Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3891 and EX28DRAFT_3892 are separated by 40 nucleotides EX28DRAFT_3892 and EX28DRAFT_3893 are separated by 67 nucleotides EX28DRAFT_3893 and EX28DRAFT_3894 are separated by 125 nucleotides
EX28DRAFT_3891: EX28DRAFT_3891 - transcriptional regulator, TetR family, at 99,345 to 99,941
_3891
EX28DRAFT_3892: EX28DRAFT_3892 - orotate phosphoribosyltransferase (EC 2.4.2.10), at 99,982 to 100,623
_3892
EX28DRAFT_3893: EX28DRAFT_3893 - ribonuclease PH, at 100,691 to 101,407
_3893
EX28DRAFT_3894: EX28DRAFT_3894 - TIGR00255 family protein, at 101,533 to 102,396
_3894
Position (kb) 100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 99.738 kb on - strand, within EX28DRAFT_3891 at 99.870 kb on - strand, within EX28DRAFT_3891 at 99.882 kb on + strand at 99.940 kb on - strand at 99.946 kb on + strand at 99.979 kb on + strand at 99.984 kb on - strand at 100.841 kb on + strand, within EX28DRAFT_3893 at 100.842 kb on - strand, within EX28DRAFT_3893 at 100.913 kb on - strand, within EX28DRAFT_3893 at 101.150 kb on - strand, within EX28DRAFT_3893 at 101.303 kb on - strand, within EX28DRAFT_3893 at 101.349 kb on + strand at 101.446 kb on + strand at 101.448 kb on + strand at 101.465 kb on + strand at 101.466 kb on - strand at 101.494 kb on + strand at 101.495 kb on - strand at 101.497 kb on - strand at 101.532 kb on + strand at 101.544 kb on + strand at 101.577 kb on + strand at 101.678 kb on + strand, within EX28DRAFT_3894 at 101.679 kb on - strand, within EX28DRAFT_3894 at 101.694 kb on + strand, within EX28DRAFT_3894 at 101.694 kb on + strand, within EX28DRAFT_3894 at 101.695 kb on - strand, within EX28DRAFT_3894 at 101.724 kb on + strand, within EX28DRAFT_3894 at 101.724 kb on + strand, within EX28DRAFT_3894 at 101.725 kb on - strand, within EX28DRAFT_3894 at 101.856 kb on + strand, within EX28DRAFT_3894 at 101.856 kb on + strand, within EX28DRAFT_3894 at 101.861 kb on + strand, within EX28DRAFT_3894 at 101.862 kb on - strand, within EX28DRAFT_3894 at 102.052 kb on - strand, within EX28DRAFT_3894 at 102.062 kb on + strand, within EX28DRAFT_3894 at 102.062 kb on + strand, within EX28DRAFT_3894 at 102.165 kb on + strand, within EX28DRAFT_3894 at 102.166 kb on - strand, within EX28DRAFT_3894 at 102.166 kb on - strand, within EX28DRAFT_3894 at 102.217 kb on + strand, within EX28DRAFT_3894 at 102.218 kb on - strand, within EX28DRAFT_3894 at 102.264 kb on + strand, within EX28DRAFT_3894 at 102.264 kb on + strand, within EX28DRAFT_3894 at 102.264 kb on + strand, within EX28DRAFT_3894
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 99,738 - EX28DRAFT_3891 0.66 -1.2 99,870 - EX28DRAFT_3891 0.88 -0.3 99,882 + -0.0 99,940 - +3.4 99,946 + -1.5 99,979 + +0.2 99,984 - +0.7 100,841 + EX28DRAFT_3893 0.21 -2.1 100,842 - EX28DRAFT_3893 0.21 +1.0 100,913 - EX28DRAFT_3893 0.31 -1.8 101,150 - EX28DRAFT_3893 0.64 -0.6 101,303 - EX28DRAFT_3893 0.85 -0.6 101,349 + -2.9 101,446 + -2.5 101,448 + +0.2 101,465 + -0.9 101,466 - +2.0 101,494 + -2.7 101,495 - -0.5 101,497 - +0.0 101,532 + -0.4 101,544 + +1.0 101,577 + +1.6 101,678 + EX28DRAFT_3894 0.17 +2.6 101,679 - EX28DRAFT_3894 0.17 +0.7 101,694 + EX28DRAFT_3894 0.19 -0.1 101,694 + EX28DRAFT_3894 0.19 +0.7 101,695 - EX28DRAFT_3894 0.19 -2.0 101,724 + EX28DRAFT_3894 0.22 -0.2 101,724 + EX28DRAFT_3894 0.22 +0.4 101,725 - EX28DRAFT_3894 0.22 +0.3 101,856 + EX28DRAFT_3894 0.37 -0.4 101,856 + EX28DRAFT_3894 0.37 +0.0 101,861 + EX28DRAFT_3894 0.38 +0.2 101,862 - EX28DRAFT_3894 0.38 +0.5 102,052 - EX28DRAFT_3894 0.60 -0.1 102,062 + EX28DRAFT_3894 0.61 -0.4 102,062 + EX28DRAFT_3894 0.61 -0.3 102,165 + EX28DRAFT_3894 0.73 -0.8 102,166 - EX28DRAFT_3894 0.73 +2.2 102,166 - EX28DRAFT_3894 0.73 +1.2 102,217 + EX28DRAFT_3894 0.79 -0.5 102,218 - EX28DRAFT_3894 0.79 -0.7 102,264 + EX28DRAFT_3894 0.85 -0.2 102,264 + EX28DRAFT_3894 0.85 -0.6 102,264 + EX28DRAFT_3894 0.85 +1.7
Or see this region's nucleotide sequence