Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3735 and EX28DRAFT_3736 overlap by 11 nucleotides EX28DRAFT_3736 and EX28DRAFT_3737 are separated by 73 nucleotides EX28DRAFT_3737 and EX28DRAFT_3738 are separated by 155 nucleotides
EX28DRAFT_3735: EX28DRAFT_3735 - sugar fermentation stimulation protein, at 409,603 to 410,307
_3735
EX28DRAFT_3736: EX28DRAFT_3736 - 2'-5' RNA ligase, at 410,297 to 410,851
_3736
EX28DRAFT_3737: EX28DRAFT_3737 - ATP-dependent helicase HrpB, at 410,925 to 413,354
_3737
EX28DRAFT_3738: EX28DRAFT_3738 - penicillin-binding protein 1B, at 413,510 to 416,035
_3738
Position (kb)
410
411
412
413
414 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.970 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 409.986 kb on + strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.077 kb on - strand, within EX28DRAFT_3735 at 410.112 kb on + strand, within EX28DRAFT_3735 at 410.113 kb on - strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.120 kb on + strand, within EX28DRAFT_3735 at 410.133 kb on + strand, within EX28DRAFT_3735 at 410.476 kb on + strand, within EX28DRAFT_3736 at 410.477 kb on - strand, within EX28DRAFT_3736 at 410.872 kb on - strand at 410.872 kb on - strand at 410.884 kb on + strand at 410.892 kb on - strand at 410.907 kb on - strand at 410.974 kb on + strand at 410.975 kb on - strand at 410.975 kb on - strand at 411.203 kb on + strand, within EX28DRAFT_3737 at 411.231 kb on - strand, within EX28DRAFT_3737 at 411.383 kb on + strand, within EX28DRAFT_3737 at 411.383 kb on + strand, within EX28DRAFT_3737 at 411.384 kb on - strand, within EX28DRAFT_3737 at 411.384 kb on - strand, within EX28DRAFT_3737 at 411.386 kb on + strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.387 kb on - strand, within EX28DRAFT_3737 at 411.840 kb on - strand, within EX28DRAFT_3737 at 411.917 kb on - strand, within EX28DRAFT_3737 at 411.917 kb on - strand, within EX28DRAFT_3737 at 411.917 kb on - strand, within EX28DRAFT_3737 at 412.076 kb on - strand, within EX28DRAFT_3737 at 412.355 kb on + strand, within EX28DRAFT_3737 at 412.863 kb on + strand, within EX28DRAFT_3737 at 412.865 kb on + strand, within EX28DRAFT_3737 at 412.865 kb on + strand, within EX28DRAFT_3737 at 412.865 kb on + strand, within EX28DRAFT_3737 at 412.866 kb on - strand, within EX28DRAFT_3737 at 412.866 kb on - strand, within EX28DRAFT_3737 at 412.866 kb on - strand, within EX28DRAFT_3737 at 412.866 kb on - strand, within EX28DRAFT_3737 at 412.866 kb on - strand, within EX28DRAFT_3737 at 412.940 kb on - strand, within EX28DRAFT_3737 at 412.993 kb on + strand, within EX28DRAFT_3737 at 412.993 kb on + strand, within EX28DRAFT_3737 at 413.043 kb on + strand, within EX28DRAFT_3737 at 413.043 kb on + strand, within EX28DRAFT_3737 at 413.043 kb on + strand, within EX28DRAFT_3737 at 413.044 kb on - strand, within EX28DRAFT_3737 at 413.044 kb on - strand, within EX28DRAFT_3737 at 413.044 kb on - strand, within EX28DRAFT_3737 at 413.209 kb on + strand at 413.210 kb on - strand at 413.346 kb on + strand at 413.347 kb on - strand at 413.352 kb on + strand at 413.352 kb on + strand at 413.353 kb on - strand at 413.357 kb on + strand at 413.357 kb on + strand at 413.357 kb on + strand at 413.358 kb on - strand at 413.536 kb on - strand at 413.542 kb on + strand at 413.577 kb on + strand at 413.577 kb on + strand at 413.606 kb on + strand at 413.606 kb on + strand at 413.606 kb on + strand at 413.618 kb on + strand at 413.725 kb on + strand at 413.812 kb on - strand, within EX28DRAFT_3738 at 413.854 kb on + strand, within EX28DRAFT_3738 at 413.854 kb on + strand, within EX28DRAFT_3738 at 413.854 kb on + strand, within EX28DRAFT_3738 at 413.854 kb on + strand, within EX28DRAFT_3738 at 413.855 kb on - strand, within EX28DRAFT_3738 at 413.855 kb on - strand, within EX28DRAFT_3738 at 414.218 kb on + strand, within EX28DRAFT_3738 at 414.243 kb on - strand, within EX28DRAFT_3738
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 409,970 + EX28DRAFT_3735 0.52 +1.1 409,970 + EX28DRAFT_3735 0.52 +0.7 409,986 + EX28DRAFT_3735 0.54 -0.7 409,986 + EX28DRAFT_3735 0.54 +0.3 410,077 - EX28DRAFT_3735 0.67 -0.2 410,077 - EX28DRAFT_3735 0.67 +1.1 410,112 + EX28DRAFT_3735 0.72 -0.2 410,113 - EX28DRAFT_3735 0.72 -0.3 410,120 + EX28DRAFT_3735 0.73 -0.4 410,120 + EX28DRAFT_3735 0.73 +1.3 410,120 + EX28DRAFT_3735 0.73 +0.3 410,133 + EX28DRAFT_3735 0.75 +0.1 410,476 + EX28DRAFT_3736 0.32 -0.0 410,477 - EX28DRAFT_3736 0.32 +0.1 410,872 - -0.0 410,872 - -0.1 410,884 + -0.4 410,892 - -1.3 410,907 - -0.1 410,974 + -0.5 410,975 - -1.9 410,975 - -0.6 411,203 + EX28DRAFT_3737 0.11 +0.7 411,231 - EX28DRAFT_3737 0.13 -0.1 411,383 + EX28DRAFT_3737 0.19 -0.0 411,383 + EX28DRAFT_3737 0.19 -0.9 411,384 - EX28DRAFT_3737 0.19 +0.2 411,384 - EX28DRAFT_3737 0.19 +0.7 411,386 + EX28DRAFT_3737 0.19 +0.8 411,387 - EX28DRAFT_3737 0.19 -0.4 411,387 - EX28DRAFT_3737 0.19 +0.6 411,387 - EX28DRAFT_3737 0.19 +1.0 411,387 - EX28DRAFT_3737 0.19 +0.2 411,387 - EX28DRAFT_3737 0.19 -0.2 411,387 - EX28DRAFT_3737 0.19 -0.2 411,840 - EX28DRAFT_3737 0.38 -0.1 411,917 - EX28DRAFT_3737 0.41 +0.8 411,917 - EX28DRAFT_3737 0.41 -0.5 411,917 - EX28DRAFT_3737 0.41 -0.2 412,076 - EX28DRAFT_3737 0.47 -0.3 412,355 + EX28DRAFT_3737 0.59 +0.0 412,863 + EX28DRAFT_3737 0.80 +1.3 412,865 + EX28DRAFT_3737 0.80 +0.2 412,865 + EX28DRAFT_3737 0.80 -0.4 412,865 + EX28DRAFT_3737 0.80 +0.6 412,866 - EX28DRAFT_3737 0.80 +0.5 412,866 - EX28DRAFT_3737 0.80 -3.9 412,866 - EX28DRAFT_3737 0.80 -0.0 412,866 - EX28DRAFT_3737 0.80 +0.3 412,866 - EX28DRAFT_3737 0.80 -0.4 412,940 - EX28DRAFT_3737 0.83 +0.5 412,993 + EX28DRAFT_3737 0.85 +0.0 412,993 + EX28DRAFT_3737 0.85 +0.5 413,043 + EX28DRAFT_3737 0.87 -0.4 413,043 + EX28DRAFT_3737 0.87 -0.9 413,043 + EX28DRAFT_3737 0.87 +0.7 413,044 - EX28DRAFT_3737 0.87 -0.1 413,044 - EX28DRAFT_3737 0.87 -0.1 413,044 - EX28DRAFT_3737 0.87 -0.3 413,209 + -0.1 413,210 - -0.7 413,346 + +0.2 413,347 - +0.7 413,352 + +0.1 413,352 + +1.1 413,353 - +1.0 413,357 + +1.3 413,357 + -1.4 413,357 + -1.3 413,358 - +0.3 413,536 - +1.0 413,542 + -0.2 413,577 + -1.5 413,577 + -1.4 413,606 + +0.2 413,606 + -0.2 413,606 + +0.3 413,618 + -0.1 413,725 + -1.0 413,812 - EX28DRAFT_3738 0.12 -0.3 413,854 + EX28DRAFT_3738 0.14 +0.9 413,854 + EX28DRAFT_3738 0.14 +1.7 413,854 + EX28DRAFT_3738 0.14 -0.4 413,854 + EX28DRAFT_3738 0.14 +0.7 413,855 - EX28DRAFT_3738 0.14 -0.3 413,855 - EX28DRAFT_3738 0.14 +0.0 414,218 + EX28DRAFT_3738 0.28 +0.4 414,243 - EX28DRAFT_3738 0.29 +1.0
Or see this region's nucleotide sequence