Experiment: 150_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00410 and AMB_RS00415 are separated by 3 nucleotides AMB_RS00415 and AMB_RS00420 overlap by 76 nucleotides AMB_RS00420 and AMB_RS23090 overlap by 4 nucleotides AMB_RS23090 and AMB_RS00430 overlap by 4 nucleotides
AMB_RS00410: AMB_RS00410 - class I SAM-dependent methyltransferase, at 89,363 to 90,535
_RS00410
AMB_RS00415: AMB_RS00415 - histidinol-phosphatase, at 90,539 to 91,252
_RS00415
AMB_RS00420: AMB_RS00420 - hypothetical protein, at 91,177 to 92,289
_RS00420
AMB_RS23090: AMB_RS23090 - NAD(P)-dependent oxidoreductase, at 92,286 to 93,182
_RS23090
AMB_RS00430: AMB_RS00430 - CBS domain-containing protein, at 93,179 to 94,228
_RS00430
Position (kb) 91
92
93 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 90.289 kb on + strand, within AMB_RS00410 at 90.419 kb on + strand at 90.420 kb on - strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.440 kb on + strand at 90.441 kb on - strand at 90.928 kb on + strand, within AMB_RS00415 at 92.679 kb on + strand, within AMB_RS23090 at 92.679 kb on + strand, within AMB_RS23090 at 92.679 kb on + strand, within AMB_RS23090 at 92.679 kb on + strand, within AMB_RS23090 at 92.679 kb on + strand, within AMB_RS23090 at 92.780 kb on + strand, within AMB_RS23090 at 92.780 kb on + strand, within AMB_RS23090
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_2 remove 90,289 + AMB_RS00410 0.79 +0.2 90,419 + +0.0 90,420 - -0.5 90,440 + +0.8 90,440 + -1.1 90,440 + +2.0 90,440 + +1.5 90,440 + +0.3 90,440 + -0.2 90,440 + -0.6 90,440 + -0.1 90,440 + -1.4 90,440 + -0.4 90,440 + +0.3 90,440 + +0.7 90,440 + -1.5 90,440 + -0.5 90,441 - -1.9 90,928 + AMB_RS00415 0.54 +0.8 92,679 + AMB_RS23090 0.44 -1.1 92,679 + AMB_RS23090 0.44 -3.8 92,679 + AMB_RS23090 0.44 -0.5 92,679 + AMB_RS23090 0.44 -1.9 92,679 + AMB_RS23090 0.44 -0.3 92,780 + AMB_RS23090 0.55 -2.9 92,780 + AMB_RS23090 0.55 -1.3
Or see this region's nucleotide sequence