Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_3837

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_3836 and EX28DRAFT_3837 are separated by 240 nucleotidesEX28DRAFT_3837 and EX28DRAFT_3838 are separated by 4 nucleotidesEX28DRAFT_3838 and EX28DRAFT_3839 are separated by 93 nucleotides EX28DRAFT_3836: EX28DRAFT_3836 - Glycosidases, at 42,199 to 44,229 _3836 EX28DRAFT_3837: EX28DRAFT_3837 - valine-pyruvate aminotransferase apoenzyme (EC 2.6.1.66), at 44,470 to 45,726 _3837 EX28DRAFT_3838: EX28DRAFT_3838 - Fe-S-cluster-containing hydrogenase components 2, at 45,731 to 46,186 _3838 EX28DRAFT_3839: EX28DRAFT_3839 - Helix-turn-helix domain, at 46,280 to 46,691 _3839 Position (kb) 44 45 46Strain fitness (log2 ratio) -1 0 1 2at 43.503 kb on + strand, within EX28DRAFT_3836at 43.516 kb on + strand, within EX28DRAFT_3836at 43.876 kb on + strand, within EX28DRAFT_3836at 43.876 kb on + strand, within EX28DRAFT_3836at 43.958 kb on + strand, within EX28DRAFT_3836at 43.959 kb on - strand, within EX28DRAFT_3836at 43.966 kb on + strand, within EX28DRAFT_3836at 43.966 kb on + strand, within EX28DRAFT_3836at 43.969 kb on + strand, within EX28DRAFT_3836at 44.037 kb on + strandat 44.037 kb on + strandat 44.038 kb on - strandat 44.038 kb on - strandat 44.038 kb on - strandat 44.220 kb on + strandat 44.220 kb on + strandat 44.227 kb on + strandat 44.287 kb on - strandat 44.344 kb on - strandat 44.427 kb on + strandat 44.518 kb on - strandat 44.610 kb on + strand, within EX28DRAFT_3837at 44.610 kb on + strand, within EX28DRAFT_3837at 44.610 kb on + strand, within EX28DRAFT_3837at 44.611 kb on - strand, within EX28DRAFT_3837at 44.615 kb on - strand, within EX28DRAFT_3837at 44.677 kb on + strand, within EX28DRAFT_3837at 44.677 kb on + strand, within EX28DRAFT_3837at 44.695 kb on - strand, within EX28DRAFT_3837at 44.812 kb on - strand, within EX28DRAFT_3837at 44.878 kb on + strand, within EX28DRAFT_3837at 44.879 kb on - strand, within EX28DRAFT_3837at 44.879 kb on - strand, within EX28DRAFT_3837at 44.969 kb on - strand, within EX28DRAFT_3837at 44.995 kb on - strand, within EX28DRAFT_3837at 45.130 kb on + strand, within EX28DRAFT_3837at 45.153 kb on + strand, within EX28DRAFT_3837at 45.153 kb on + strand, within EX28DRAFT_3837at 45.154 kb on - strand, within EX28DRAFT_3837at 45.154 kb on - strand, within EX28DRAFT_3837at 45.397 kb on + strand, within EX28DRAFT_3837at 45.397 kb on + strand, within EX28DRAFT_3837at 45.398 kb on - strand, within EX28DRAFT_3837at 45.398 kb on - strand, within EX28DRAFT_3837at 45.398 kb on - strand, within EX28DRAFT_3837at 45.428 kb on + strand, within EX28DRAFT_3837at 45.600 kb on + strand, within EX28DRAFT_3837at 45.601 kb on - strandat 45.601 kb on - strandat 45.640 kb on + strandat 45.640 kb on + strandat 45.640 kb on + strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.641 kb on - strandat 45.842 kb on + strand, within EX28DRAFT_3838at 45.842 kb on + strand, within EX28DRAFT_3838at 45.843 kb on - strand, within EX28DRAFT_3838at 45.868 kb on + strand, within EX28DRAFT_3838at 45.868 kb on + strand, within EX28DRAFT_3838at 45.869 kb on - strand, within EX28DRAFT_3838at 45.916 kb on + strand, within EX28DRAFT_3838at 45.916 kb on + strand, within EX28DRAFT_3838at 46.047 kb on + strand, within EX28DRAFT_3838at 46.048 kb on - strand, within EX28DRAFT_3838at 46.207 kb on + strandat 46.207 kb on + strandat 