Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3730 and EX28DRAFT_3731 are separated by 123 nucleotides EX28DRAFT_3731 and EX28DRAFT_3732 overlap by 4 nucleotides EX28DRAFT_3732 and EX28DRAFT_3733 are separated by 75 nucleotides
EX28DRAFT_3730: EX28DRAFT_3730 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 405,190 to 405,981
_3730
EX28DRAFT_3731: EX28DRAFT_3731 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 406,105 to 406,584
_3731
EX28DRAFT_3732: EX28DRAFT_3732 - poly(A) polymerase, at 406,581 to 407,945
_3732
EX28DRAFT_3733: EX28DRAFT_3733 - glutamyl-queuosine tRNA(Asp) synthetase, at 408,021 to 408,911
_3733
Position (kb) 406
407
408 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 405.778 kb on - strand, within EX28DRAFT_3730 at 405.902 kb on + strand at 405.903 kb on - strand at 405.909 kb on - strand at 405.959 kb on - strand at 405.981 kb on + strand at 406.029 kb on - strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.053 kb on - strand at 406.053 kb on - strand at 406.056 kb on + strand at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 407.910 kb on - strand at 407.991 kb on - strand at 407.991 kb on - strand at 408.025 kb on - strand at 408.138 kb on + strand, within EX28DRAFT_3733 at 408.268 kb on + strand, within EX28DRAFT_3733 at 408.299 kb on - strand, within EX28DRAFT_3733 at 408.306 kb on + strand, within EX28DRAFT_3733 at 408.307 kb on - strand, within EX28DRAFT_3733 at 408.396 kb on - strand, within EX28DRAFT_3733 at 408.397 kb on + strand, within EX28DRAFT_3733 at 408.397 kb on + strand, within EX28DRAFT_3733 at 408.398 kb on - strand, within EX28DRAFT_3733 at 408.398 kb on - strand, within EX28DRAFT_3733 at 408.428 kb on + strand, within EX28DRAFT_3733 at 408.429 kb on - strand, within EX28DRAFT_3733 at 408.429 kb on - strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.515 kb on + strand, within EX28DRAFT_3733 at 408.516 kb on - strand, within EX28DRAFT_3733 at 408.520 kb on - strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.616 kb on + strand, within EX28DRAFT_3733 at 408.617 kb on - strand, within EX28DRAFT_3733 at 408.823 kb on + strand at 408.843 kb on + strand at 408.859 kb on - strand at 408.859 kb on - strand at 408.894 kb on - strand at 408.895 kb on + strand at 408.896 kb on - strand at 408.896 kb on - strand at 408.911 kb on + strand at 408.911 kb on + strand at 408.911 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 405,778 - EX28DRAFT_3730 0.74 -1.7 405,902 + -0.3 405,903 - +0.4 405,909 - -0.0 405,959 - -2.4 405,981 + -1.3 406,029 - -0.0 406,052 + +0.3 406,052 + +1.3 406,052 + -0.2 406,053 - +0.7 406,053 - -1.9 406,056 + -0.7 406,955 - EX28DRAFT_3732 0.27 +1.3 406,955 - EX28DRAFT_3732 0.27 +0.0 406,955 - EX28DRAFT_3732 0.27 -0.9 407,910 - -1.7 407,991 - +0.5 407,991 - -1.6 408,025 - +0.6 408,138 + EX28DRAFT_3733 0.13 +0.1 408,268 + EX28DRAFT_3733 0.28 +1.2 408,299 - EX28DRAFT_3733 0.31 -1.6 408,306 + EX28DRAFT_3733 0.32 +0.7 408,307 - EX28DRAFT_3733 0.32 -1.0 408,396 - EX28DRAFT_3733 0.42 -0.1 408,397 + EX28DRAFT_3733 0.42 +0.3 408,397 + EX28DRAFT_3733 0.42 -0.2 408,398 - EX28DRAFT_3733 0.42 +3.3 408,398 - EX28DRAFT_3733 0.42 -0.1 408,428 + EX28DRAFT_3733 0.46 -1.2 408,429 - EX28DRAFT_3733 0.46 -0.4 408,429 - EX28DRAFT_3733 0.46 +0.7 408,515 + EX28DRAFT_3733 0.55 +0.3 408,515 + EX28DRAFT_3733 0.55 +0.3 408,515 + EX28DRAFT_3733 0.55 +0.1 408,516 - EX28DRAFT_3733 0.56 +0.2 408,520 - EX28DRAFT_3733 0.56 +1.5 408,616 + EX28DRAFT_3733 0.67 +0.2 408,616 + EX28DRAFT_3733 0.67 +1.1 408,616 + EX28DRAFT_3733 0.67 +0.1 408,617 - EX28DRAFT_3733 0.67 +1.1 408,823 + +0.9 408,843 + -2.0 408,859 - +0.4 408,859 - +0.2 408,894 - +0.3 408,895 + +0.7 408,896 - -0.3 408,896 - -2.3 408,911 + +0.0 408,911 + +0.4 408,911 + +0.4
Or see this region's nucleotide sequence