Strain Fitness in Escherichia coli BW25113 around b0383

Experiment: D-Serine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntddlA and yaiB are separated by 462 nucleotidesyaiB and phoA are separated by 100 nucleotidesphoA and psiF are separated by 118 nucleotidespsiF and yaiC are separated by 101 nucleotides b0381: ddlA - D-alanylalanine synthetase (NCBI), at 399,053 to 400,147 ddlA b0382: yaiB - hypothetical protein (NCBI), at 400,610 to 400,870 yaiB b0383: phoA - alkaline phosphatase (VIMSS), at 400,971 to 402,386 phoA b0384: psiF - induced by phosphate starvation (VIMSS), at 402,505 to 402,825 psiF b0385: yaiC - predicted diguanylate cyclase (NCBI), at 402,927 to 404,042 yaiC Position (kb) 400 401 402 403Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 400.092 kb on + strandat 400.092 kb on + strandat 400.093 kb on + strandat 400.093 kb on + strandat 400.168 kb on + strandat 400.178 kb on - strandat 400.240 kb on + strandat 400.240 kb on + strandat 400.251 kb on + strandat 400.258 kb on + strandat 400.263 kb on + strandat 400.264 kb on + strandat 400.282 kb on - strandat 400.288 kb on - strandat 400.373 kb on + strandat 400.559 kb on - strandat 400.561 kb on + strandat 400.638 kb on - strand, within yaiBat 400.667 kb on + strand, within yaiBat 400.675 kb on + strand, within yaiBat 400.684 kb on + strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.692 kb on - strand, within yaiBat 400.707 kb on + strand, within yaiBat 400.715 kb on - strand, within yaiBat 400.750 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.762 kb on + strand, within yaiBat 400.770 kb on - strand, within yaiBat 400.770 kb on - strand, within yaiBat 400.775 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.777 kb on + strand, within yaiBat 400.782 kb on + strand, within yaiBat 400.783 kb on + strand, within yaiBat 400.783 kb on + strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.783 kb on - strand, within yaiBat 400.812 kb on + strand, within yaiBat 400.812 kb on - strand, within yaiBat 400.815 kb on + strand, within yaiBat 400.815 kb on + strand, within yaiBat 400.816 kb on - strand, within yaiBat 400.823 kb on - strand, within yaiBat 400.828 kb on - strand, within yaiBat 400.835 kb on + strand, within yaiBat 400.865 kb on - strandat 400.898 kb on - strandat 400.899 kb on + strandat 400.901 kb on - strandat 400.901 kb on - strandat 400.915 kb on + strandat 400.915 kb on + strandat 400.982 kb on - strandat 400.992 kb on - strandat 400.998 kb on - strandat 401.004 kb on + strandat 401.006 kb on - strandat 401.006 kb on - strandat 401.015 kb on - strandat 401.015 kb on - strandat 401.020 kb on - strandat 401.078 kb on - strandat 401.086 kb on - strandat 401.088 kb on - strandat 401.088 kb on - strandat 401.091 kb on - strandat 401.091 kb on - strandat 401.105 kb on + strandat 401.113 kb on - strand, within phoAat 401.113 kb on - strand, within phoAat 401.113 kb on - strand, within phoAat 401.120 kb on + strand, within phoAat 401.123 kb on + strand, within phoAat 401.123 