Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS06540 and ABIE40_RS06545 are separated by 13 nucleotides ABIE40_RS06545 and ABIE40_RS06550 overlap by 1 nucleotides ABIE40_RS06550 and ABIE40_RS06555 are separated by 58 nucleotides ABIE40_RS06555 and ABIE40_RS06560 overlap by 4 nucleotides
ABIE40_RS06540: ABIE40_RS06540 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 1,280,924 to 1,282,094
_RS06540
ABIE40_RS06545: ABIE40_RS06545 - type II toxin-antitoxin system VapC family toxin, at 1,282,108 to 1,282,509
_RS06545
ABIE40_RS06550: ABIE40_RS06550 - type II toxin-antitoxin system prevent-host-death family antitoxin, at 1,282,509 to 1,282,745
_RS06550
ABIE40_RS06555: ABIE40_RS06555 - phosphoribosylglycinamide formyltransferase, at 1,282,804 to 1,283,466
_RS06555
ABIE40_RS06560: ABIE40_RS06560 - phosphoribosylformylglycinamidine cyclo-ligase, at 1,283,463 to 1,284,536
_RS06560
Position (kb)
1282
1283
1284 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.050 kb on + strand at 1282.051 kb on - strand at 1282.053 kb on - strand at 1282.053 kb on - strand at 1282.061 kb on + strand at 1282.062 kb on - strand at 1282.092 kb on + strand at 1282.103 kb on + strand at 1282.103 kb on + strand at 1282.103 kb on + strand at 1282.103 kb on + strand at 1282.104 kb on - strand at 1282.205 kb on + strand, within ABIE40_RS06545 at 1282.206 kb on - strand, within ABIE40_RS06545 at 1282.230 kb on + strand, within ABIE40_RS06545 at 1282.231 kb on - strand, within ABIE40_RS06545 at 1282.437 kb on + strand, within ABIE40_RS06545 at 1282.437 kb on + strand, within ABIE40_RS06545 at 1282.437 kb on + strand, within ABIE40_RS06545 at 1282.438 kb on - strand, within ABIE40_RS06545 at 1282.438 kb on - strand, within ABIE40_RS06545 at 1282.438 kb on - strand, within ABIE40_RS06545 at 1282.438 kb on - strand, within ABIE40_RS06545 at 1282.452 kb on + strand, within ABIE40_RS06545 at 1282.453 kb on - strand, within ABIE40_RS06545 at 1282.498 kb on + strand at 1282.498 kb on + strand at 1282.498 kb on + strand at 1282.501 kb on + strand at 1282.501 kb on + strand at 1282.501 kb on + strand at 1282.501 kb on + strand at 1282.501 kb on + strand at 1282.502 kb on - strand at 1282.502 kb on - strand at 1282.502 kb on - strand at 1282.563 kb on - strand, within ABIE40_RS06550 at 1282.589 kb on + strand, within ABIE40_RS06550 at 1282.589 kb on + strand, within ABIE40_RS06550 at 1282.590 kb on - strand, within ABIE40_RS06550 at 1282.590 kb on - strand, within ABIE40_RS06550 at 1282.670 kb on + strand, within ABIE40_RS06550 at 1282.670 kb on + strand, within ABIE40_RS06550 at 1282.671 kb on - strand, within ABIE40_RS06550 at 1282.725 kb on + strand at 1282.725 kb on + strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.726 kb on - strand at 1282.738 kb on - strand at 1282.922 kb on + strand, within ABIE40_RS06555 at 1282.991 kb on - strand, within ABIE40_RS06555 at 1283.270 kb on + strand, within ABIE40_RS06555 at 1283.467 kb on - strand at 1283.477 kb on + strand at 1283.683 kb on - strand, within ABIE40_RS06560 at 1283.836 kb on + strand, within ABIE40_RS06560 at 