Experiment: D-Serine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmA and btuB are separated by 368 nucleotides btuB and murI overlap by 56 nucleotides
b3965: trmA - tRNA (uracil-5-)-methyltransferase (NCBI), at 4,160,193 to 4,161,293
trmA
b3966: btuB - vitamin B12/cobalamin outer membrane transporter (NCBI), at 4,161,662 to 4,163,506
btuB
b3967: murI - glutamate racemase, required for biosynthesis of D-glutamate and peptidoglycan (VIMSS), at 4,163,451 to 4,164,308
murI
Position (kb)
4161
4162
4163
4164 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4160.673 kb on + strand, within trmA at 4160.673 kb on + strand, within trmA at 4160.686 kb on + strand, within trmA at 4160.687 kb on - strand, within trmA at 4160.687 kb on - strand, within trmA at 4160.734 kb on - strand, within trmA at 4160.751 kb on - strand, within trmA at 4160.767 kb on - strand, within trmA at 4160.767 kb on - strand, within trmA at 4160.781 kb on + strand, within trmA at 4160.781 kb on - strand, within trmA at 4160.799 kb on - strand, within trmA at 4160.877 kb on - strand, within trmA at 4160.882 kb on - strand, within trmA at 4160.914 kb on + strand, within trmA at 4160.921 kb on + strand, within trmA at 4160.921 kb on - strand, within trmA at 4160.922 kb on + strand, within trmA at 4160.930 kb on - strand, within trmA at 4160.930 kb on - strand, within trmA at 4160.952 kb on - strand, within trmA at 4160.954 kb on + strand, within trmA at 4160.954 kb on + strand, within trmA at 4160.962 kb on - strand, within trmA at 4160.969 kb on - strand, within trmA at 4160.969 kb on - strand, within trmA at 4160.983 kb on - strand, within trmA at 4160.993 kb on + strand, within trmA at 4161.011 kb on - strand, within trmA at 4161.011 kb on - strand, within trmA at 4161.013 kb on + strand, within trmA at 4161.013 kb on + strand, within trmA at 4161.021 kb on - strand, within trmA at 4161.021 kb on - strand, within trmA at 4161.055 kb on - strand, within trmA at 4161.103 kb on + strand, within trmA at 4161.139 kb on + strand, within trmA at 4161.149 kb on - strand, within trmA at 4161.161 kb on + strand, within trmA at 4161.164 kb on - strand, within trmA at 4161.215 kb on + strand at 4161.223 kb on - strand at 4161.259 kb on + strand at 4161.264 kb on - strand at 4161.327 kb on + strand at 4161.337 kb on + strand at 4161.390 kb on - strand at 4161.436 kb on - strand at 4161.446 kb on + strand at 4161.448 kb on - strand at 4161.448 kb on - strand at 4161.537 kb on - strand at 4161.537 kb on - strand at 4161.538 kb on - strand at 4161.548 kb on - strand at 4161.548 kb on - strand at 4161.564 kb on + strand at 4161.564 kb on + strand at 4161.572 kb on - strand at 4161.609 kb on + strand at 4161.618 kb on - strand at 4161.618 kb on - strand at 4161.622 kb on - strand at 4161.622 kb on - strand at 4161.722 kb on - strand at 4161.741 kb on + strand at 4161.741 kb on + strand at 4161.783 kb on - strand at 4161.783 kb on - strand at 4161.791 kb on + strand at 4161.794 kb on - strand at 4161.826 kb on - strand at 4161.826 kb on - strand at 4161.836 kb on - strand at 4161.907 kb on + strand, within btuB at 4161.949 kb on + strand, within btuB at 4161.949 kb on + strand, within btuB at 4161.994 kb on - strand, within