Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01220 and AMB_RS01225 overlap by 44 nucleotides AMB_RS01225 and AMB_RS01230 overlap by 1 nucleotides AMB_RS01230 and AMB_RS01235 overlap by 4 nucleotides AMB_RS01235 and AMB_RS01240 are separated by 101 nucleotides
AMB_RS01220: AMB_RS01220 - hypothetical protein, at 272,248 to 273,783
_RS01220
AMB_RS01225: AMB_RS01225 - tRNA pseudouridine(38-40) synthase TruA, at 273,740 to 274,510
_RS01225
AMB_RS01230: AMB_RS01230 - methionyl-tRNA formyltransferase, at 274,510 to 275,427
_RS01230
AMB_RS01235: AMB_RS01235 - peptide deformylase, at 275,424 to 275,936
_RS01235
AMB_RS01240: AMB_RS01240 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 276,038 to 276,796
_RS01240
Position (kb) 274
275
276 Strain fitness (log2 ratio) -1
0
1 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.517 kb on + strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.518 kb on - strand, within AMB_RS01220 at 273.637 kb on - strand at 273.637 kb on - strand at 273.724 kb on + strand at 273.724 kb on + strand at 273.900 kb on - strand, within AMB_RS01225 at 274.179 kb on + strand, within AMB_RS01225 at 274.179 kb on + strand, within AMB_RS01225 at 274.269 kb on + strand, within AMB_RS01225 at 274.269 kb on + strand, within AMB_RS01225 at 274.511 kb on + strand at 275.973 kb on - strand at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 273,517 + AMB_RS01220 0.83 -0.1 273,517 + AMB_RS01220 0.83 -0.7 273,517 + AMB_RS01220 0.83 -0.2 273,517 + AMB_RS01220 0.83 +0.3 273,517 + AMB_RS01220 0.83 +0.6 273,517 + AMB_RS01220 0.83 -0.2 273,517 + AMB_RS01220 0.83 +0.6 273,517 + AMB_RS01220 0.83 -0.8 273,517 + AMB_RS01220 0.83 -0.7 273,518 - AMB_RS01220 0.83 +0.5 273,518 - AMB_RS01220 0.83 -0.1 273,518 - AMB_RS01220 0.83 -0.6 273,518 - AMB_RS01220 0.83 -0.0 273,518 - AMB_RS01220 0.83 -0.2 273,518 - AMB_RS01220 0.83 +0.5 273,518 - AMB_RS01220 0.83 -1.2 273,518 - AMB_RS01220 0.83 +0.1 273,518 - AMB_RS01220 0.83 +0.3 273,637 - -0.4 273,637 - -1.1 273,724 + -0.1 273,724 + +0.5 273,900 - AMB_RS01225 0.21 +1.5 274,179 + AMB_RS01225 0.57 +0.5 274,179 + AMB_RS01225 0.57 -0.5 274,269 + AMB_RS01225 0.69 +0.0 274,269 + AMB_RS01225 0.69 +0.2 274,511 + +0.6 275,973 - -0.1 276,134 + AMB_RS01240 0.13 -1.0 276,134 + AMB_RS01240 0.13 -1.0 276,134 + AMB_RS01240 0.13 -0.5 276,134 + AMB_RS01240 0.13 -1.5 276,134 + AMB_RS01240 0.13 -0.3 276,134 + AMB_RS01240 0.13 -1.7 276,135 - AMB_RS01240 0.13 -0.0 276,135 - AMB_RS01240 0.13 +1.5 276,135 - AMB_RS01240 0.13 -0.6 276,135 - AMB_RS01240 0.13 +0.0 276,135 - AMB_RS01240 0.13 -1.5
Or see this region's nucleotide sequence