1283.836 kb on + strand, within ABIE40_RS06560 at 1283.836 kb on + strand, within ABIE40_RS06560 at 1283.836 kb on + strand, within ABIE40_RS06560 at 1283.837 kb on - strand, within ABIE40_RS06560 at 1283.837 kb on - strand, within ABIE40_RS06560 at 1283.938 kb on - strand, within ABIE40_RS06560 at 1284.082 kb on + strand, within ABIE40_RS06560 at 1284.082 kb on + strand, within ABIE40_RS06560 at 1284.083 kb on - strand, within ABIE40_RS06560 at 1284.083 kb on - strand, within ABIE40_RS06560 at 1284.083 kb on - strand, within ABIE40_RS06560 at 1284.083 kb on - strand, within ABIE40_RS06560 at 1284.083 kb on - strand, within ABIE40_RS06560 at 1284.284 kb on + strand, within ABIE40_RS06560 at 1284.293 kb on - strand, within ABIE40_RS06560
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 1,282,050 + -0.7 1,282,050 + -0.4 1,282,050 + +1.0 1,282,050 + +0.4 1,282,050 + -0.8 1,282,050 + +0.3 1,282,050 + -1.2 1,282,050 + -1.9 1,282,050 + +1.1 1,282,051 - +0.2 1,282,053 - +1.8 1,282,053 - -1.1 1,282,061 + -0.1 1,282,062 - -0.3 1,282,092 + -1.9 1,282,103 + -1.5 1,282,103 + -2.7 1,282,103 + -0.1 1,282,103 + -0.8 1,282,104 - -0.1 1,282,205 + ABIE40_RS06545 0.24 -0.6 1,282,206 - ABIE40_RS06545 0.24 -0.5 1,282,230 + ABIE40_RS06545 0.30 +0.9 1,282,231 - ABIE40_RS06545 0.31 -0.3 1,282,437 + ABIE40_RS06545 0.82 +0.5 1,282,437 + ABIE40_RS06545 0.82 -0.6 1,282,437 + ABIE40_RS06545 0.82 -1.3 1,282,438 - ABIE40_RS06545 0.82 -0.4 1,282,438 - ABIE40_RS06545 0.82 +0.3 1,282,438 - ABIE40_RS06545 0.82 -0.1 1,282,438 - ABIE40_RS06545 0.82 -0.6 1,282,452 + ABIE40_RS06545 0.86 +0.2 1,282,453 - ABIE40_RS06545 0.86 -0.4 1,282,498 + -0.7 1,282,498 + -0.7 1,282,498 + +1.3 1,282,501 + +0.7 1,282,501 + -0.2 1,282,501 + +0.6 1,282,501 + -0.9 1,282,501 + -0.7 1,282,502 - +0.8 1,282,502 - -0.1 1,282,502 - +1.3 1,282,563 - ABIE40_RS06550 0.23 +0.8 1,282,589 + ABIE40_RS06550 0.34 -0.2 1,282,589 + ABIE40_RS06550 0.34 -0.2 1,282,590 - ABIE40_RS06550 0.34 +0.6 1,282,590 - ABIE40_RS06550 0.34 -0.6 1,282,670 + ABIE40_RS06550 0.68 -1.7 1,282,670 + ABIE40_RS06550 0.68 +0.2 1,282,671 - ABIE40_RS06550 0.68 +0.1 1,282,725 + -0.1 1,282,725 + -0.5 1,282,726 - -0.0 1,282,726 - -3.1 1,282,726 - -0.3 1,282,726 - -0.0 1,282,726 - -1.2 1,282,726 - +0.0 1,282,726 - -0.7 1,282,726 - -0.6 1,282,738 - -0.6 1,282,922 + ABIE40_RS06555 0.18 -2.2 1,282,991 - ABIE40_RS06555 0.28 -3.3 1,283,270 + ABIE40_RS06555 0.70 -2.9 1,283,467 - -1.1 1,283,477 + -2.8 1,283,683 - ABIE40_RS06560 0.20 -2.1 1,283,836 + ABIE40_RS06560 0.35 -2.7 1,283,836 + ABIE40_RS06560 0.35 -1.6 1,283,836 + ABIE40_RS06560 0.35 -1.1 1,283,836 + ABIE40_RS06560 0.35 -1.6 1,283,837 - ABIE40_RS06560 0.35 -1.6 1,283,837 - ABIE40_RS06560 0.35 -2.1 1,283,938 - ABIE40_RS06560 0.44 -2.9 1,284,082 + ABIE40_RS06560 0.58 -2.8 1,284,082 + ABIE40_RS06560 0.58 -2.9 1,284,083 - ABIE40_RS06560 0.58 -1.3 1,284,083 - ABIE40_RS06560 0.58 -4.4 1,284,083 - ABIE40_RS06560 0.58 -3.3 1,284,083 - ABIE40_RS06560 0.58 -1.9 1,284,083 - ABIE40_RS06560 0.58 -1.9 1,284,284 + ABIE40_RS06560 0.76 -2.3 1,284,293 - ABIE40_RS06560 0.77 -0.7
Or see this region's nucleotide sequence