btuB at 4161.994 kb on - strand, within btuB at 4161.996 kb on - strand, within btuB at 4162.005 kb on + strand, within btuB at 4162.013 kb on - strand, within btuB at 4162.017 kb on + strand, within btuB at 4162.017 kb on + strand, within btuB at 4162.023 kb on - strand, within btuB at 4162.028 kb on - strand, within btuB at 4162.050 kb on + strand, within btuB at 4162.053 kb on - strand, within btuB at 4162.070 kb on + strand, within btuB at 4162.078 kb on - strand, within btuB at 4162.107 kb on - strand, within btuB at 4162.145 kb on + strand, within btuB at 4162.148 kb on + strand, within btuB at 4162.174 kb on + strand, within btuB at 4162.182 kb on + strand, within btuB at 4162.182 kb on + strand, within btuB at 4162.190 kb on + strand, within btuB at 4162.190 kb on - strand, within btuB at 4162.190 kb on - strand, within btuB at 4162.202 kb on + strand, within btuB at 4162.237 kb on + strand, within btuB at 4162.265 kb on + strand, within btuB at 4162.270 kb on + strand, within btuB at 4162.278 kb on - strand, within btuB at 4162.334 kb on + strand, within btuB at 4162.385 kb on + strand, within btuB at 4162.388 kb on - strand, within btuB at 4162.388 kb on - strand, within btuB at 4162.414 kb on + strand, within btuB at 4162.436 kb on + strand, within btuB at 4162.514 kb on + strand, within btuB at 4162.524 kb on + strand, within btuB at 4162.532 kb on - strand, within btuB at 4162.537 kb on - strand, within btuB at 4162.541 kb on + strand, within btuB at 4162.541 kb on + strand, within btuB at 4162.591 kb on + strand, within btuB at 4162.596 kb on + strand, within btuB at 4162.603 kb on + strand, within btuB at 4162.603 kb on + strand, within btuB at 4162.603 kb on + strand, within btuB at 4162.603 kb on + strand, within btuB at 4162.658 kb on - strand, within btuB at 4162.661 kb on + strand, within btuB at 4162.669 kb on - strand, within btuB at 4162.669 kb on - strand, within btuB at 4162.670 kb on - strand, within btuB at 4162.701 kb on - strand, within btuB at 4162.701 kb on - strand, within btuB at 4162.706 kb on + strand, within btuB at 4162.706 kb on + strand, within btuB at 4162.706 kb on + strand, within btuB at 4162.716 kb on + strand, within btuB at 4162.721 kb on + strand, within btuB at 4162.721 kb on + strand, within btuB at 4162.726 kb on + strand, within btuB at 4162.726 kb on + strand, within btuB at 4162.734 kb on - strand, within btuB at 4162.734 kb on - strand, within btuB at 4162.734 kb on - strand, within btuB at 4162.741 kb on + strand, within btuB at 4162.741 kb on + strand, within btuB at 4162.776 kb on + strand, within btuB at 4162.785 kb on + strand, within btuB at 4162.785 kb on + strand, within btuB at 4162.785 kb on + strand, within btuB at 4162.822 kb on + strand, within btuB at 4162.822 kb on + strand, within btuB at 4162.830 kb on - strand, within btuB at 4162.830 kb on - strand, within btuB at 4162.866 kb on + strand, within btuB at 4162.885 kb on + strand, within btuB at 4162.918 kb on - strand, within btuB at 4162.924 kb on + strand, within btuB at 4162.924 kb on + strand, within btuB at 4162.985 kb on + strand, within btuB at 4162.985 kb on + strand, within btuB at 4163.011 kb on - strand, within btuB at 4163.109 kb on - strand, within