Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01005 and AMB_RS01010 are separated by 55 nucleotides AMB_RS01010 and AMB_RS01015 are separated by 5 nucleotides AMB_RS01015 and AMB_RS01020 overlap by 1 nucleotides AMB_RS01020 and AMB_RS01025 are separated by 163 nucleotides
AMB_RS01005: AMB_RS01005 - cysteine synthase A, at 224,260 to 225,219
_RS01005
AMB_RS01010: AMB_RS01010 - Rrf2 family transcriptional regulator, at 225,275 to 225,730
_RS01010
AMB_RS01015: AMB_RS01015 - dUTP diphosphatase, at 225,736 to 226,191
_RS01015
AMB_RS01020: AMB_RS01020 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 226,191 to 227,393
_RS01020
AMB_RS01025: AMB_RS01025 - molecular chaperone GroEL, at 227,557 to 229,215
_RS01025
Position (kb) 226
227
228 Strain fitness (log2 ratio) -2
-1
0
1 at 225.277 kb on - strand at 225.327 kb on + strand, within AMB_RS01010 at 225.327 kb on + strand, within AMB_RS01010 at 225.607 kb on - strand, within AMB_RS01010 at 225.738 kb on - strand at 226.014 kb on - strand, within AMB_RS01015 at 226.133 kb on + strand, within AMB_RS01015 at 226.134 kb on - strand, within AMB_RS01015 at 227.435 kb on - strand at 227.558 kb on + strand at 227.558 kb on + strand at 227.558 kb on + strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 225,277 - -1.0 225,327 + AMB_RS01010 0.11 -1.3 225,327 + AMB_RS01010 0.11 -1.0 225,607 - AMB_RS01010 0.73 +0.0 225,738 - -0.7 226,014 - AMB_RS01015 0.61 -0.8 226,133 + AMB_RS01015 0.87 -0.0 226,134 - AMB_RS01015 0.87 -1.3 227,435 - +0.2 227,558 + +0.2 227,558 + -1.1 227,558 + -0.4 227,559 - -0.4 227,559 - -0.5 227,559 - -1.5 227,559 - -2.5 227,559 - -0.9 227,559 - -2.2 227,559 - -2.3 227,559 - -1.6 227,559 - -0.7 227,559 - +0.3 227,559 - -0.0 227,559 - -0.3 227,559 - -2.1 227,559 - -0.1
Or see this region's nucleotide sequence