46.207 kb on + strandat 46.231 kb on + strandat 46.231 kb on + strandat 46.232 kb on - strandat 46.270 kb on + strandat 46.270 kb on + strandat 46.270 kb on + strandat 46.270 kb on + strandat 46.291 kb on + strandat 46.292 kb on - strandat 46.294 kb on + strandat 46.294 kb on + strandat 46.295 kb on - strandat 46.295 kb on - strandat 46.295 kb on - strandat 46.308 kb on + strandat 46.334 kb on + strand, within EX28DRAFT_3839at 46.506 kb on + strand, within EX28DRAFT_3839at 46.507 kb on - strand, within EX28DRAFT_3839at 46.507 kb on - strand, within EX28DRAFT_3839at 46.519 kb on + strand, within EX28DRAFT_3839at 46.519 kb on + strand, within EX28DRAFT_3839at 46.520 kb on - strand, within EX28DRAFT_3839at 46.671 kb on + strandat 46.672 kb on - strandat 46.710 kb on - strandat 46.721 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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43,503 + EX28DRAFT_3836 0.64 -0.1
43,516 + EX28DRAFT_3836 0.65 +1.2
43,876 + EX28DRAFT_3836 0.83 +0.3
43,876 + EX28DRAFT_3836 0.83 -0.1
43,958 + EX28DRAFT_3836 0.87 -0.5
43,959 - EX28DRAFT_3836 0.87 +0.3
43,966 + EX28DRAFT_3836 0.87 +0.2
43,966 + EX28DRAFT_3836 0.87 -0.0
43,969 + EX28DRAFT_3836 0.87 +0.4
44,037 + -0.6
44,037 + -0.1
44,038 - +1.0
44,038 - -0.1
44,038 - +0.5
44,220 + +0.2
44,220 + +0.2
44,227 + +0.0
44,287 - -0.1
44,344 - +0.7
44,427 + +1.6
44,518 - +0.5
44,610 + EX28DRAFT_3837 0.11 -0.4
44,610 + EX28DRAFT_3837 0.11 -0.9
44,610 + EX28DRAFT_3837 0.11 +1.2
44,611 - EX28DRAFT_3837 0.11 -0.2
44,615 - EX28DRAFT_3837 0.12 +0.7
44,677 + EX28DRAFT_3837 0.16 -0.2
44,677 + EX28DRAFT_3837 0.16 -0.5
44,695 - EX28DRAFT_3837 0.18 +0.1
44,812 - EX28DRAFT_3837 0.27 -0.4
44,878 + EX28DRAFT_3837 0.32 -0.7
44,879 - EX28DRAFT_3837 0.33 -0.6
44,879 - EX28DRAFT_3837 0.33 -0.2
44,969 - EX28DRAFT_3837 0.40 +0.4
44,995 - EX28DRAFT_3837 0.42 -1.1
45,130 + EX28DRAFT_3837 0.53 -0.5
45,153 + EX28DRAFT_3837 0.54 -1.0
45,153 + EX28DRAFT_3837 0.54 -0.0
45,154 - EX28DRAFT_3837 0.54 +0.2
45,154 - EX28DRAFT_3837 0.54 +0.5
45,397 + EX28DRAFT_3837 0.74 +0.4
45,397 + EX28DRAFT_3837 0.74 -0.5
45,398 - EX28DRAFT_3837 0.74 -0.1
45,398 - EX28DRAFT_3837 0.74 -0.4
45,398 - EX28DRAFT_3837 0.74 +0.2
45,428 + EX28DRAFT_3837 0.76 -0.1
45,600 + EX28DRAFT_3837 0.90 -0.4
45,601 - +0.3
45,601 - -0.2
45,640 + +0.5
45,640 + +0.2
45,640 + +0.6
45,641 - +1.1
45,641 - +0.0
45,641 - -0.3
45,641 - +0.0
45,641 - +2.0
45,641 - -1.0
45,641 - +2.6
45,641 - +0.2
45,641 - +0.6
45,842 + EX28DRAFT_3838 0.24 +0.7
45,842 + EX28DRAFT_3838 0.24 -0.1
45,843 - EX28DRAFT_3838 0.25 +0.3
45,868 + EX28DRAFT_3838 0.30 -1.0
45,868 + EX28DRAFT_3838 0.30 +0.2
45,869 - EX28DRAFT_3838 0.30 +1.1
45,916 + EX28DRAFT_3838 0.41 -0.6
45,916 + EX28DRAFT_3838 0.41 +0.4
46,047 + EX28DRAFT_3838 0.69 +0.2
46,048 - EX28DRAFT_3838 0.70 -1.0
46,207 + +1.0
46,207 + -0.1
46,207 + +0.3
46,231 + +0.7
46,231 + +1.2
46,232 - +1.6
46,270 + -0.3
46,270 + +1.1
46,270 + +0.7
46,270 + +0.1
46,291 + -0.5
46,292 - -0.5
46,294 + +1.4
46,294 + -0.5
46,295 - -0.1
46,295 - -0.4
46,295 - +0.2
46,308 + +1.0
46,334 + EX28DRAFT_3839 0.13 -0.1
46,506 + EX28DRAFT_3839 0.55 +0.6
46,507 - EX28DRAFT_3839 0.55 -0.1
46,507 - EX28DRAFT_3839 0.55 -0.5
46,519 + EX28DRAFT_3839 0.58 +0.3
46,519 + EX28DRAFT_3839 0.58 -0.0
46,520 - EX28DRAFT_3839 0.58 -0.0
46,671 + +0.0
46,672 - -0.1
46,710 - +0.6
46,721 + -0.4

Or see this region's nucleotide sequence