Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4399 and EX28DRAFT_4400 are separated by 21 nucleotides EX28DRAFT_4400 and EX28DRAFT_4401 are separated by 399 nucleotides EX28DRAFT_4401 and EX28DRAFT_4402 are separated by 83 nucleotides
EX28DRAFT_4399: EX28DRAFT_4399 - glycerol kinase, at 61,538 to 63,046
_4399
EX28DRAFT_4400: EX28DRAFT_4400 - MIP family channel proteins, at 63,068 to 63,913
_4400
EX28DRAFT_4401: EX28DRAFT_4401 - Uncharacterized protein conserved in bacteria, at 64,313 to 64,552
_4401
EX28DRAFT_4402: EX28DRAFT_4402 - regulator of ribonuclease activity A, at 64,636 to 65,121
_4402
Position (kb)
63
64 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 62.145 kb on + strand, within EX28DRAFT_4399 at 62.233 kb on + strand, within EX28DRAFT_4399 at 62.233 kb on + strand, within EX28DRAFT_4399 at 62.250 kb on + strand, within EX28DRAFT_4399 at 62.250 kb on + strand, within EX28DRAFT_4399 at 62.250 kb on + strand, within EX28DRAFT_4399 at 62.251 kb on - strand, within EX28DRAFT_4399 at 62.363 kb on - strand, within EX28DRAFT_4399 at 62.497 kb on - strand, within EX28DRAFT_4399 at 62.578 kb on + strand, within EX28DRAFT_4399 at 62.600 kb on + strand, within EX28DRAFT_4399 at 62.640 kb on + strand, within EX28DRAFT_4399 at 62.677 kb on - strand, within EX28DRAFT_4399 at 62.677 kb on - strand, within EX28DRAFT_4399 at 62.751 kb on + strand, within EX28DRAFT_4399 at 62.752 kb on - strand, within EX28DRAFT_4399 at 62.755 kb on + strand, within EX28DRAFT_4399 at 62.755 kb on + strand, within EX28DRAFT_4399 at 63.049 kb on + strand at 63.049 kb on + strand at 63.049 kb on + strand at 63.049 kb on + strand at 63.050 kb on - strand at 63.050 kb on - strand at 63.050 kb on - strand at 63.138 kb on - strand at 63.152 kb on + strand at 63.153 kb on - strand, within EX28DRAFT_4400 at 63.166 kb on + strand, within EX28DRAFT_4400 at 63.166 kb on + strand, within EX28DRAFT_4400 at 63.166 kb on + strand, within EX28DRAFT_4400 at 63.167 kb on - strand, within EX28DRAFT_4400 at 63.219 kb on + strand, within EX28DRAFT_4400 at 63.553 kb on + strand, within EX28DRAFT_4400 at 63.553 kb on + strand, within EX28DRAFT_4400 at 63.587 kb on - strand, within EX28DRAFT_4400 at 63.588 kb on + strand, within EX28DRAFT_4400 at 63.588 kb on + strand, within EX28DRAFT_4400 at 63.589 kb on - strand, within EX28DRAFT_4400 at 63.591 kb on + strand, within EX28DRAFT_4400 at 63.592 kb on - strand, within EX28DRAFT_4400 at 63.600 kb on + strand, within EX28DRAFT_4400 at 63.656 kb on + strand, within EX28DRAFT_4400 at 63.657 kb on - strand, within EX28DRAFT_4400 at 63.657 kb on - strand, within EX28DRAFT_4400 at 63.744 kb on + strand, within EX28DRAFT_4400 at 63.744 kb on + strand, within EX28DRAFT_4400 at 63.744 kb on + strand, within EX28DRAFT_4400 at 63.745 kb on - strand, within EX28DRAFT_4400 at 63.778 kb on + strand, within EX28DRAFT_4400 at 63.893 kb on + strand at 63.948 kb on - strand at 63.948 kb on - strand at 63.988 kb on - strand at 63.988 kb on - strand at 64.152 kb on + strand at 64.152 kb on + strand at 64.153 kb on - strand at 64.153 kb on - strand at 64.154 kb on + strand at 64.155 kb on - strand at 64.155 kb on - strand at 64.243 kb on + strand at 64.243 kb on + strand at 64.244 kb on - strand at 64.244 kb on - strand at 64.244 kb on - strand at 64.255 kb on + strand at 64.256 kb on - strand at 64.257 kb on + strand at 64.257 kb on + strand at 64.257 kb on + strand at 64.257 kb on + strand at 64.258 kb on - strand at 64.258 kb on - strand at 64.273 kb on - strand at 64.273 kb on - strand at 64.306 kb on + strand at 64.306 kb on + strand at 64.307 kb on - strand at 64.307 kb on - strand at 64.320 kb on + strand at 64.321 kb on - strand at 64.537 kb on + strand at 64.567 kb on + strand at 64.567 kb on + strand at 64.578 kb on + strand at 64.578 kb on + strand at 64.579 kb on - strand at 64.637 kb on + strand at 64.638 kb on - strand at 64.688 kb on + strand, within EX28DRAFT_4402 at 64.688 kb on + strand, within EX28DRAFT_4402 at 64.689 kb on - strand, within EX28DRAFT_4402 at 64.903 kb on + strand, within EX28DRAFT_4402 at 64.903 kb on + strand, within EX28DRAFT_4402 at 64.904 kb on - strand, within EX28DRAFT_4402
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 62,145 + EX28DRAFT_4399 0.40 -1.0 62,233 + EX28DRAFT_4399 0.46 -1.0 62,233 + EX28DRAFT_4399 0.46 -0.5 62,250 + EX28DRAFT_4399 0.47 +1.0 62,250 + EX28DRAFT_4399 0.47 -0.8 62,250 + EX28DRAFT_4399 0.47 +0.2 62,251 - EX28DRAFT_4399 0.47 -1.5 62,363 - EX28DRAFT_4399 0.55 -2.0 62,497 - EX28DRAFT_4399 0.64 -0.3 62,578 + EX28DRAFT_4399 0.69 -1.2 62,600 + EX28DRAFT_4399 0.70 +0.9 62,640 + EX28DRAFT_4399 0.73 -0.0 62,677 - EX28DRAFT_4399 0.75 +0.4 62,677 - EX28DRAFT_4399 0.75 +1.1 62,751 + EX28DRAFT_4399 0.80 +0.1 62,752 - EX28DRAFT_4399 0.80 -0.5 62,755 + EX28DRAFT_4399 0.81 -0.8 62,755 + EX28DRAFT_4399 0.81 -1.0 63,049 + +0.2 63,049 + -0.7 63,049 + +0.1 63,049 + -0.3 63,050 - -0.2 63,050 - -0.5 63,050 - +0.1 63,138 - -0.6 63,152 + +1.4 63,153 - EX28DRAFT_4400 0.10 -0.1 63,166 + EX28DRAFT_4400 0.12 +0.6 63,166 + EX28DRAFT_4400 0.12 +0.7 63,166 + EX28DRAFT_4400 0.12 +0.3 63,167 - EX28DRAFT_4400 0.12 -0.6 63,219 + EX28DRAFT_4400 0.18 +0.2 63,553 + EX28DRAFT_4400 0.57 +2.1 63,553 + EX28DRAFT_4400 0.57 -0.0 63,587 - EX28DRAFT_4400 0.61 +0.3 63,588 + EX28DRAFT_4400 0.61 -0.0 63,588 + EX28DRAFT_4400 0.61 +0.1 63,589 - EX28DRAFT_4400 0.62 -0.1 63,591 + EX28DRAFT_4400 0.62 -0.7 63,592 - EX28DRAFT_4400 0.62 +0.3 63,600 + EX28DRAFT_4400 0.63 +0.9 63,656 + EX28DRAFT_4400 0.70 +3.3 63,657 - EX28DRAFT_4400 0.70 +0.9 63,657 - EX28DRAFT_4400 0.70 +0.6 63,744 + EX28DRAFT_4400 0.80 +0.8 63,744 + EX28DRAFT_4400 0.80 +0.5 63,744 + EX28DRAFT_4400 0.80 -0.2 63,745 - EX28DRAFT_4400 0.80 -0.3 63,778 + EX28DRAFT_4400 0.84 -0.2 63,893 + -0.3 63,948 - +0.2 63,948 - +0.9 63,988 - -0.1 63,988 - +1.0 64,152 + -0.1 64,152 + +0.1 64,153 - -1.2 64,153 - +2.9 64,154 + +0.6 64,155 - +0.0 64,155 - +0.1 64,243 + +1.4 64,243 + +0.6 64,244 - -0.0 64,244 - -0.5 64,244 - +0.5 64,255 + +0.5 64,256 - +0.2 64,257 + +2.3 64,257 + +1.0 64,257 + +0.3 64,257 + -0.9 64,258 - +0.9 64,258 - -0.1 64,273 - -0.4 64,273 - +0.9 64,306 + +0.7 64,306 + -0.5 64,307 - -0.3 64,307 - +1.8 64,320 + +0.3 64,321 - +0.2 64,537 + +0.1 64,567 + -0.4 64,567 + -0.0 64,578 + +0.4 64,578 + +0.5 64,579 - -0.1 64,637 + -0.3 64,638 - -0.8 64,688 + EX28DRAFT_4402 0.11 -0.3 64,688 + EX28DRAFT_4402 0.11 +0.5 64,689 - EX28DRAFT_4402 0.11 +0.3 64,903 + EX28DRAFT_4402 0.55 +0.1 64,903 + EX28DRAFT_4402 0.55 +1.0 64,904 - EX28DRAFT_4402 0.55 +0.2
Or see this region's nucleotide sequence