Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03245 and AMB_RS03250 are separated by 154 nucleotides AMB_RS03250 and AMB_RS03255 are separated by 66 nucleotides AMB_RS03255 and AMB_RS03260 are separated by 15 nucleotides
AMB_RS03245: AMB_RS03245 - 2-polyprenylphenol 6-hydroxylase, at 678,420 to 679,961
_RS03245
AMB_RS03250: AMB_RS03250 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 680,116 to 680,874
_RS03250
AMB_RS03255: AMB_RS03255 - bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase, at 680,941 to 681,780
_RS03255
AMB_RS03260: AMB_RS03260 - hypothetical protein, at 681,796 to 682,155
_RS03260
Position (kb) 680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 680.120 kb on - strand at 680.120 kb on - strand at 680.120 kb on - strand at 680.120 kb on - strand at 680.325 kb on - strand, within AMB_RS03250 at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.928 kb on + strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.929 kb on - strand at 680.930 kb on + strand at 680.930 kb on + strand at 680.930 kb on + strand at 680.931 kb on - strand at 680.931 kb on - strand at 680.931 kb on - strand at 680.931 kb on - strand at 681.116 kb on - strand, within AMB_RS03255 at 681.116 kb on - strand, within AMB_RS03255 at 681.116 kb on - strand, within AMB_RS03255 at 681.116 kb on - strand, within AMB_RS03255 at 681.116 kb on - strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.466 kb on + strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.467 kb on - strand, within AMB_RS03255 at 681.641 kb on - strand, within AMB_RS03255 at 681.664 kb on + strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.665 kb on - strand, within AMB_RS03255 at 681.757 kb on + strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.758 kb on - strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.784 kb on + strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.785 kb on - strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.786 kb on + strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand at 681.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 680,120 - -2.4 680,120 - +0.4 680,120 - -2.9 680,120 - -1.2 680,325 - AMB_RS03250 0.28 +0.4 680,928 + -1.4 680,928 + -2.8 680,928 + +0.1 680,928 + +0.2 680,928 + -0.2 680,929 - -0.0 680,929 - -2.2 680,929 - -0.1 680,929 - -1.2 680,929 - -0.5 680,929 - -0.3 680,929 - +1.4 680,929 - -0.9 680,930 + -0.8 680,930 + -0.9 680,930 + +0.6 680,931 - +0.6 680,931 - -0.7 680,931 - -0.3 680,931 - -0.1 681,116 - AMB_RS03255 0.21 +0.1 681,116 - AMB_RS03255 0.21 +0.1 681,116 - AMB_RS03255 0.21 -0.1 681,116 - AMB_RS03255 0.21 -0.2 681,116 - AMB_RS03255 0.21 +0.3 681,466 + AMB_RS03255 0.62 +0.2 681,466 + AMB_RS03255 0.62 -0.2 681,466 + AMB_RS03255 0.62 -1.1 681,466 + AMB_RS03255 0.62 +0.8 681,466 + AMB_RS03255 0.62 -0.6 681,466 + AMB_RS03255 0.62 -0.1 681,466 + AMB_RS03255 0.62 -0.2 681,467 - AMB_RS03255 0.63 -0.7 681,467 - AMB_RS03255 0.63 -0.1 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 +1.5 681,467 - AMB_RS03255 0.63 -0.0 681,467 - AMB_RS03255 0.63 -0.4 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 -0.2 681,467 - AMB_RS03255 0.63 +0.1 681,467 - AMB_RS03255 0.63 -0.1 681,467 - AMB_RS03255 0.63 -0.7 681,467 - AMB_RS03255 0.63 +1.4 681,467 - AMB_RS03255 0.63 +0.0 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 -0.9 681,467 - AMB_RS03255 0.63 -0.6 681,467 - AMB_RS03255 0.63 +0.1 681,641 - AMB_RS03255 0.83 +0.4 681,664 + AMB_RS03255 0.86 -1.1 681,665 - AMB_RS03255 0.86 +0.1 681,665 - AMB_RS03255 0.86 -0.7 681,665 - AMB_RS03255 0.86 -0.2 681,757 + +0.9 681,758 - -2.0 681,758 - +1.0 681,758 - -0.3 681,758 - +0.3 681,784 + +0.0 681,784 + +1.3 681,784 + +0.3 681,784 + +0.2 681,784 + +1.4 681,785 - -0.6 681,785 - +0.1 681,785 - -2.1 681,785 - +0.2 681,785 - -2.2 681,785 - -0.4 681,785 - +1.4 681,786 + +0.3 681,786 + -1.1 681,786 + -0.3 681,786 + -0.2 681,787 - -0.5 681,787 - +0.1 681,787 - +0.7 681,787 - -0.8 681,787 - -0.3 681,787 - +0.5
Or see this region's nucleotide sequence