kb on + strand, within phoAat 401.125 kb on + strand, within phoAat 401.126 kb on - strand, within phoAat 401.133 kb on - strand, within phoAat 401.192 kb on - strand, within phoAat 401.199 kb on - strand, within phoAat 401.217 kb on - strand, within phoAat 401.271 kb on - strand, within phoAat 401.280 kb on + strand, within phoAat 401.282 kb on + strand, within phoAat 401.290 kb on - strand, within phoAat 401.332 kb on - strand, within phoAat 401.348 kb on + strand, within phoAat 401.348 kb on + strand, within phoAat 401.350 kb on - strand, within phoAat 401.350 kb on - strand, within phoAat 401.350 kb on - strand, within phoAat 401.363 kb on + strand, within phoAat 401.380 kb on + strand, within phoAat 401.486 kb on + strand, within phoAat 401.486 kb on + strand, within phoAat 401.494 kb on - strand, within phoAat 401.503 kb on + strand, within phoAat 401.616 kb on + strand, within phoAat 401.621 kb on + strand, within phoAat 401.653 kb on - strand, within phoAat 401.800 kb on + strand, within phoAat 401.800 kb on + strand, within phoAat 401.808 kb on - strand, within phoAat 401.808 kb on - strand, within phoAat 401.830 kb on - strand, within phoAat 401.839 kb on + strand, within phoAat 401.888 kb on + strand, within phoAat 401.930 kb on - strand, within phoAat 402.081 kb on + strand, within phoAat 402.081 kb on + strand, within phoAat 402.086 kb on - strand, within phoAat 402.144 kb on - strand, within phoAat 402.183 kb on + strand, within phoAat 402.218 kb on - strand, within phoAat 402.218 kb on - strand, within phoAat 402.246 kb on - strandat 402.291 kb on + strandat 402.291 kb on + strandat 402.350 kb on + strandat 402.605 kb on - strand, within psiFat 402.605 kb on - strand, within psiFat 402.689 kb on - strand, within psiFat 402.689 kb on - strand, within psiFat 402.733 kb on - strand, within psiFat 402.733 kb on - strand, within psiFat 402.826 kb on - strandat 402.827 kb on + strandat 402.835 kb on - strandat 402.840 kb on + strandat 402.873 kb on - strandat 402.873 kb on - strandat 403.026 kb on + strandat 403.050 kb on + strand, within yaiCat 403.050 kb on + strand, within yaiCat 403.058 kb on - strand, within yaiCat 403.063 kb on + strand, within yaiCat 403.130 kb on + strand, within yaiCat 403.291 kb on + strand, within yaiC

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (C)
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400,092 + -0.0
400,092 + +1.0
400,093 + +1.4
400,093 + +0.8
400,168 + +0.4
400,178 - +0.1
400,240 + +0.6
400,240 + +1.2
400,251 + +0.5
400,258 + +0.4
400,263 + -0.4
400,264 + -0.1
400,282 - +0.2
400,288 - -1.0
400,373 + -0.4
400,559 - +4.9
400,561 + +0.2
400,638 - yaiB b0382 0.11 +0.6
400,667 + yaiB b0382 0.22 -0.1
400,675 + yaiB b0382 0.25 +1.3
400,684 + yaiB b0382 0.28 +0.8
400,692 - yaiB b0382 0.31 +0.2
400,692 - yaiB b0382 0.31 +1.0
400,692 - yaiB b0382 0.31 +0.7
400,707 + yaiB b0382 0.37 +1.4
400,715 - yaiB b0382 0.40 +0.7
400,750 + yaiB b0382 0.54 +0.9
400,762 + yaiB b0382 0.58 +0.1
400,762 + yaiB b0382 0.58 +0.7
