Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4027 and EX28DRAFT_4028 are separated by 106 nucleotides EX28DRAFT_4028 and EX28DRAFT_4029 are separated by 12 nucleotides EX28DRAFT_4029 and EX28DRAFT_4030 overlap by 35 nucleotides
EX28DRAFT_4027: EX28DRAFT_4027 - ABC-type sugar transport system, periplasmic component, at 238,982 to 239,872
_4027
EX28DRAFT_4028: EX28DRAFT_4028 - ribokinase, at 239,979 to 240,908
_4028
EX28DRAFT_4029: EX28DRAFT_4029 - transcriptional regulator, LacI family, at 240,921 to 241,904
_4029
EX28DRAFT_4030: EX28DRAFT_4030 - drug resistance transporter, EmrB/QacA subfamily, at 241,870 to 243,303
_4030
Position (kb) 239
240
241 Strain fitness (log2 ratio) -2
-1
0
1
2 at 239.037 kb on + strand at 239.038 kb on - strand at 239.150 kb on + strand, within EX28DRAFT_4027 at 239.214 kb on + strand, within EX28DRAFT_4027 at 239.268 kb on + strand, within EX28DRAFT_4027 at 239.269 kb on - strand, within EX28DRAFT_4027 at 239.272 kb on + strand, within EX28DRAFT_4027 at 239.399 kb on + strand, within EX28DRAFT_4027 at 239.399 kb on + strand, within EX28DRAFT_4027 at 239.399 kb on + strand, within EX28DRAFT_4027 at 239.554 kb on + strand, within EX28DRAFT_4027 at 239.557 kb on + strand, within EX28DRAFT_4027 at 239.557 kb on + strand, within EX28DRAFT_4027 at 239.558 kb on - strand, within EX28DRAFT_4027 at 239.558 kb on - strand, within EX28DRAFT_4027 at 239.558 kb on - strand, within EX28DRAFT_4027 at 239.558 kb on - strand, within EX28DRAFT_4027 at 239.671 kb on + strand, within EX28DRAFT_4027 at 239.870 kb on + strand at 239.871 kb on - strand at 239.871 kb on - strand at 239.914 kb on + strand at 239.931 kb on + strand at 240.085 kb on - strand, within EX28DRAFT_4028 at 240.197 kb on - strand, within EX28DRAFT_4028 at 240.221 kb on + strand, within EX28DRAFT_4028 at 240.221 kb on + strand, within EX28DRAFT_4028 at 240.458 kb on + strand, within EX28DRAFT_4028 at 240.474 kb on - strand, within EX28DRAFT_4028 at 240.494 kb on - strand, within EX28DRAFT_4028 at 240.494 kb on - strand, within EX28DRAFT_4028 at 240.620 kb on + strand, within EX28DRAFT_4028 at 240.726 kb on - strand, within EX28DRAFT_4028 at 240.744 kb on - strand, within EX28DRAFT_4028 at 240.800 kb on + strand, within EX28DRAFT_4028 at 240.880 kb on + strand at 241.044 kb on + strand, within EX28DRAFT_4029 at 241.074 kb on - strand, within EX28DRAFT_4029 at 241.100 kb on + strand, within EX28DRAFT_4029 at 241.132 kb on - strand, within EX28DRAFT_4029 at 241.196 kb on + strand, within EX28DRAFT_4029 at 241.196 kb on + strand, within EX28DRAFT_4029 at 241.388 kb on + strand, within EX28DRAFT_4029 at 241.507 kb on - strand, within EX28DRAFT_4029 at 241.683 kb on + strand, within EX28DRAFT_4029 at 241.684 kb on - strand, within EX28DRAFT_4029 at 241.738 kb on + strand, within EX28DRAFT_4029 at 241.753 kb on - strand, within EX28DRAFT_4029 at 241.833 kb on - strand at 241.872 kb on - strand at 241.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 239,037 + -1.8 239,038 - +0.3 239,150 + EX28DRAFT_4027 0.19 +0.8 239,214 + EX28DRAFT_4027 0.26 +0.2 239,268 + EX28DRAFT_4027 0.32 +0.1 239,269 - EX28DRAFT_4027 0.32 +1.2 239,272 + EX28DRAFT_4027 0.33 -0.9 239,399 + EX28DRAFT_4027 0.47 +0.3 239,399 + EX28DRAFT_4027 0.47 -0.5 239,399 + EX28DRAFT_4027 0.47 +0.0 239,554 + EX28DRAFT_4027 0.64 -0.5 239,557 + EX28DRAFT_4027 0.65 -0.4 239,557 + EX28DRAFT_4027 0.65 -0.2 239,558 - EX28DRAFT_4027 0.65 -0.1 239,558 - EX28DRAFT_4027 0.65 -0.1 239,558 - EX28DRAFT_4027 0.65 -0.3 239,558 - EX28DRAFT_4027 0.65 -1.5 239,671 + EX28DRAFT_4027 0.77 +1.0 239,870 + +0.1 239,871 - +0.3 239,871 - +1.8 239,914 + +0.4 239,931 + +0.1 240,085 - EX28DRAFT_4028 0.11 +0.5 240,197 - EX28DRAFT_4028 0.23 +0.3 240,221 + EX28DRAFT_4028 0.26 +0.5 240,221 + EX28DRAFT_4028 0.26 +0.9 240,458 + EX28DRAFT_4028 0.52 -0.5 240,474 - EX28DRAFT_4028 0.53 +1.4 240,494 - EX28DRAFT_4028 0.55 +0.1 240,494 - EX28DRAFT_4028 0.55 +0.8 240,620 + EX28DRAFT_4028 0.69 -0.1 240,726 - EX28DRAFT_4028 0.80 -0.2 240,744 - EX28DRAFT_4028 0.82 -0.8 240,800 + EX28DRAFT_4028 0.88 +0.8 240,880 + -0.3 241,044 + EX28DRAFT_4029 0.12 -0.8 241,074 - EX28DRAFT_4029 0.16 -2.3 241,100 + EX28DRAFT_4029 0.18 -0.0 241,132 - EX28DRAFT_4029 0.21 -0.5 241,196 + EX28DRAFT_4029 0.28 +0.3 241,196 + EX28DRAFT_4029 0.28 +0.8 241,388 + EX28DRAFT_4029 0.47 -0.5 241,507 - EX28DRAFT_4029 0.60 +1.0 241,683 + EX28DRAFT_4029 0.77 +0.3 241,684 - EX28DRAFT_4029 0.78 +0.5 241,738 + EX28DRAFT_4029 0.83 +0.5 241,753 - EX28DRAFT_4029 0.85 +0.1 241,833 - +1.9 241,872 - +0.4 241,903 - +0.3
Or see this region's nucleotide sequence