Experiment: D-Serine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entA and ybdB are separated by 2 nucleotides ybdB and cstA are separated by 180 nucleotides cstA and ybdD are separated by 182 nucleotides ybdD and ybdH are separated by 9 nucleotides
b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520
entA
b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936
ybdB
b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222
cstA
b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602
ybdD
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
Position (kb) 629
630
631
632 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 628.176 kb on + strand, within entA at 628.200 kb on + strand, within entA at 628.208 kb on - strand, within entA at 628.219 kb on - strand, within entA at 628.257 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.297 kb on + strand, within entA at 628.297 kb on + strand, within entA at 628.319 kb on - strand, within entA at 628.387 kb on - strand, within entA at 628.463 kb on - strand at 628.463 kb on - strand at 628.601 kb on + strand, within ybdB at 628.609 kb on - strand, within ybdB at 628.618 kb on - strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.790 kb on - strand, within ybdB at 628.923 kb on - strand at 628.923 kb on - strand at 629.097 kb on - strand at 629.097 kb on - strand at 629.099 kb on + strand at 629.100 kb on - strand at 629.140 kb on + strand at 629.140 kb on + strand at 629.140 kb on + strand at 629.182 kb on + strand at 629.189 kb on + strand at 629.189 kb on + strand at 629.275 kb on + strand at 629.275 kb on + strand at 629.330 kb on - strand, within cstA at 629.330 kb on - strand, within cstA at 629.333 kb on + strand, within cstA at 629.333 kb on + strand, within cstA at 629.435 kb on - strand, within cstA at 629.512 kb on - strand, within cstA at 629.630 kb on + strand, within cstA at 629.736 kb on - strand, within cstA at 629.736 kb on - strand, within cstA at 629.779 kb on + strand, within cstA at 629.797 kb on - strand, within cstA at 629.797 kb on - strand, within cstA at 629.798 kb on - strand, within cstA at 629.798 kb on - strand, within cstA at 629.798 kb on - strand, within cstA at 629.859 kb on + strand, within cstA at 629.859 kb on + strand, within cstA at 629.995 kb on - strand, within cstA at 629.995 kb on - strand, within cstA at 629.995 kb on - strand, within cstA at 629.995 kb on - strand, within cstA at 630.031 kb on + strand, within cstA at 630.036 kb on + strand, within cstA at 630.036 kb on + strand, within cstA at 630.036 kb on + strand, within cstA at 630.036 kb on + strand, within cstA at 630.071 kb on - strand, within cstA at 630.074 kb on + strand, within cstA at 630.074 kb on + strand, within cstA at 630.074 kb on + strand, within cstA at 630.089 kb on - strand, within cstA at 630.089 kb on - strand, within cstA at 630.153 kb on + strand, within cstA at 630.153 kb on + strand, within cstA at 630.164 kb on + strand, within cstA at 630.206 kb on - strand, within cstA at 630.259 kb on + strand, within cstA at 630.485 kb on + strand, within cstA at 630.486 kb on - strand, within cstA at 630.493 kb on - strand, within cstA at 630.511 kb on + strand, within cstA at 630.583 kb on + strand, within cstA at 630.640 kb on + strand, within cstA at 630.703 kb on + strand, within cstA at 630.766 kb on + strand, within cstA at 630.816 kb on + strand, within cstA at 630.834 kb on - strand, within cstA at 630.834 kb on - strand, within cstA at 630.853 kb on - strand, within cstA at 630.865 kb on + strand, within cstA at 630.865 kb on + strand, within cstA at 630.894 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 631.000 kb on + strand, within cstA at 631.000 kb on + strand, within cstA at 631.003 kb on + strand, within cstA at 631.068 kb on + strand at 631.068 kb on + strand at 631.126 kb on + strand at 631.180 kb on - strand at 631.194 kb on + strand at 631.194 kb on + strand at 631.236 kb on - strand at 631.250 kb on + strand at 631.262 kb on + strand at 631.262 kb on + strand at 631.264 kb on + strand at 631.278 kb on - strand at 631.300 kb on - strand at 631.300 kb on - strand at 631.305 kb on + strand at 631.398 kb on + strand at 631.398 kb on + strand at 631.401 kb on - strand at 631.413 kb on - strand at 631.413 kb on - strand at 631.780 kb on - strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH
Per-strain Table
Position Strand Gene LocusTag Fraction D-Serine (C) remove 628,176 + entA b0596 0.54 +0.1 628,200 + entA b0596 0.57 +2.4 628,208 - entA b0596 0.58 -0.3 628,219 - entA b0596 0.60 +0.2 628,257 - entA b0596 0.65 +0.8 628,287 - entA b0596 0.69 +0.5 628,287 - entA b0596 0.69 +1.4 628,297 + entA b0596 0.70 -0.9 628,297 + entA b0596 0.70 +1.8 628,319 - entA b0596 0.73 -0.4 628,387 - entA b0596 0.82 -1.0 628,463 - +0.1 628,463 - +1.7 628,601 + ybdB b0597 0.19 -1.1 628,609 - ybdB b0597 0.21 -0.1 628,618 - ybdB b0597 0.23 +0.3 628,640 + ybdB b0597 0.28 +0.0 628,640 + ybdB b0597 0.28 -1.2 628,756 + ybdB b0597 0.56 +0.5 628,756 + ybdB b0597 0.56 -1.0 628,790 - ybdB b0597 0.64 -1.0 628,923 - -0.0 628,923 - -0.9 629,097 - -1.8 629,097 - -0.1 629,099 + -0.3 629,100 - +0.3 629,140 + -0.1 629,140 + +0.1 629,140 + +0.1 629,182 + -1.4 629,189 + +0.9 629,189 + +2.9 629,275 + -1.0 629,275 + -0.9 629,330 - cstA b0598 0.10 -1.1 629,330 - cstA b0598 0.10 +0.9 629,333 + cstA b0598 0.10 -1.8 629,333 + cstA b0598 0.10 -0.8 629,435 - cstA b0598 0.15 -2.1 629,512 - cstA b0598 0.19 -0.8 629,630 + cstA b0598 0.24 -1.6 629,736 - cstA b0598 0.29 -1.6 629,736 - cstA b0598 0.29 -2.8 629,779 + cstA b0598 0.31 -2.1 629,797 - cstA b0598 0.32 -0.9 629,797 - cstA b0598 0.32 -1.6 629,798 - cstA b0598 0.32 -0.4 629,798 - cstA b0598 0.32 -1.7 629,798 - cstA b0598 0.32 -0.3 629,859 + cstA b0598 0.35 +0.9 629,859 + cstA b0598 0.35 +0.1 629,995 - cstA b0598 0.42 -2.1 629,995 - cstA b0598 0.42 -1.3 629,995 - cstA b0598 0.42 -0.5 629,995 - cstA b0598 0.42 -1.0 630,031 + cstA b0598 0.43 -2.8 630,036 + cstA b0598 0.44 -0.9 630,036 + cstA b0598 0.44 -1.4 630,036 + cstA b0598 0.44 -1.1 630,036 + cstA b0598 0.44 -0.6 630,071 - cstA b0598 0.45 -0.7 630,074 + cstA b0598 0.45 -0.4 630,074 + cstA b0598 0.45 -2.5 630,074 + cstA b0598 0.45 -0.1 630,089 - cstA b0598 0.46 -2.9 630,089 - cstA b0598 0.46 -0.3 630,153 + cstA b0598 0.49 -1.2 630,153 + cstA b0598 0.49 -1.4 630,164 + cstA b0598 0.50 -1.1 630,206 - cstA b0598 0.52 -1.6 630,259 + cstA b0598 0.54 -0.9 630,485 + cstA b0598 0.65 -0.3 630,486 - cstA b0598 0.65 -0.5 630,493 - cstA b0598 0.65 -1.6 630,511 + cstA b0598 0.66 -0.1 630,583 + cstA b0598 0.70 -0.4 630,640 + cstA b0598 0.72 +0.0 630,703 + cstA b0598 0.75 -0.3 630,766 + cstA b0598 0.78 -0.7 630,816 + cstA b0598 0.81 -1.5 630,834 - cstA b0598 0.82 -1.5 630,834 - cstA b0598 0.82 -0.8 630,853 - cstA b0598 0.82 -1.0 630,865 + cstA b0598 0.83 -1.0 630,865 + cstA b0598 0.83 +0.3 630,894 - cstA b0598 0.84 -0.6 630,927 - cstA b0598 0.86 -0.2 630,927 - cstA b0598 0.86 -1.3 631,000 + cstA b0598 0.89 -2.1 631,000 + cstA b0598 0.89 -0.3 631,003 + cstA b0598 0.90 -0.2 631,068 + -0.6 631,068 + +0.5 631,126 + -1.3 631,180 - -0.3 631,194 + -0.3 631,194 + -0.5 631,236 - -0.1 631,250 + +0.6 631,262 + -0.5 631,262 + -0.1 631,264 + -0.2 631,278 - +2.9 631,300 - -0.3 631,300 - -0.4 631,305 + -0.7 631,398 + -1.9 631,398 + +1.8 631,401 - -0.2 631,413 - -0.5 631,413 - -0.7 631,780 - ybdH b0599 0.15 +0.5 631,782 + ybdH b0599 0.16 +0.2 631,782 + ybdH b0599 0.16 +0.9 631,825 + ybdH b0599 0.20 +0.4 631,828 - ybdH b0599 0.20 +0.4 631,828 - ybdH b0599 0.20 +0.9 631,833 - ybdH b0599 0.20 -0.0 631,833 - ybdH b0599 0.20 -0.4 631,926 + ybdH b0599 0.29 -0.0 631,926 - ybdH b0599 0.29 -0.9 632,035 + ybdH b0599 0.39 -0.9 632,048 + ybdH b0599 0.40 +0.3 632,147 + ybdH b0599 0.49 +0.6 632,160 + ybdH b0599 0.50 +0.0
Or see this region's nucleotide sequence