400,762 + yaiB b0382 0.58 +0.5
400,770 - yaiB b0382 0.61 +0.2
400,770 - yaiB b0382 0.61 +0.9
400,775 + yaiB b0382 0.63 +0.7
400,777 + yaiB b0382 0.64 -0.2
400,777 + yaiB b0382 0.64 +1.0
400,777 + yaiB b0382 0.64 +0.5
400,777 + yaiB b0382 0.64 +1.3
400,782 + yaiB b0382 0.66 +0.3
400,783 + yaiB b0382 0.66 +0.2
400,783 + yaiB b0382 0.66 +0.2
400,783 - yaiB b0382 0.66 +1.3
400,783 - yaiB b0382 0.66 +0.7
400,783 - yaiB b0382 0.66 -0.3
400,812 + yaiB b0382 0.77 +0.3
400,812 - yaiB b0382 0.77 +0.3
400,815 + yaiB b0382 0.79 -0.8
400,815 + yaiB b0382 0.79 +0.4
400,816 - yaiB b0382 0.79 +0.4
400,823 - yaiB b0382 0.82 +1.4
400,828 - yaiB b0382 0.84 +0.7
400,835 + yaiB b0382 0.86 +0.5
400,865 - +1.2
400,898 - +0.9
400,899 + -0.2
400,901 - +1.0
400,901 - +0.7
400,915 + -0.2
400,915 + +0.0
400,982 - +0.1
400,992 - +1.6
400,998 - +0.1
401,004 + +0.5
401,006 - -0.5
401,006 - +0.5
401,015 - +1.4
401,015 - +0.9
401,020 - +0.4
401,078 - -0.1
401,086 - +0.2
401,088 - -0.2
401,088 - +1.0
401,091 - +0.7
401,091 - +0.3
401,105 + +0.6
401,113 - phoA b0383 0.10 +0.4
401,113 - phoA b0383 0.10 +0.5
401,113 - phoA b0383 0.10 -0.0
401,120 + phoA b0383 0.11 -0.1
401,123 + phoA b0383 0.11 -0.3
401,123 + phoA b0383 0.11 -0.1
401,125 + phoA b0383 0.11 +0.1
401,126 - phoA b0383 0.11 +0.3
401,133 - phoA b0383 0.11 -1.4
401,192 - phoA b0383 0.16 +0.6
401,199 - phoA b0383 0.16 -0.0
401,217 - phoA b0383 0.17 -0.4
401,271 - phoA b0383 0.21 +0.4
401,280 + phoA b0383 0.22 -0.3
401,282 + phoA b0383 0.22 +0.1
401,290 - phoA b0383 0.23 -0.1
401,332 - phoA b0383 0.25 +0.5
401,348 + phoA b0383 0.27 -0.3
401,348 + phoA b0383 0.27 -0.3
401,350 - phoA b0383 0.27 -0.7
401,350 - phoA b0383 0.27 -0.5
401,350 - phoA b0383 0.27 +1.4
401,363 + phoA b0383 0.28 -0.2
401,380 + phoA b0383 0.29 -0.6
401,486 + phoA b0383 0.36 +0.7
401,486 + phoA b0383 0.36 -0.2
401,494 - phoA b0383 0.37 -0.1
401,503 + phoA b0383 0.38 -0.2
401,616 + phoA b0383 0.46 +0.1
401,621 + phoA b0383 0.46 -0.8
401,653 - phoA b0383 0.48 +0.6
401,800 + phoA b0383 0.59 +0.6
401,800 + phoA b0383 0.59 +0.2
401,808 - phoA b0383 0.59 +0.7
401,808 - phoA b0383 0.59 +0.6
401,830 - phoA b0383 0.61 +2.4
401,839 + phoA b0383 0.61 -0.1
401,888 + phoA b0383 0.65 -0.2
401,930 - phoA b0383 0.68 -0.9
402,081 + phoA b0383 0.78 +0.6
402,081 + phoA b0383 0.78 +0.2
402,086 - phoA b0383 0.79 +0.0
402,144 - phoA b0383 0.83 -0.5
402,183 + phoA b0383 0.86 +0.7
402,218 - phoA b0383 0.88 +0.6
402,218 - phoA b0383 0.88 +1.2
402,246 - +0.0
402,291 + +0.6
402,291 + -1.0
402,350 + -0.3
402,605 - psiF b0384 0.31 -0.6
402,605 - psiF b0384 0.31 -0.5
402,689 - psiF b0384 0.57 +0.6
402,689 - psiF b0384 0.57 +0.5
402,733 - psiF b0384 0.71 -0.8
402,733 - psiF b0384 0.71 -0.8
402,826 - +0.1
402,827 + +0.3
402,835 - -0.3
402,840 + -0.5
402,873 - -0.1
402,873 - +0.1
403,026 + -0.5
403,050 + yaiC b0385 0.11 -1.0
403,050 + yaiC b0385 0.11 -0.8
403,058 - yaiC b0385 0.12 +0.1
403,063 + yaiC b0385 0.12 +0.7
403,130 + yaiC b0385 0.18 -0.3
403,291 + yaiC b0385 0.33 +0.3

Or see this region's nucleotide sequence