Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3809 and EX28DRAFT_3810 overlap by 23 nucleotides EX28DRAFT_3810 and EX28DRAFT_3811 overlap by 38 nucleotides EX28DRAFT_3811 and EX28DRAFT_3812 are separated by 163 nucleotides EX28DRAFT_3812 and EX28DRAFT_3813 are separated by 129 nucleotides
EX28DRAFT_3809: EX28DRAFT_3809 - DNA-3-methyladenine glycosylase I (EC 3.2.2.20), at 13,397 to 13,978
_3809
EX28DRAFT_3810: EX28DRAFT_3810 - Acetyltransferase (GNAT) domain, at 13,956 to 14,402
_3810
EX28DRAFT_3811: EX28DRAFT_3811 - molybdopterin guanine dinucleotide-containing S/N-oxide reductases, at 14,365 to 16,692
_3811
EX28DRAFT_3812: EX28DRAFT_3812 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 16,856 to 17,518
_3812
EX28DRAFT_3813: EX28DRAFT_3813 - Transcriptional regulators, at 17,648 to 18,664
_3813
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -1
0
1 at 13.382 kb on + strand at 13.433 kb on + strand at 13.433 kb on + strand at 13.434 kb on - strand at 13.434 kb on - strand at 13.435 kb on + strand at 13.537 kb on + strand, within EX28DRAFT_3809 at 13.538 kb on - strand, within EX28DRAFT_3809 at 13.892 kb on - strand, within EX28DRAFT_3809 at 14.142 kb on - strand, within EX28DRAFT_3810 at 14.255 kb on - strand, within EX28DRAFT_3810 at 14.255 kb on - strand, within EX28DRAFT_3810 at 14.268 kb on + strand, within EX28DRAFT_3810 at 14.298 kb on + strand, within EX28DRAFT_3810 at 14.417 kb on + strand at 14.418 kb on - strand at 14.418 kb on - strand at 14.685 kb on + strand, within EX28DRAFT_3811 at 14.740 kb on - strand, within EX28DRAFT_3811 at 14.741 kb on + strand, within EX28DRAFT_3811 at 14.741 kb on + strand, within EX28DRAFT_3811 at 14.741 kb on + strand, within EX28DRAFT_3811 at 14.741 kb on + strand, within EX28DRAFT_3811 at 14.742 kb on - strand, within EX28DRAFT_3811 at 14.742 kb on - strand, within EX28DRAFT_3811 at 14.742 kb on - strand, within EX28DRAFT_3811 at 14.742 kb on - strand, within EX28DRAFT_3811 at 14.746 kb on - strand, within EX28DRAFT_3811 at 14.841 kb on + strand, within EX28DRAFT_3811 at 14.841 kb on + strand, within EX28DRAFT_3811 at 14.841 kb on + strand, within EX28DRAFT_3811 at 14.842 kb on - strand, within EX28DRAFT_3811 at 14.842 kb on - strand, within EX28DRAFT_3811 at 14.842 kb on - strand, within EX28DRAFT_3811 at 14.842 kb on - strand, within EX28DRAFT_3811 at 14.864 kb on + strand, within EX28DRAFT_3811 at 14.891 kb on + strand, within EX28DRAFT_3811 at 14.892 kb on - strand, within EX28DRAFT_3811 at 15.080 kb on + strand, within EX28DRAFT_3811 at 15.228 kb on + strand, within EX28DRAFT_3811 at 15.228 kb on + strand, within EX28DRAFT_3811 at 15.228 kb on + strand, within EX28DRAFT_3811 at 15.228 kb on + strand, within EX28DRAFT_3811 at 15.229 kb on - strand, within EX28DRAFT_3811 at 15.301 kb on - strand, within EX28DRAFT_3811 at 15.435 kb on + strand, within EX28DRAFT_3811 at 15.617 kb on + strand, within EX28DRAFT_3811 at 15.618 kb on - strand, within EX28DRAFT_3811 at 15.857 kb on + strand, within EX28DRAFT_3811 at 15.858 kb on - strand, within EX28DRAFT_3811 at 15.885 kb on + strand, within EX28DRAFT_3811 at 16.203 kb on - strand, within EX28DRAFT_3811 at 16.281 kb on - strand, within EX28DRAFT_3811 at 16.281 kb on - strand, within EX28DRAFT_3811 at 16.301 kb on + strand, within EX28DRAFT_3811 at 16.302 kb on - strand, within EX28DRAFT_3811 at 16.385 kb on + strand, within EX28DRAFT_3811 at 16.385 kb on + strand, within EX28DRAFT_3811 at 16.386 kb on - strand, within EX28DRAFT_3811 at 16.721 kb on - strand at 16.745 kb on - strand at 16.769 kb on + strand at 16.769 kb on + strand at 16.802 kb on + strand at 16.803 kb on - strand at 16.869 kb on + strand at 17.093 kb on + strand, within EX28DRAFT_3812 at 17.093 kb on + strand, within EX28DRAFT_3812 at 17.094 kb on - strand, within EX28DRAFT_3812 at 17.094 kb on - strand, within EX28DRAFT_3812 at 17.182 kb on + strand, within EX28DRAFT_3812 at 17.277 kb on - strand, within EX28DRAFT_3812 at 17.497 kb on + strand at 17.516 kb on + strand at 17.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 13,382 + -0.3 13,433 + +0.7 13,433 + -0.1 13,434 - -0.2 13,434 - +0.2 13,435 + +0.3 13,537 + EX28DRAFT_3809 0.24 +0.3 13,538 - EX28DRAFT_3809 0.24 -0.2 13,892 - EX28DRAFT_3809 0.85 -0.4 14,142 - EX28DRAFT_3810 0.42 +1.4 14,255 - EX28DRAFT_3810 0.67 +0.4 14,255 - EX28DRAFT_3810 0.67 +0.6 14,268 + EX28DRAFT_3810 0.70 -0.3 14,298 + EX28DRAFT_3810 0.77 +0.9 14,417 + -0.1 14,418 - +0.2 14,418 - +0.6 14,685 + EX28DRAFT_3811 0.14 +0.1 14,740 - EX28DRAFT_3811 0.16 -1.2 14,741 + EX28DRAFT_3811 0.16 -0.6 14,741 + EX28DRAFT_3811 0.16 -0.2 14,741 + EX28DRAFT_3811 0.16 +0.1 14,741 + EX28DRAFT_3811 0.16 +0.1 14,742 - EX28DRAFT_3811 0.16 +0.0 14,742 - EX28DRAFT_3811 0.16 -0.2 14,742 - EX28DRAFT_3811 0.16 -0.9 14,742 - EX28DRAFT_3811 0.16 +0.1 14,746 - EX28DRAFT_3811 0.16 +1.0 14,841 + EX28DRAFT_3811 0.20 -1.4 14,841 + EX28DRAFT_3811 0.20 -0.8 14,841 + EX28DRAFT_3811 0.20 +0.8 14,842 - EX28DRAFT_3811 0.20 +0.8 14,842 - EX28DRAFT_3811 0.20 -1.0 14,842 - EX28DRAFT_3811 0.20 +0.0 14,842 - EX28DRAFT_3811 0.20 -0.8 14,864 + EX28DRAFT_3811 0.21 +0.3 14,891 + EX28DRAFT_3811 0.23 +0.3 14,892 - EX28DRAFT_3811 0.23 +0.2 15,080 + EX28DRAFT_3811 0.31 +1.3 15,228 + EX28DRAFT_3811 0.37 -0.2 15,228 + EX28DRAFT_3811 0.37 -0.7 15,228 + EX28DRAFT_3811 0.37 -0.4 15,228 + EX28DRAFT_3811 0.37 -0.3 15,229 - EX28DRAFT_3811 0.37 +0.6 15,301 - EX28DRAFT_3811 0.40 -0.4 15,435 + EX28DRAFT_3811 0.46 +0.1 15,617 + EX28DRAFT_3811 0.54 -0.9 15,618 - EX28DRAFT_3811 0.54 +0.3 15,857 + EX28DRAFT_3811 0.64 +0.2 15,858 - EX28DRAFT_3811 0.64 -0.2 15,885 + EX28DRAFT_3811 0.65 -0.6 16,203 - EX28DRAFT_3811 0.79 -0.2 16,281 - EX28DRAFT_3811 0.82 +0.4 16,281 - EX28DRAFT_3811 0.82 -0.2 16,301 + EX28DRAFT_3811 0.83 -0.1 16,302 - EX28DRAFT_3811 0.83 +0.1 16,385 + EX28DRAFT_3811 0.87 +0.5 16,385 + EX28DRAFT_3811 0.87 +0.2 16,386 - EX28DRAFT_3811 0.87 +0.6 16,721 - +0.0 16,745 - +0.9 16,769 + -0.2 16,769 + +0.5 16,802 + -0.4 16,803 - +0.2 16,869 + -0.0 17,093 + EX28DRAFT_3812 0.36 +0.3 17,093 + EX28DRAFT_3812 0.36 +0.1 17,094 - EX28DRAFT_3812 0.36 +1.5 17,094 - EX28DRAFT_3812 0.36 +0.0 17,182 + EX28DRAFT_3812 0.49 +0.1 17,277 - EX28DRAFT_3812 0.63 -0.1 17,497 + +0.1 17,516 + +0.3 17,619 + -0.3
Or see this region's nucleotide sequence