Strain Fitness in Escherichia coli BW25113 around b1849

Experiment: D-Serine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebF and yebG are separated by 66 nucleotidesyebG and purT are separated by 133 nucleotidespurT and eda are separated by 55 nucleotideseda and edd are separated by 36 nucleotides b1847: yebF - predicted protein (RefSeq), at 1,928,058 to 1,928,414 yebF b1848: yebG - conserved protein regulated by LexA (NCBI), at 1,928,481 to 1,928,771 yebG b1849: purT - phosphoribosylglycinamide formyltransferase 2 (NCBI), at 1,928,905 to 1,930,083 purT b1850: eda - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI), at 1,930,139 to 1,930,780 eda b1851: edd - phosphogluconate dehydratase (NCBI), at 1,930,817 to 1,932,628 edd Position (kb) 1928 1929 1930 1931Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1928.014 kb on - strandat 1928.054 kb on + strandat 1928.054 kb on + strandat 1928.324 kb on + strand, within yebFat 1928.324 kb on + strand, within yebFat 1928.426 kb on + strandat 1928.426 kb on + strandat 1928.449 kb on - strandat 1928.449 kb on - strandat 1928.740 kb on - strand, within yebGat 1928.981 kb on - strandat 1928.981 kb on - strandat 1928.981 kb on - strandat 1929.008 kb on + strandat 1929.150 kb on - strand, within purTat 1929.362 kb on + strand, within purTat 1929.362 kb on + strand, within purTat 1929.640 kb on - strand, within purTat 1929.640 kb on - strand, within purTat 1929.683 kb on + strand, within purTat 1929.683 kb on + strand, within purTat 1929.759 kb on - strand, within purTat 1929.800 kb on + strand, within purTat 1930.003 kb on + strandat 1930.263 kb on + strand, within edaat 1930.411 kb on - strand, within edaat 1930.626 kb on - strand, within edaat 1930.626 kb on - strand, within edaat 1930.833 kb on + strandat 1930.841 kb on - strandat 1930.843 kb on + strandat 1930.843 kb on + strandat 1930.988 kb on + strandat 1931.044 kb on - strand, within eddat 1931.065 kb on - strand, within eddat 1931.065 kb on - strand, within edd

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (C)
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1,928,014 - -1.0
1,928,054 + +1.7
1,928,054 + -3.0
1,928,324 + yebF b1847 0.75 +0.5
1,928,324 + yebF b1847 0.75 +0.8
1,928,426 + +0.3
1,928,426 + -0.2
1,928,449 - +0.4
1,928,449 - +0.5
1,928,740 - yebG b1848 0.89 -0.3
1,928,981 - -0.6
1,928,981 - -0.0
1,928,981 - +0.1
1,929,008 + -0.2
1,929,150 - purT b1849 0.21 -0.6
1,929,362 + purT b1849 0.39 -1.8
1,929,362 + purT b1849 0.39 +0.7
1,929,640 - purT b1849 0.62 +1.4
1,929,640 - purT b1849 0.62 -1.3
1,929,683 + purT b1849 0.66 +0.2
1,929,683 + purT b1849 0.66 +0.1
1,929,759 - purT b1849 0.72 -0.8
1,929,800 + purT b1849 0.76 +1.0
1,930,003 + +2.2
1,930,263 + eda b1850 0.19 -1.1
1,930,411 - eda b1850 0.42 +0.1
1,930,626 - eda b1850 0.76 -0.1
1,930,626 - eda b1850 0.76 -0.5
1,930,833 + -0.7
1,930,841 - -0.4
1,930,843 + +0.8
1,930,843 + +0.9
1,930,988 + -0.1
1,931,044 - edd b1851 0.13 +0.5
1,931,065 - edd b1851 0.14 +1.1
1,931,065 - edd b1851 0.14 -0.9

Or see this region's nucleotide sequence