981,371 + +0.0 981,372 - +0.8 981,400 + -0.2 981,401 - -1.2 981,416 - +0.2 981,447 - +1.4
Or see this region's nucleotide sequence