Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3935 and EX28DRAFT_3936 are separated by 121 nucleotides EX28DRAFT_3936 and EX28DRAFT_3937 are separated by 44 nucleotides
EX28DRAFT_3935: EX28DRAFT_3935 - efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family, at 145,185 to 146,549
_3935
EX28DRAFT_3936: EX28DRAFT_3936 - hypothetical protein, at 146,671 to 146,967
_3936
EX28DRAFT_3937: EX28DRAFT_3937 - Transcriptional regulators, at 147,012 to 147,962
_3937
Position (kb) 146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 146.358 kb on + strand, within EX28DRAFT_3935 at 146.358 kb on + strand, within EX28DRAFT_3935 at 146.404 kb on - strand, within EX28DRAFT_3935 at 146.404 kb on - strand, within EX28DRAFT_3935 at 146.424 kb on + strand at 146.424 kb on + strand at 146.424 kb on + strand at 146.565 kb on - strand at 146.565 kb on - strand at 146.623 kb on + strand at 146.629 kb on + strand at 146.631 kb on + strand at 146.631 kb on + strand at 146.631 kb on + strand at 146.632 kb on - strand at 146.656 kb on + strand at 146.793 kb on + strand, within EX28DRAFT_3936 at 146.794 kb on - strand, within EX28DRAFT_3936 at 146.794 kb on - strand, within EX28DRAFT_3936 at 146.924 kb on - strand, within EX28DRAFT_3936 at 146.963 kb on - strand at 147.058 kb on + strand at 147.058 kb on + strand at 147.058 kb on + strand at 147.058 kb on + strand at 147.058 kb on + strand at 147.059 kb on - strand at 147.059 kb on - strand at 147.059 kb on - strand at 147.128 kb on - strand, within EX28DRAFT_3937 at 147.191 kb on + strand, within EX28DRAFT_3937 at 147.192 kb on - strand, within EX28DRAFT_3937 at 147.302 kb on + strand, within EX28DRAFT_3937 at 147.478 kb on + strand, within EX28DRAFT_3937 at 147.479 kb on - strand, within EX28DRAFT_3937 at 147.482 kb on + strand, within EX28DRAFT_3937 at 147.482 kb on + strand, within EX28DRAFT_3937 at 147.483 kb on - strand, within EX28DRAFT_3937 at 147.483 kb on - strand, within EX28DRAFT_3937 at 147.528 kb on - strand, within EX28DRAFT_3937 at 147.533 kb on + strand, within EX28DRAFT_3937 at 147.879 kb on + strand at 147.942 kb on + strand at 147.942 kb on + strand at 147.943 kb on - strand at 147.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 146,358 + EX28DRAFT_3935 0.86 -0.6 146,358 + EX28DRAFT_3935 0.86 -0.7 146,404 - EX28DRAFT_3935 0.89 -0.2 146,404 - EX28DRAFT_3935 0.89 -0.5 146,424 + +0.0 146,424 + +0.0 146,424 + +0.2 146,565 - +0.5 146,565 - +2.2 146,623 + -0.7 146,629 + -1.1 146,631 + -0.0 146,631 + +0.8 146,631 + -0.7 146,632 - -0.0 146,656 + -1.0 146,793 + EX28DRAFT_3936 0.41 +1.0 146,794 - EX28DRAFT_3936 0.41 -0.5 146,794 - EX28DRAFT_3936 0.41 +1.0 146,924 - EX28DRAFT_3936 0.85 -2.1 146,963 - +0.3 147,058 + +0.2 147,058 + -1.4 147,058 + +0.7 147,058 + -0.3 147,058 + -0.7 147,059 - +0.6 147,059 - +0.2 147,059 - +0.8 147,128 - EX28DRAFT_3937 0.12 -0.0 147,191 + EX28DRAFT_3937 0.19 +0.6 147,192 - EX28DRAFT_3937 0.19 +1.2 147,302 + EX28DRAFT_3937 0.30 +0.0 147,478 + EX28DRAFT_3937 0.49 +0.1 147,479 - EX28DRAFT_3937 0.49 -0.3 147,482 + EX28DRAFT_3937 0.49 +0.6 147,482 + EX28DRAFT_3937 0.49 +0.0 147,483 - EX28DRAFT_3937 0.50 -0.3 147,483 - EX28DRAFT_3937 0.50 -0.3 147,528 - EX28DRAFT_3937 0.54 -0.9 147,533 + EX28DRAFT_3937 0.55 +0.1 147,879 + -0.2 147,942 + +0.2 147,942 + +0.5 147,943 - -0.2 147,943 - -1.0
Or see this region's nucleotide sequence