btuB at 4163.141 kb on + strand, within btuB at 4163.141 kb on + strand, within btuB at 4163.146 kb on + strand, within btuB at 4163.148 kb on + strand, within btuB at 4163.156 kb on - strand, within btuB at 4163.168 kb on - strand, within btuB at 4163.212 kb on - strand, within btuB at 4163.212 kb on - strand, within btuB at 4163.214 kb on + strand, within btuB at 4163.214 kb on + strand, within btuB at 4163.269 kb on + strand, within btuB at 4163.269 kb on + strand, within btuB at 4163.272 kb on - strand, within btuB at 4163.272 kb on - strand, within btuB at 4163.317 kb on + strand, within btuB at 4163.357 kb on - strand at 4163.357 kb on - strand at 4163.357 kb on - strand at 4163.388 kb on + strand at 4163.388 kb on + strand at 4163.447 kb on + strand at 4163.447 kb on + strand at 4163.455 kb on - strand at 4163.455 kb on - strand at 4163.455 kb on - strand at 4163.455 kb on - strand at 4163.455 kb on - strand at 4163.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Serine (C) remove 4,160,673 + trmA b3965 0.44 +1.1 4,160,673 + trmA b3965 0.44 +0.2 4,160,686 + trmA b3965 0.45 -1.4 4,160,687 - trmA b3965 0.45 +0.4 4,160,687 - trmA b3965 0.45 -0.4 4,160,734 - trmA b3965 0.49 -0.2 4,160,751 - trmA b3965 0.51 -0.6 4,160,767 - trmA b3965 0.52 +0.7 4,160,767 - trmA b3965 0.52 +1.1 4,160,781 + trmA b3965 0.53 -1.2 4,160,781 - trmA b3965 0.53 +0.5 4,160,799 - trmA b3965 0.55 -0.7 4,160,877 - trmA b3965 0.62 -0.8 4,160,882 - trmA b3965 0.63 -0.6 4,160,914 + trmA b3965 0.65 -0.7 4,160,921 + trmA b3965 0.66 -0.2 4,160,921 - trmA b3965 0.66 +0.1 4,160,922 + trmA b3965 0.66 +0.6 4,160,930 - trmA b3965 0.67 -0.8 4,160,930 - trmA b3965 0.67 +1.3 4,160,952 - trmA b3965 0.69 -0.4 4,160,954 + trmA b3965 0.69 -1.2 4,160,954 + trmA b3965 0.69 -0.3 4,160,962 - trmA b3965 0.70 -0.4 4,160,969 - trmA b3965 0.70 -0.6 4,160,969 - trmA b3965 0.70 +0.3 4,160,983 - trmA b3965 0.72 +0.7 4,160,993 + trmA b3965 0.73 -0.2 4,161,011 - trmA b3965 0.74 -1.0 4,161,011 - trmA b3965 0.74 -0.1 4,161,013 + trmA b3965 0.74 +0.4 4,161,013 + trmA b3965 0.74 -0.4 4,161,021 - trmA b3965 0.75 +0.1 4,161,021 - trmA b3965 0.75 -0.7 4,161,055 - trmA b3965 0.78 +0.1 4,161,103 + trmA b3965 0.83 -0.9 4,161,139 + trmA b3965 0.86 +0.1 4,161,149 - trmA b3965 0.87 -0.2 4,161,161 + trmA b3965 0.88 +0.1 4,161,164 - trmA b3965 0.88 +0.4 4,161,215 + -0.5 4,161,223 - +0.1 4,161,259 + +0.3 4,161,264 - +0.8 4,161,327 + +0.5 4,161,337 + -0.1 4,161,390 - +0.1 4,161,436 - -1.0 4,161,446 + -0.5 4,161,448 - -1.5 4,161,448 - +0.2 4,161,537 - -1.4 4,161,537 - -0.9 4,161,538 - -1.5 4,161,548 - +0.5 4,161,548 - -0.5 4,161,564 + -1.2 4,161,564 + -0.2 4,161,572 - -0.7 4,161,609 + +0.1 4,161,618 - -0.5 4,161,618 - -1.1 4,161,622 - +0.3 4,161,622 - -1.2 4,161,722 - -1.5 4,161,741 + -0.7 4,161,741 + -1.0 4,161,783 - -2.6 4,161,783 - -1.7 4,161,791 + -1.5 4,161,794 - +0.2 4,161,826 - -0.9 4,161,826 - -1.6 4,161,836 - -1.0 4,161,907 + btuB b3966 0.13 -1.4 4,161,949 + btuB b3966 0.16 -0.5 4,161,949 + btuB b3966 0.16 -1.4 4,161,994 - btuB b3966 0.18 -0.4 4,161,994 - btuB b3966 0.18 +0.5 4,161,996 - btuB b3966 0.18 -0.5 4,162,005 + btuB b3966 0.19 -1.0 4,162,013 - btuB b3966 0.19 -1.3 4,162,017 + btuB b3966 0.19 +0.8 4,162,017 + btuB b3966 0.19 +0.0 4,162,023 - btuB b3966 0.20 -1.0 4,162,028 - btuB b3966 0.20 -1.1 4,162,050 + btuB b3966 0.21 +0.9 4,162,053 - btuB b3966 0.21 -1.0 4,162,070 + btuB b3966 0.22 -1.4 4,162,078 - btuB b3966 0.23 -1.5 4,162,107 - btuB b3966 0.24 -0.2 4,162,145 + btuB b3966 0.26 -1.1 4,162,148 + btuB b3966 0.26 -0.9 4,162,174 + btuB b3966 0.28 -2.4 4,162,182 + btuB b3966 0.28 -1.6 4,162,182 + btuB b3966 0.28 -2.2 4,162,190 + btuB b3966 0.29 -1.7 4,162,190 - btuB b3966 0.29 -1.8 4,162,190 - btuB b3966 0.29 -0.9 4,162,202 + btuB b3966 0.29 -0.9 4,162,237 + btuB b3966 0.31 -1.7 4,162,265 + btuB b3966 0.33 -1.6 4,162,270 + btuB b3966 0.33 -2.4 4,162,278 - btuB b3966 0.33 -1.2 4,162,334 + btuB b3966 0.36 -0.4 4,162,385 + btuB b3966 0.39 -1.8 4,162,388 - btuB b3966 0.39 -1.2 4,162,388 - btuB b3966 0.39 -1.1 4,162,414 + btuB b3966 0.41 -2.2 4,162,436 + btuB b3966 0.42 -1.6 4,162,514 + btuB b3966 0.46 -1.1 4,162,524 + btuB b3966 0.47 -0.4 4,162,532 - btuB b3966 0.47 -1.5 4,162,537 - btuB b3966 0.47 -1.9 4,162,541 + btuB b3966 0.48 -0.8 4,162,541 + btuB b3966 0.48 -1.4 4,162,591 + btuB b3966 0.50 -0.8 4,162,596 + btuB b3966 0.51 -0.5 4,162,603 + btuB b3966 0.51 -0.0 4,162,603 + btuB b3966 0.51 -0.8 4,162,603 + btuB b3966 0.51 -0.2 4,162,603 + btuB b3966 0.51 +0.2 4,162,658 - btuB b3966 0.54 -1.4 4,162,661 + btuB b3966 0.54 -1.7 4,162,669 - btuB b3966 0.55 -1.9 4,162,669 - btuB b3966 0.55 -0.2 4,162,670 - btuB b3966 0.55 -1.1 4,162,701 - btuB b3966 0.56 -1.4 4,162,701 - btuB b3966 0.56 -0.2 4,162,706 + btuB b3966 0.57 -3.0 4,162,706 + btuB b3966 0.57 -1.1 4,162,706 + btuB b3966 0.57 -2.6 4,162,716 + btuB b3966 0.57 -1.2 4,162,721 + btuB b3966 0.57 -1.5 4,162,721 + btuB b3966 0.57 -1.0 4,162,726 + btuB b3966 0.58 -0.5 4,162,726 + btuB b3966 0.58 -1.9 4,162,734 - btuB b3966 0.58 -1.8 4,162,734 - btuB b3966 0.58 -0.2 4,162,734 - btuB b3966 0.58 -0.9 4,162,741 + btuB b3966 0.58 -1.6 4,162,741 + btuB b3966 0.58 -0.9 4,162,776 + btuB b3966 0.60 -1.3 4,162,785 + btuB b3966 0.61 -1.6 4,162,785 + btuB b3966 0.61 -1.2 4,162,785 + btuB b3966 0.61 -1.3 4,162,822 + btuB b3966 0.63 -1.2 4,162,822 + btuB b3966 0.63 -0.8 4,162,830 - btuB b3966 0.63 +0.2 4,162,830 - btuB b3966 0.63 -0.3 4,162,866 + btuB b3966 0.65 -0.2 4,162,885 + btuB b3966 0.66 -2.3 4,162,918 - btuB b3966 0.68 -0.4 4,162,924 + btuB b3966 0.68 -1.8 4,162,924 + btuB b3966 0.68 -1.2 4,162,985 + btuB b3966 0.72 -0.1 4,162,985 + btuB b3966 0.72 -1.3 4,163,011 - btuB b3966 0.73 -1.1 4,163,109 - btuB b3966 0.78 +1.0 4,163,141 + btuB b3966 0.80 +0.8 4,163,141 + btuB b3966 0.80 -0.1 4,163,146 + btuB b3966 0.80 -0.9 4,163,148 + btuB b3966 0.81 -1.4 4,163,156 - btuB b3966 0.81 -0.5 4,163,168 - btuB b3966 0.82 -2.2 4,163,212 - btuB b3966 0.84 -3.1 4,163,212 - btuB b3966 0.84 -2.8 4,163,214 + btuB b3966 0.84 -2.4 4,163,214 + btuB b3966 0.84 -0.7 4,163,269 + btuB b3966 0.87 -1.2 4,163,269 + btuB b3966 0.87 +0.1 4,163,272 - btuB b3966 0.87 +0.5 4,163,272 - btuB b3966 0.87 -2.1 4,163,317 + btuB b3966 0.90 -1.2 4,163,357 - -1.8 4,163,357 - -1.7 4,163,357 - -1.7 4,163,388 + +0.5 4,163,388 + -1.0 4,163,447 + -1.2 4,163,447 + -0.9 4,163,455 - -1.0 4,163,455 - -1.4 4,163,455 - -1.2 4,163,455 - -2.4 4,163,455 - -2.8 4,163,505 + +0.1
Or see this region's nucleotide sequence